Caplugs® Evergreen
0.1–0.45 mL Deep Well Microplates, PPC, Natural — 290-8538-080 | 5/Bag | Caplugs® Evergreen
0.1–0.45 mL Deep Well Microplates, PPC, Natural — 290-8538-080 | 5/Bag | Caplugs® Evergreen
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96-Well Deep Well Microplates, Tapered Bottom, 0.5 mL Well Volume, Polypropylene Copolymer (PPC), Natural — 290-8538-080 | 5/Bag (20/Case)
| Quick Reference — 290-8538-080 | |
|---|---|
| Well Format | 96-well SBS/ANSI |
| Bottom | Tapered |
| Well Volume | 0.5 mL — 5× standard 96-well |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| DMSO Compat. | ✓ All concentrations (PPC) |
| Autoclave | ✓ Compatible (PPC) |
| Primary Use | High-volume 96-well sample preparation |
| Pack Size | 5/Bag (20/Case) |
| Price | $83.72 / Bag ($4.1856 / plate) |
Technical Narrative
The Caplugs® Evergreen is a 96-well deep well Polypropylene Copolymer (PPC) microplate with tapered bottom wells providing 0.5 mL per well — five times the capacity of standard 96-well plates — in the same SBS/ANSI standard footprint compatible with all standard 96-well plate readers, liquid handlers, and centrifuge adapters. Deep well plates address the most common volume constraint in 96-well sample preparation: the standard 96-well well volume (~0.37 mL) is insufficient for high-volume lysis buffer incubation, large-volume DNA extraction protocols, pre-PCR sample normalization, and biobank aliquoting workflows requiring >0.3 mL per well. The tapered bottom enables complete aspiration of well contents without residual volume loss at the well base.
PPC construction extends standard deep well plate applications to chemically demanding workflows: full DMSO compatibility for compound-containing sample matrices, -80°C ultra-low temperature storage for deep well biobank archive plates, autoclave compatibility for pre-sterilized deep well supply, and broader organic solvent resistance for extraction and precipitation workflows. PPC's copolymer toughness maintains the tapered well geometry through repeated freeze-thaw cycling at -80°C — critical for deep well archive plates that cycle between cryogenic storage and ambient temperature aliquoting.
At $83.72 per 5/Bag — $4.1856 per plate — the delivers deep well PPC capacity at per-plate economics for high-volume sample prep programs. Also available: 290-8525-010 (PS Natural).
Technical Specifications — Caplugs® Evergreen
| Specification | Value |
|---|---|
| Part Number | |
| Caplugs Item # | 2908538080E1 |
| Well Format | 96-well SBS/ANSI |
| Bottom | Tapered |
| Well Volume | 0.5 mL per well (5× standard 96-well volume) |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| DMSO Compat. | ✓ All concentrations (PPC) |
| Autoclave | ✓ Compatible (121°C) |
| vs companion | 290-8525-010 (PS Natural) — same geometry, different material |
| Pack Size | 5/Bag (20/Case) |
| BPA/Phthalate | BPA-free; no phthalate plasticizers |
| Country of Origin | Made in U.S.A. (California) |
96-Well Deep Well Plate Workflow Compatibility — PPC 0.5 mL
96-well deep well plates provide 5× the volume of standard 96-well wells in the same SBS footprint. Key applications for the PPC format:
| Application | Assay / Use | Notes |
|---|---|---|
| Sample preparation (0.5 mL) | DNA extraction, protein digestion, cell lysis | 0.5 mL deep-well provides 5× more volume vs standard 96-well |
| High-volume ELISA | Large-volume antibody dilution and sample prep | 0.5 mL volume for pre-dilution steps before transfer to assay plate |
| Cell lysis and extract prep | High-volume lysis buffer incubation, centrifugation | Deep well volume for complete lysis without overflow |
| PCR pre-preparation | Master mix setup, DNA normalization, dilution | 0.5 mL enables higher-volume pre-PCR sample prep in 96-well format |
| Compound dilution series | Stock dilution before transfer to assay plates | Deep well for high-volume dilution series before acoustic dispensing |
| Protein expression harvest | Cell culture supernatant harvest from 96-well | Deep well accommodates larger culture volume per well |
| Biobank aliquoting | Sample aliquoting into deep-well archive plates | 0.5 mL per well for larger-volume biobank aliquot archiving |
| Filtration / centrifugation prep | Sample cleanup before 96-well transfer | Deep well paired with filter plates for cleanup workflows |
Working volume is typically 0.1–0.45 mL per well — do not overfill above 0.5 mL max. Tapered bottom enables complete aspiration. PPC: choose when DMSO, organic solvents, or -80°C storage are required.
Chemical Resistance — Polypropylene Copolymer (PPC)
PPC provides full DMSO compatibility, autoclave capability, and -80°C cryo stability for deep-well applications.
| Reagent / Condition | Compatibility |
|---|---|
| DMSO (all concentrations) | ✓ Excellent |
| Aqueous buffers / media | ✓ Excellent |
| Short-chain alcohols | ✓ Excellent |
| Concentrated organic solvents | ✓ Good |
| Dilute acids / alkalies | ✓ Excellent |
| Storage at -80°C | ✓ Suitable |
| Autoclave (121°C / 15 psi) | ✓ Compatible |
Laboratory Applications
The serves genomics and molecular biology programs performing high-volume DNA/RNA extraction in 96-well format; proteomics programs running protein digestion and cleanup; clinical diagnostics for large-volume sample preparation; biorepository programs requiring high-volume per-well aliquoting; pharmaceutical programs storing DMSO compound-containing samples at -80°C; PK/PD programs processing plasma samples with organic solvent protein precipitation; and any application requiring 5× standard 96-well volume in a standard SBS footprint.
U.S. Manufacturing, Quality & Institutional Procurement
Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.
Frequently Asked Questions — Caplugs® Evergreen
What is the advantage of deep well plates over standard 96-well plates?
Deep well plates provide 0.5 mL per well versus ~0.37 mL in standard 96-well — approximately 5× more volume in the same SBS footprint. This eliminates the need to use multiple standard wells per sample, enables high-volume lysis (400+ µL lysis buffer per well), accommodates large-volume DNA extraction protocols, and provides sufficient working volume for pre-PCR normalization and dilution series in a single deep well rather than splitting across multiple standard wells.
When should I use PPC deep well instead of PS (290-8525-010)?
Choose PPC when: (1) DMSO is present in the sample matrix or extraction buffer; (2) Plates will be stored at -80°C for long-term deep well archiving; (3) Organic solvents (chloroform, hexane, methanol) are used in the extraction protocol; (4) Autoclave sterilization before use is required. PS (290-8525-010) is the cost-effective choice for standard aqueous DNA/RNA extraction and protein digestion without DMSO or cryogenic storage requirements.
Are the deep well plates BPA-free and compatible with centrifugation?
Yes — BPA-free, no phthalates. Polypropylene Copolymer (PPC) deep well plates are compatible with standard centrifuge adapters for 96-well SBS-footprint plates. Confirm the g-force rating with your centrifuge rotor manufacturer before spinning — deep well plate walls are thicker than standard plates but have specific g-force limits depending on sample volume and fill level. Typical use: 500–3,000 × g for pelleting and clarification.
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