96-Well Flat Bottom Microplates, 0.37 mL — Polystyrene, Natural, Non-Sterile, 10/Box (290-8115-01F)
These 96-well flat bottom polystyrene microplates deliver the uniform optical path length required for quantitative absorbance, fluorescence intensity, and luminescence plate-reader assays, with a 0.37 mL per-well volume that accommodates standard reagent addition sequences across ELISA, cell viability, enzyme kinetics, and nucleic acid quantification workflows. The natural (clear) polystyrene construction transmits light for transmission-mode absorbance reads and supports bottom-read fluorescence intensity, making these plates the standard format for general-purpose plate-reader assays. Supplied non-sterile in a 10/Box (100/Case) format, the 290-8115-01F conforms to the SBS/ANSI standard footprint and integrates directly with all major plate readers, liquid handlers, and robotic automation systems.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 290-8115-01F |
| Well Format | 96-Well |
| Footprint Standard | SBS/ANSI (127.76 × 85.47 mm) |
| Bottom Geometry | Flat Bottom |
| Well Volume | 0.37 mL |
| Material | Polystyrene (PS) |
| Color | Natural (Clear) |
| Sterility | Non-Sterile |
| Pack Size | 10/Box (100/Case) |
| BPA / Phthalates | BPA-Free; No Phthalate Plasticizers |
| Country of Origin | USA (California) |
Applications
- Colorimetric ELISA (HRP/TMB, AP/pNPP): Flat bottom geometry ensures consistent optical path length for OD450, OD405, and OD490 absorbance reads across the full plate.
- Cell Proliferation Assays (MTT, MTS, WST-1): Adherent cells attach to the flat polystyrene surface; absorbance is read at OD570/OD630 for viability quantification.
- DNA/RNA Quantification (PicoGreen, RiboGreen): Natural PS supports bottom-read fluorescence intensity for fluorescent dye-based nucleic acid quantification.
- Enzyme Kinetics / Substrate Consumption: Flat bottom maintains path length consistency during continuous absorbance monitoring over time.
- Protein Quantification (BCA, Bradford): Standard flat-bottom format for 96-well protein assay protocols.
- Luminescence Assays (Luciferase, ATP): Compatible with luminescence detection; laboratories requiring maximum signal enhancement should consider the companion white-well plate (290-8027-W1F) for 2–5× signal improvement.
- High-Content Screening / Plate Imaging: Flat bottom geometry is required for most plate imaging objectives in phenotypic cell assays.
Compatibility
Because these microplates conform to the SBS/ANSI standard footprint (127.76 × 85.47 mm), they seat correctly in all major plate readers — including SpectraMax®, PHERAstar®, Synergy™, Infinite®, and EnVision® — without adapter modifications. The same standardized geometry ensures reliable transport on automated liquid handlers such as Hamilton® and Tecan®, Bravo® platforms, plate washers, and robotic gripper arm systems, making the 290-8115-01F a drop-in fit for both manual and fully automated assay workflows.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers (PBS, TBS, HEPES, carbonate) | Excellent |
| Culture media and serum-containing solutions | Excellent |
| Dilute acids and bases (assay pH range) | Good |
| Standard ELISA detection reagents (TMB, pNPP, OPD) | Excellent |
| Ethanol (>70%) / isopropanol | Not recommended — PS swells |
| Acetone / chloroform / aromatic solvents | Not compatible — PS dissolves |
| Autoclaving | Not compatible — PS is not autoclavable |
Quality & Procurement
Made in USA (California), these Caplugs® Evergreen microplates are manufactured from BPA-free, phthalate-free polystyrene resin. The SBS/ANSI-compliant molding ensures plate-to-plate dimensional consistency critical for automated liquid handling and quantitative plate-reader assays. LabSupplies.com is an authorized Caplugs® Evergreen dealer stocking both the 10/Box and 100/Case formats and ships from USA inventory; tiered case pricing is available for laboratories running high plate volumes each week.
Frequently Asked Questions
Are these microplates sterile?
No — they are supplied non-sterile. For applications that require sterility, such as primary cell culture, a sterile-packaged flat bottom plate should be selected instead. For most plate-reader assays — ELISA, BCA, Bradford, enzyme kinetics — non-sterile plates are the standard and preferred format.
Can I use these plates for luminescence assays?
Yes, natural polystyrene is compatible with luminescence detection; however, the clear well walls do not reflect emitted light back toward the detector. If your assay requires maximum luminescence signal sensitivity, consider the white-well companion plate (290-8027-W1F), which can deliver 2–5× signal enhancement over natural PS.
Will these plates fit my automated liquid handler and plate reader without an adapter?
Yes — the SBS/ANSI standard footprint (127.76 × 85.47 mm) is the universal microplate format recognized by all major plate reader and liquid handler manufacturers, including SpectraMax, PHERAstar, Synergy, Hamilton, Tecan, and Bravo platforms, so no adapter or workaround is needed.
What is the working volume per well, and does it matter for my assay?
The maximum well volume is 0.37 mL; in practice, most assay protocols use 50–80% of maximum capacity — roughly 185–300 µL — to avoid cross-contamination from splashing during pipetting or shaking. Staying within that working range also helps maintain the meniscus geometry needed for accurate flat-bottom absorbance reads.