Caplugs® Evergreen
Microplates, PET, Natural — 290-8121-010 | 10/Box | Caplugs® Evergreen
Microplates, PET, Natural — 290-8121-010 | 10/Box | Caplugs® Evergreen
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96-Well Open Bottom Microplates, PS, Natural — 290-8121-010 | 10/Box (100/Case)
| Quick Reference — 290-8121-010 | |
|---|---|
| Well Format | 96-well SBS/ANSI standard footprint |
| Bottom | Open (no well floor — bottom of wells is open) |
| Material | Polystyrene (PS) |
| Color | Natural (transparent frame) |
| Primary Use | Membrane insert, SPR, electrode contact, TIRF imaging |
| Sterility | Non-Sterile |
| Pack Size | 10/Box (100/Case) |
| Price | $112.61 / Box ($1.1261 / plate) |
Technical Narrative
The Caplugs® Evergreen is a 96-well open-bottom Polystyrene (PS) plate frame — the platform format for applications that require physical, optical, or electrical access to the underside of each well in a standard SBS 96-well footprint. Unlike standard microplates where the well floor seals each well from below, the open-bottom plate has no well floors — each of the 96 positions is open at the bottom, enabling direct contact between well contents and membrane filters, sensor chip surfaces, electrode arrays, or custom functionalized surfaces bonded to the plate underside. This is the enabling platform format for Boyden chamber cell migration assays, surface plasmon resonance plate-format detection, Meso Scale Discovery (MSD) electrochemiluminescence assays, and total internal reflection fluorescence (TIRF) imaging.
The PS frame maintains SBS/ANSI footprint compatibility for all standard liquid handlers, plate readers, and plate washers that need to address wells by position — providing open-bottom flexibility within the standard automation infrastructure. The plate can be bonded with porous membranes (PET, polycarbonate, PTFE) for cell migration and invasion assays, coated with gold sensor film for SPR detection, paired with electrode arrays for electrochemical detection, or used as a microfluidic interface platform where standard solid-bottom plates cannot provide the necessary bottom access.
At $112.61 per Box of 10 — $1.1261 per plate — the delivers open-bottom platform format at economics suited to high-throughput assay programs requiring custom surface or membrane integration at 96-well scale.
Technical Specifications — Caplugs® Evergreen
| Specification | Value |
|---|---|
| Part Number | |
| Caplugs Item # | 2908121010E1 |
| Well Format | 96-well SBS/ANSI standard footprint |
| Bottom | Open — no well floor; bottom of all 96 wells is open |
| Material | Polystyrene (PS) |
| Color | Natural |
| Membrane Compat. | Accepts standard 96-well membrane inserts and filter bases |
| SPR Compat. | Open bottom allows sensor chip or functionalized surface contact |
| Electrode Compat. | Open bottom required for MSD/Meso Scale Detection |
| Sterility | Non-Sterile |
| Pack Size | 10/Box (100/Case) |
| Application | Transwell, Boyden chamber, SPR, MSD, TIRF, custom surfaces |
| BPA / Phthalate | BPA-free; no phthalate plasticizers |
| Country of Origin | Made in U.S.A. (California) |
Open Bottom Platform Applications —
The 96-well open-bottom plate provides a reusable SBS-format frame for applications requiring physical access to the bottom of each well:
| Application | Assay / Use | Notes |
|---|---|---|
| Membrane filter inserts | Cell migration (Boyden chamber), invasion | Open bottom accepts membrane inserts for transwell-style assays |
| Surface plasmon resonance (SPR) | Biacore, ProteOn plate-based SPR | Open bottom allows sensor chip or functionalized surface contact |
| Electrochemistry assays | MSD Sector Imager, electrode-based detection | Open bottom required for direct electrode contact with well contents |
| Custom surface attachment | Antibody coating, bead immobilization | Open bottom enables surface functionalization from below |
| TIRF / FRET imaging | Total internal reflection fluorescence | Open bottom allows objective-contact imaging modes |
| Patch clamp assays | Ion channel functional assays | Open bottom accommodates patch clamp electrode access |
| Cell migration / scratch assay | Wound healing assay with bottom access | Open bottom enables underside imaging without plate inversion |
| Microfluidic integration | Microfluidic chip connection to wells | Open bottom enables microfluidic channel coupling from plate underside |
The open-bottom plate is a frame — it has no inherent containment without a membrane, filter, or custom surface attached at the base. Always pair with an appropriate membrane insert or bottom layer for your specific application before use.
Chemical Resistance — Polystyrene (PS)
PS frame provides excellent resistance to aqueous assay reagents.
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers (PBS, TBS, HEPES) | ✓ Excellent |
| Cell culture media | ✓ Excellent |
| DMSO (≤10%) | ✓ Good |
| Short-chain alcohols (brief) | ✓ Good |
| Dilute acids / alkalies (<10%) | ✓ Good |
| Formalin (4%, fixation) | ✓ Good |
| Acetone | ✗ Avoid |
| Autoclave | ✗ Not compatible |
Laboratory Applications
The serves cell biology programs running Boyden chamber cell migration and invasion assays; immunology labs running neutrophil and lymphocyte chemotaxis; biosensor and surface chemistry programs developing SPR and electrochemical plate-format detection; MSD/Meso Scale Discovery electrochemiluminescence assay programs; advanced fluorescence imaging programs using TIRF and FRET requiring objective-contact access to well bottoms; patch clamp and ion channel programs; microfluidic integration platforms; and any application requiring physical or optical access to the well bottom within an SBS 96-well footprint.
U.S. Manufacturing, Quality & Institutional Procurement
Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.
Frequently Asked Questions — Caplugs® Evergreen
What is an open-bottom microplate used for?
An open-bottom plate has no well floors — each well position is open at the bottom. This enables: (1) Membrane filter inserts for Boyden chamber cell migration and invasion assays; (2) Sensor chip contact for surface plasmon resonance (SPR) plate-format assays; (3) Electrode array contact for MSD Meso Scale electrochemiluminescence; (4) Objective-contact fluorescence imaging modes (TIRF, FRET); (5) Custom surface functionalization from the well bottom; (6) Microfluidic channel coupling. The plate frame maintains SBS footprint for standard automation compatibility.
Does the open-bottom plate contain liquid without a membrane?
No. The has no well floors — it is a frame only. Without a membrane, filter, or bonded surface covering the open well bottoms, liquid will pass through immediately. The plate must always be paired with an appropriate bottom layer (porous membrane, solid surface, sensor chip, custom coating) before loading any liquid into the wells.
Is the open-bottom plate compatible with standard plate readers and liquid handlers?
Yes — for detection modes that don't require a well floor. The SBS/ANSI footprint ensures compatibility with standard robotic liquid handlers for well addressing and dispensing. For plate reader detection, the reader must be configured for the specific detection mode of your membrane/surface application (e.g., MSD Sector Imager for electrochemiluminescence, TIRF-capable microscope for TIRF imaging). Standard absorbance reads cannot be performed without a well floor.
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