Caplugs® Evergreen
0.32 mL Microplates, Round Bottom, PP, Natural — 290-8353-03R | 10/Box | Caplugs® Evergreen
0.32 mL Microplates, Round Bottom, PP, Natural — 290-8353-03R | 10/Box | Caplugs® Evergreen
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96-Well Round (U-Bottom) Microplates, 0.32 mL Well Volume, PP, Natural — 290-8353-03R | 10/Box (100/Case)
| Quick Reference — 290-8353-03R | |
|---|---|
| Well Format | 96-well SBS/ANSI |
| Bottom | Round (U-Bottom) |
| Well Volume | 0.32 mL |
| Material | Polypropylene (PP) |
| Color | Natural |
| DMSO Compat. | ✓ All concentrations |
| Autoclave | ✓ Compatible |
| vs PS U-bottom | PP: DMSO + autoclave; PS (290-8117-01R): standard aqueous |
| Pack Size | 10/Box (100/Case) |
| Price | $120.33 / Box ($1.2033 / plate) |
Technical Narrative
The Caplugs® Evergreen is a 96-well PP U-bottom microplate — the DMSO-tolerant and autoclave-compatible upgrade from the PS U-bottom (290-8117-01R) for suspension cell culture and agglutination assays involving DMSO compound delivery or requiring autoclave pre-sterilization. PP provides full DMSO compatibility at all concentrations, where PS U-bottom plates can stress-crack under high DMSO compound formulations in drug discovery cell-based assays. The U-bottom geometry allows suspension cells and particles to settle uniformly by gravity for consistent supernatant aspiration and re-suspension across all 96 wells.
PP is autoclave-compatible at 121°C — enabling pre-sterilization of U-bottom plates for cell culture applications requiring sterile plate supply. PP's broad resistance to alcohols, detergents, and organic solvents makes it the U-bottom format for HAI and agglutination assays using buffer formulations containing organic co-solvents, preservatives, or high-concentration detergents that would stress PS plate geometry.
At $120.33 per Box of 10 — $1.2033 per plate — the PP U-bottom carries a modest premium over the PS variant (290-8117-01R, $120.33/box) justified when DMSO or autoclave requirements are present.
Technical Specifications — Caplugs® Evergreen
| Specification | Value |
|---|---|
| Part Number | |
| Caplugs Item # | 290835303RE1 |
| Well Format | 96-well SBS/ANSI |
| Bottom | Round (U-Bottom) |
| Well Volume | 0.32 mL |
| Material | Polypropylene (PP) |
| Color | Natural |
| DMSO Compat. | ✓ All concentrations — key PP advantage |
| Autoclave | ✓ Compatible (121°C / 15 psi) |
| vs PS variant | 290-8117-01R (PS): standard aqueous; this PP: DMSO + autoclave |
| Pack Size | 10/Box (100/Case) |
| BPA/Phthalate | BPA-free; no phthalate plasticizers |
| Country of Origin | Made in U.S.A. (California) |
PP U-Bottom Workflow Compatibility — DMSO & Autoclave Advantage
PP U-bottom plates extend round-bottom suspension cell culture and agglutination assays to chemically demanding workflows:
| Application | Assay / Use | Notes |
|---|---|---|
| Suspension cell culture (DMSO) | Lymphocytes, PBMCs in DMSO compound media | PP tolerates DMSO; round bottom for uniform cell settling |
| HAI / agglutination (harsh media) | Agglutination assays with organic-solvent additives | PP resists solvents that stress PS at U-bottom well |
| Compound-treated suspension | Drug treatment of suspension cell lines | PP DMSO compatibility + round bottom cell settling |
| Bead assay (DMSO matrix) | Magnetic bead assay in DMSO-containing buffer | PP resists DMSO; round bottom pellets beads |
| Hybridoma (autoclave-sterile) | Hybridoma culture in autoclave-sterilized plates | PP autoclave-compatible; round bottom for suspension culture |
| Cytokine bead array (PP) | Multiplex bead FI in DMSO media formulations | PP broader DMSO tolerance for compound-treated suspension assays |
| Serial dilution (organic solvent) | Dilution series with DMSO or alcoholic diluents | PP chemical resistance for non-aqueous dilution matrices |
| Immunoprecipitation (DMSO buffer) | IP bead assay with DMSO-containing lysis buffer | PP resists RIPA/IP buffers + DMSO co-solvent |
PP is translucent — slightly less clear than PS, sufficient for visual cell assessment. For standard aqueous suspension culture and HAI without DMSO, PS U-bottom (290-8117-01R) is the cost-effective choice.
Chemical Resistance — Polypropylene (PP)
PP: full DMSO compatibility and autoclave sterilization — key advantages over PS U-bottom.
| Reagent / Condition | Compatibility |
|---|---|
| DMSO (all concentrations) | ✓ Excellent — key advantage over PS |
| Aqueous buffers | ✓ Excellent |
| Short-chain alcohols | ✓ Excellent |
| Dilute acids / alkalies | ✓ Excellent |
| Chloroform / hexane (brief) | ✓ Good |
| Detergents (RIPA, NP-40) | ✓ Good |
| Autoclave (121°C / 15 psi) | ✓ Compatible |
| Storage at -20°C / -80°C | ✓ Suitable |
Laboratory Applications
Serves drug discovery suspension cell-based assay programs delivering DMSO compound formulations; immunology labs running HAI assays with chemically complex buffers; hybridoma culture programs requiring autoclave-sterilized plates; cytokine bead array programs in DMSO-containing media; and any U-bottom application where PS DMSO tolerance is a documented limitation.
U.S. Manufacturing, Quality & Institutional Procurement
Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.
Frequently Asked Questions — Caplugs® Evergreen
When should I choose PP U-bottom over PS U-bottom (290-8117-01R)?
Choose PP when: (1) DMSO is present at >0.5% working concentration; (2) Autoclave sterilization is required before use; (3) Organic co-solvents, concentrated alcohols, or harsh detergents are in the assay buffer. PS U-bottom (290-8117-01R) is the cost-effective choice for standard aqueous suspension culture, HAI agglutination, and PBMC culture without DMSO.
Is the PP U-bottom plate compatible with HAI hemagglutination assays?
Yes. PP U-bottom plates support the same RBC settling and button-pattern scoring as the PS U-bottom format — the round geometry concentrates RBCs at the well center for clear agglutination vs button distinction. PP is specifically advantageous when the HAI assay buffer includes preservatives, co-solvents, or detergents that would stress PS plate geometry over multiple plate uses.
Are the PP U-bottom plates BPA-free and autoclave-compatible?
Yes to both. Virgin PP, no BPA, no phthalates. Autoclave-compatible at 121°C / 15 psi.
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