0.32 mL Microplates, Round Bottom, PS, Natural — 290-8117-01R | 10/Box | Caplugs® Evergreen

96-Well Round (U-Bottom) Bottom Microplates, 0.32 mL Well Volume, PS, Natural — 290-8117-01R | 10/Box (100/Case)

Technical Narrative

The Caplugs® Evergreen is a 96-well round (u-bottom) bottom Polystyrene (PS) microplate in natural for suspension cell culture, cell-based aggregation assays, and wash-based immunoassays requiring cell pelleting by gravity. The U-bottom geometry allows cells and particles to settle by gravity into a uniform central pellet — enabling consistent supernatant aspiration and re-suspension without the cell loss that occurs with flat or V-bottom wells during aspiration. Natural (clear) PS for suspension cell culture and visual agglutination reads. The U-bottom geometry is the defining feature — color choice is secondary to bottom geometry for these applications.

All 96-well Caplugs® Evergreen microplates conform to the SBS/ANSI microplate standard footprint (127.76 × 85.47 mm), ensuring compatibility with all standard plate readers (SpectraMax, PHERAstar, Synergy, Infinite, EnVision), automated liquid handlers (Hamilton, Tecan, Bravo), plate washers, and robotic gripper arm systems. The 0.32 mL well volume accommodates standard assay reagent addition sequences across the primary applications for round (u-bottom) format plates. PS construction is compatible with all standard aqueous assay buffers, culture media, and detection reagents.

At $120.33 per Box of 10 — $1.2033 per plate — the delivers round (u-bottom) PS microplate economics for programs running 10–200+ plates per week. The 10/Box (100/Case) format supports both per-box and case-quantity ordering.

Technical Specifications — Caplugs® Evergreen

Assay & Application Compatibility — Round (U-Bottom) Bottom, Natural

Round (U-Bottom) bottom PS natural plates are the standard format for suspension cell culture, cell-based aggregation assays, and wash-based immunoassays requiring cell pelleting by gravity. Compatible workflows:

Natural (clear) PS for suspension cell culture and visual agglutination reads. The U-bottom geometry is the defining feature — color choice is secondary to bottom geometry for these applications.

Chemical Resistance — Polystyrene (PS)

PS provides excellent resistance to aqueous assay reagents. Not autoclave-compatible. Avoid acetone and chlorinated solvents.

Laboratory Applications

The U-bottom plate serves immunology and hematology labs running HAI hemagglutination inhibition and viral neutralization assays; cell biology programs culturing suspension lymphocytes, PBMCs, and hybridomas; microbiology labs running MIC broth microdilution; cytokine bead array and multiplex bead assay programs; immunoprecipitation workflows; and any application requiring cells or particles to settle uniformly by gravity.

U.S. Manufacturing, Quality & Institutional Procurement

Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.

Frequently Asked Questions — Caplugs® Evergreen

Why use U-bottom instead of flat-bottom for suspension cells?

In a flat-bottom well, suspension cells distribute across the entire flat floor — making complete aspiration difficult without disturbing the cell layer. The U-bottom geometry allows cells to settle by gravity into a uniform central pellet at the well bottom, enabling clean supernatant aspiration and minimal cell loss during wash steps. This is the defining requirement for HAI assays (where RBC pellet button vs streaming is the read), hybridoma culture, and PBMC-based assays.

Is the plate the standard format for HAI assays?

Yes. Round (U-bottom) 96-well PS plates are the WHO-specified format for hemagglutination inhibition (HAI) and viral neutralization assays. The U-bottom geometry allows red blood cells to settle into a uniform button pattern that can be visually scored (button = inhibition; streaming/shield = hemagglutination). Flat-bottom plates produce irregular RBC distribution that is difficult to score reliably.

Is the compatible with automated plate readers for cell-based assays?

Yes, with the caveat that U-bottom geometry affects path length for absorbance reads. U-bottom plates are primarily used for visual agglutination scoring, luminescence, and fluorescence intensity detection modes — not for quantitative absorbance ELISA where flat-bottom is required for accurate path length. The SBS/ANSI footprint is compatible with all standard plate readers for luminescence and fluorescence detection.