96-Well V-Bottom Microplates, 0.30 mL, Polypropylene — Conical (V-Bottom), Natural, 10/Box (290-8355-03V)
These 96-well polypropylene V-bottom microplates deliver a 0.30 mL conical well geometry that concentrates every cell, bead, or particle at the geometric apex for complete pellet recovery and clean supernatant removal. Supplied natural (translucent) in SBS/ANSI footprint format, part number 290-8355-03V is the preferred choice when workflows demand DMSO compatibility at all concentrations, broad organic solvent resistance, or the ability to autoclave plates before use — capabilities that polystyrene formats simply cannot provide. Each box contains 10 plates, and cases of 100 plates are available for high-throughput procurement.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 290-8355-03V |
| Well Format | 96-Well SBS/ANSI |
| Well Bottom Style | Conical (V-Bottom) |
| Well Volume | 0.30 mL |
| Material | Polypropylene (PP) |
| Color | Natural (translucent) |
| DMSO Compatibility | All concentrations |
| Autoclave Compatibility | Yes — 121 °C / 15 psi |
| BPA / Phthalates | BPA-free; no phthalate plasticizers |
| Pack Size | 10/Box (100/Case) |
| Country of Origin | USA |
Applications
- Acoustic dispensing source plates: DMSO compound stocks cycle repeatedly through the same plate in Echo® and Mosquito® acoustic dispensing programs; polypropylene withstands undiluted DMSO without well degradation.
- Compound library management: Store and reformat DMSO-dissolved compound libraries where the V-bottom apex ensures complete aspiration with minimal dead volume.
- Protein precipitation and pellet recovery: Precipitate proteins from DMSO-containing or solvent-phase samples and recover complete pellets at the well apex.
- Liquid-liquid extraction: Perform organic-phase separations using chloroform, hexane, or ethyl acetate — solvents that are incompatible with polystyrene plates.
- Magnetic bead pull-downs in non-aqueous matrices: Recover magnetic beads from DMSO or alcohol matrices with full visual confirmation of pellet position at the apex.
- Drug compound solubility screening: Run solubility assays in organic matrices using a format that resists solvent attack across the full concentration range.
- Autoclave-sterilized V-bottom workflows: Pre-sterilize plates for regulated pelleting assays, blood typing with detergent-containing reagents, or RBC sedimentation protocols requiring sterile conditions.
- Acid precipitation and alkaline lysis: Polypropylene's resistance to concentrated acids and alkalis extends the plate to pH-extreme sample preparation steps that would degrade polystyrene.
Compatibility
The 96-well SBS/ANSI footprint is compatible with standard robotic liquid handlers, plate stackers, centrifuges, and acoustic dispensers designed around the universal microplate format. For aqueous-only workflows — ELISA, standard cell-based assays, and sample storage without organic solvents — the polystyrene V-bottom plate (290-8116-01V) is an alternative at a comparable price point. Choose these PP plates (290-8355-03V) whenever DMSO, organic solvents, elevated temperature, or autoclave sterilization is part of the protocol; choose the PS variant when working exclusively with aqueous buffers and cost-per-plate is the primary driver.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| DMSO — all concentrations | Excellent |
| Aqueous buffers / saline / PBS | Excellent |
| Dilute acids and concentrated acids | Excellent |
| Dilute and concentrated alkalis | Excellent |
| Ethanol / isopropanol / methanol | Good — short-term contact |
| Chloroform / ethyl acetate / hexane | Good — suitable for liquid-liquid extraction contact times |
| Acetone (prolonged exposure) | Limited — avoid extended contact |
| Autoclave (121 °C / 15 psi) | Compatible |
Quality & Procurement
Made in USA, these Caplugs® Evergreen microplates are manufactured from BPA-free, phthalate-free polypropylene under ISO 9001 quality management, ensuring lot-to-lot dimensional consistency critical for robotic liquid-handling integration. LabSupplies.com is an authorized Caplugs® Evergreen dealer shipping from USA inventory; case quantities of 100 plates are available for high-volume screening programs, and institutional purchase orders are welcome.
Frequently Asked Questions
Can these plates be autoclaved for sterile workflows?
Yes. The polypropylene construction is autoclave-compatible at 121 °C / 15 psi, making pre-sterilized V-bottom plates practical for regulated assays, sterile cell pelleting, and blood typing protocols that require a clean plate supply.
What is the difference between this PP plate and the PS V-bottom plate (290-8116-01V)?
Both share the 96-well V-bottom geometry and 0.30 mL well volume, but the materials are not interchangeable for all workflows. This polypropylene plate tolerates DMSO at all concentrations, broad organic solvents, and autoclave sterilization. The polystyrene variant is well suited to standard aqueous applications but should not be used with DMSO, chlorinated or aromatic solvents, or in an autoclave.
Are these plates suitable for acoustic dispensing programs such as Echo® or Mosquito®?
Yes. Polypropylene's full DMSO compatibility makes these plates the standard source-plate material for acoustic dispensing programs where compound stocks are stored and repeatedly dispensed in DMSO. The V-bottom apex also ensures complete aspiration of small residual volumes, minimizing dead volume and compound waste.
Are these microplates sterile as shipped?
No — they are supplied non-sterile. For sterile applications, the plates can be autoclaved by the end user at 121 °C / 15 psi prior to use, or they can be purchased in case quantities and autoclaved in-house as part of your standard preparation workflow.