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Caplugs® Evergreen

0.1–0.45 mL Deep Well Microplates, PPC, Natural — 290-8538-080 | 5/Bag | Caplugs® Evergreen

0.1–0.45 mL Deep Well Microplates, PPC, Natural — 290-8538-080 | 5/Bag | Caplugs® Evergreen

Regular price $83.72
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96-Well Deep Well Microplates, Tapered Bottom, 0.5 mL Well Volume, Polypropylene Copolymer (PPC), Natural — 290-8538-080 | 5/Bag (20/Case)

Quick Reference — 290-8538-080
Well Format 96-well SBS/ANSI
Bottom Tapered
Well Volume 0.5 mL — 5× standard 96-well
Material Polypropylene Copolymer (PPC)
Color Natural
DMSO Compat. ✓ All concentrations (PPC)
Autoclave ✓ Compatible (PPC)
Primary Use High-volume 96-well sample preparation
Pack Size 5/Bag (20/Case)
Price $83.72 / Bag ($4.1856 / plate)

Technical Narrative

The Caplugs® Evergreen is a 96-well deep well Polypropylene Copolymer (PPC) microplate with tapered bottom wells providing 0.5 mL per well — five times the capacity of standard 96-well plates — in the same SBS/ANSI standard footprint compatible with all standard 96-well plate readers, liquid handlers, and centrifuge adapters. Deep well plates address the most common volume constraint in 96-well sample preparation: the standard 96-well well volume (~0.37 mL) is insufficient for high-volume lysis buffer incubation, large-volume DNA extraction protocols, pre-PCR sample normalization, and biobank aliquoting workflows requiring >0.3 mL per well. The tapered bottom enables complete aspiration of well contents without residual volume loss at the well base.

PPC construction extends standard deep well plate applications to chemically demanding workflows: full DMSO compatibility for compound-containing sample matrices, -80°C ultra-low temperature storage for deep well biobank archive plates, autoclave compatibility for pre-sterilized deep well supply, and broader organic solvent resistance for extraction and precipitation workflows. PPC's copolymer toughness maintains the tapered well geometry through repeated freeze-thaw cycling at -80°C — critical for deep well archive plates that cycle between cryogenic storage and ambient temperature aliquoting.

At $83.72 per 5/Bag — $4.1856 per plate — the delivers deep well PPC capacity at per-plate economics for high-volume sample prep programs. Also available: 290-8525-010 (PS Natural).

Technical Specifications — Caplugs® Evergreen

Specification Value
Part Number
Caplugs Item # 2908538080E1
Well Format 96-well SBS/ANSI
Bottom Tapered
Well Volume 0.5 mL per well (5× standard 96-well volume)
Material Polypropylene Copolymer (PPC)
Color Natural
DMSO Compat. ✓ All concentrations (PPC)
Autoclave ✓ Compatible (121°C)
vs companion 290-8525-010 (PS Natural) — same geometry, different material
Pack Size 5/Bag (20/Case)
BPA/Phthalate BPA-free; no phthalate plasticizers
Country of Origin Made in U.S.A. (California)

96-Well Deep Well Plate Workflow Compatibility — PPC 0.5 mL

96-well deep well plates provide 5× the volume of standard 96-well wells in the same SBS footprint. Key applications for the PPC format:

Application Assay / Use Notes
Sample preparation (0.5 mL) DNA extraction, protein digestion, cell lysis 0.5 mL deep-well provides 5× more volume vs standard 96-well
High-volume ELISA Large-volume antibody dilution and sample prep 0.5 mL volume for pre-dilution steps before transfer to assay plate
Cell lysis and extract prep High-volume lysis buffer incubation, centrifugation Deep well volume for complete lysis without overflow
PCR pre-preparation Master mix setup, DNA normalization, dilution 0.5 mL enables higher-volume pre-PCR sample prep in 96-well format
Compound dilution series Stock dilution before transfer to assay plates Deep well for high-volume dilution series before acoustic dispensing
Protein expression harvest Cell culture supernatant harvest from 96-well Deep well accommodates larger culture volume per well
Biobank aliquoting Sample aliquoting into deep-well archive plates 0.5 mL per well for larger-volume biobank aliquot archiving
Filtration / centrifugation prep Sample cleanup before 96-well transfer Deep well paired with filter plates for cleanup workflows

Working volume is typically 0.1–0.45 mL per well — do not overfill above 0.5 mL max. Tapered bottom enables complete aspiration. PPC: choose when DMSO, organic solvents, or -80°C storage are required.

Chemical Resistance — Polypropylene Copolymer (PPC)

PPC provides full DMSO compatibility, autoclave capability, and -80°C cryo stability for deep-well applications.

Reagent / Condition Compatibility
DMSO (all concentrations) ✓ Excellent
Aqueous buffers / media ✓ Excellent
Short-chain alcohols ✓ Excellent
Concentrated organic solvents ✓ Good
Dilute acids / alkalies ✓ Excellent
Storage at -80°C ✓ Suitable
Autoclave (121°C / 15 psi) ✓ Compatible

Laboratory Applications

The serves genomics and molecular biology programs performing high-volume DNA/RNA extraction in 96-well format; proteomics programs running protein digestion and cleanup; clinical diagnostics for large-volume sample preparation; biorepository programs requiring high-volume per-well aliquoting; pharmaceutical programs storing DMSO compound-containing samples at -80°C; PK/PD programs processing plasma samples with organic solvent protein precipitation; and any application requiring 5× standard 96-well volume in a standard SBS footprint.

U.S. Manufacturing, Quality & Institutional Procurement

Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.

Frequently Asked Questions — Caplugs® Evergreen

What is the advantage of deep well plates over standard 96-well plates?

Deep well plates provide 0.5 mL per well versus ~0.37 mL in standard 96-well — approximately 5× more volume in the same SBS footprint. This eliminates the need to use multiple standard wells per sample, enables high-volume lysis (400+ µL lysis buffer per well), accommodates large-volume DNA extraction protocols, and provides sufficient working volume for pre-PCR normalization and dilution series in a single deep well rather than splitting across multiple standard wells.

When should I use PPC deep well instead of PS (290-8525-010)?

Choose PPC when: (1) DMSO is present in the sample matrix or extraction buffer; (2) Plates will be stored at -80°C for long-term deep well archiving; (3) Organic solvents (chloroform, hexane, methanol) are used in the extraction protocol; (4) Autoclave sterilization before use is required. PS (290-8525-010) is the cost-effective choice for standard aqueous DNA/RNA extraction and protein digestion without DMSO or cryogenic storage requirements.

Are the deep well plates BPA-free and compatible with centrifugation?

Yes — BPA-free, no phthalates. Polypropylene Copolymer (PPC) deep well plates are compatible with standard centrifuge adapters for 96-well SBS-footprint plates. Confirm the g-force rating with your centrifuge rotor manufacturer before spinning — deep well plate walls are thicker than standard plates but have specific g-force limits depending on sample volume and fill level. Typical use: 500–3,000 × g for pelleting and clarification.

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