1–2.5 mL Microplates, Flat Bottom, PS, Natural — 290-8124-01F | 10/Bag | Caplugs® Evergreen

24-Well Flat Bottom Microplates, 3.5 mL Well Volume, PS, Natural — 290-8124-01F | 10/Bag (100/Case)

Technical Narrative

The Caplugs® Evergreen is a 24-well flat-bottom Polystyrene (PS) microplate in the SBS/ANSI standard footprint — the standard format for cell culture applications requiring greater well volume and well diameter than the 96-well format supports. The 3.5 mL well volume and approximately 15.5 mm inner well diameter accommodate working volumes of 1–2.5 mL for primary cell culture, organoid embedding in Matrigel or basement membrane extract, and virus propagation assays requiring the large well surface area that virus plaque visualization demands. The 24-well format is the WHO-recommended and ATCC-standard format for plaque assay and TCID50 virus titration.

The flat bottom provides consistent optical imaging of cell monolayers and supports 15 mm round coverslip placement for high-magnification confocal and widefield fluorescence imaging of cells grown directly in the well. Natural PS is compatible with most standard cell attachment protocols without surface treatment for moderately adherent cell lines — for demanding primary cells, tissue culture-treated (TC-treated) variants are available. PS construction is compatible with standard cell culture reagents including DMEM, RPMI, MEM, DMSO (≤10%), and common fixatives at working concentrations.

At $137.89 per Bag of 10 — $1.3789 per plate — the delivers 24-well format cell culture capacity at bulk economics for programs running 20–100+ plates per week. The 10/Bag (100/Case) format supports both per-bag and case-quantity ordering for high-throughput programs.

Technical Specifications — Caplugs® Evergreen

Cell Culture & Assay Compatibility — 24-Well Flat Bottom

24-well flat-bottom plates are the standard format for cell culture scale-up and virus-based assays requiring more well volume than 96-well plates. Key applications:

Working volume is typically 1–2.5 mL — avoid exceeding 3 mL to prevent cross-contamination during plate handling. For coverslip-based imaging, 15 mm round coverslips seat flat in standard 24-well wells.

Chemical Resistance — Polystyrene (PS)

PS provides excellent aqueous reagent compatibility for cell culture and assay applications.

Laboratory Applications

The serves virology labs running plaque assay and TCID50 virus titration; cell biology programs culturing primary neurons, fibroblasts, keratinocytes, and epithelial cells at the 24-well scale; CRISPR functional screens using arrayed gRNA delivery; transfection and transduction scale-up experiments; organoid culture in Matrigel and BME; drug dose-response IC50/EC50 assays requiring sufficient cell yield for downstream Western blot, qPCR, and flow cytometry; and bacterial and yeast culture scale-up from 96-well hits.

U.S. Manufacturing, Quality & Institutional Procurement

Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.

Frequently Asked Questions — Caplugs® Evergreen

Is the 24-well format the standard for plaque assay (PFU) and TCID50?

Yes. 24-well flat-bottom PS plates are the standard format for plaque-forming unit (PFU) assay and TCID50 virus titration per WHO and ATCC standard protocols. The large well surface area (~1.9 cm²) supports a confluent monolayer of susceptible cells with sufficient area to score individual viral plaques, and the flat bottom allows uniform agarose or methylcellulose overlay application for plaque isolation. The 24-well format is preferred over 6-well (larger, more cells needed) and 96-well (too small for reliable plaque counting) for most standard virus titration protocols.

Can I use 15 mm coverslips in the 24-well plate for imaging?

Yes. Standard 15 mm (No. 1 or No. 1.5) round glass coverslips seat flat in the well bottom of standard 24-well PS plates. Sterilize coverslips before placing in wells, seed cells directly on the coverslip surface, and mount for confocal or widefield fluorescence imaging after fixation and staining. The flat PS bottom allows the coverslip to lie flat without tilting for consistent imaging across the coverslip surface.

Is the plate compatible with standard 24-well plate lids?

Yes. The uses the standard SBS/ANSI 24-well footprint and is compatible with the Caplugs® Evergreen 290-8125-01L 24-well PS lid, as well as standard 24-well lids from other manufacturers in SBS standard format. Always validate lid-to-plate fit with a sample pair before large-volume purchase.