24-Well Flat-Bottom Microplates, 3.5 mL Well Volume — Polystyrene, Natural, 10/Bag (290-8124-01F)
These 24-well flat-bottom microplates from Caplugs® Evergreen conform to the SBS/ANSI standard footprint and are manufactured from natural (transparent) polystyrene, providing 3.5 mL well volume per well with a working volume of approximately 1–2.5 mL. Supplied non-sterile in bags of 10 (100/Case), they are designed for adherent cell culture, virus plaque assays, transfection, and other applications that demand greater well volume and surface area than the 96-well format can accommodate. The flat-bottom geometry supports consistent optical imaging of cell monolayers and is compatible with 15 mm round coverslip placement for confocal and widefield fluorescence microscopy.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 290-8124-01F |
| Well Format | 24-Well |
| Footprint | SBS/ANSI Standard |
| Bottom Geometry | Flat Bottom |
| Well Volume | 3.5 mL per well |
| Working Volume | ~1–2.5 mL per well |
| Well Inner Diameter | ~15.5 mm |
| Material | Polystyrene (PS) |
| Color | Natural (transparent) |
| Sterility | Non-Sterile |
| Pack Size | 10/Bag (100/Case) |
| BPA / Phthalates | BPA-free; no phthalate plasticizers |
| Country of Origin | USA |
Applications
- Adherent cell culture: Primary cells, fibroblasts, and epithelial cell lines benefit from the 3.5 mL well volume, which comfortably supports 1–2 mL of culture medium with adequate headspace.
- Virus plaque assay and TCID50 titration: The 24-well format is the WHO-recommended and ATCC-standard format for plaque assay and cytopathic effect (CPE) scoring; the large well surface area supports clear plaque visualization.
- Transfection and transduction: Plasmid, siRNA, and lentiviral delivery at 24-well scale — ideal for scaling up from 96-well optimization before progressing to larger formats.
- Primary neuron culture: Cortical, hippocampal, and DRG neuron culture; the ~15.5 mm inner well diameter accommodates 15 mm round coverslips for high-magnification imaging of neurons grown directly in the well.
- Organoid embedding: Compatible with Matrigel and basement membrane extract embedding protocols at working volumes supported by the 3.5 mL well capacity.
- Cell-based dose-response assays: Sufficient well volume and surface area for multi-concentration drug treatment studies on adherent cell monolayers.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline / PBS | Excellent |
| Standard cell culture media (DMEM, RPMI, MEM) | Excellent |
| DMSO (≤10%) | Compatible at working concentrations |
| Common fixatives (formaldehyde, paraformaldehyde) at working concentrations | Compatible — short-term contact |
| Ethanol >70% / methanol | Not recommended |
| Acetone / chloroform / aromatic solvents | Not recommended |
| Autoclaving | Not suitable — PS is not autoclavable |
Quality & Procurement
Made in USA, these Caplugs® Evergreen microplates are manufactured from BPA-free, phthalate-free polystyrene resin under ISO 9001 quality management standards. The natural (transparent) PS construction provides excellent optical clarity for visual inspection and plate-reader measurements without background interference from colored plastics. LabSupplies.com is an authorized Caplugs® Evergreen dealer and ships from USA inventory; the 10/Bag and 100/Case pack configurations support both routine per-bag ordering and case-quantity purchasing for high-throughput cell culture programs running large plate volumes weekly.
Frequently Asked Questions
Are these microplates tissue culture-treated?
No — part number 290-8124-01F is manufactured from untreated natural polystyrene. Untreated PS is suitable for moderately adherent cell lines using standard attachment protocols. For demanding primary cells that require enhanced surface attachment, tissue culture-treated (TC-treated) variants are available separately.
Are these plates sterile?
These plates are supplied non-sterile. They are intended for applications where the user applies their own sterility protocols or where sterility is not required, such as certain biochemical assays. If sterility is required for your cell culture workflow, confirm the appropriate product variant before ordering.
Will standard 15 mm round coverslips fit in the wells?
Yes — the approximately 15.5 mm inner well diameter is the standard 24-well dimension and accommodates 15 mm round glass coverslips, making these plates well-suited for confocal and widefield fluorescence imaging of adherent cells grown directly on coverslips in the well.
What is the maximum working volume per well?
The total well volume is 3.5 mL per well; a practical working volume of approximately 1–2.5 mL per well is recommended to allow adequate headspace for gas exchange and to prevent cross-contamination during plate handling. For virus plaque assays, standard overlay volumes in this format fall within that range.