Caplugs® Evergreen
0.32 mL Microplates, Round Bottom, PS, White — 290-8029-W1R | 9/Box | Caplugs® Evergreen
0.32 mL Microplates, Round Bottom, PS, White — 290-8029-W1R | 9/Box | Caplugs® Evergreen
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96-Well Round Bottom Microplates, 0.32 mL Well Volume, PS, White — 290-8029-W1R | 9/Box (90/Case)
| Quick Reference — 290-8029-W1R | |
|---|---|
| Well Format | 96-well SBS/ANSI standard footprint |
| Bottom Geometry | Round Bottom |
| Well Volume | 0.32 mL |
| Material | Polystyrene (PS) |
| Color | White |
| Primary Use | Suspension cell-based luminescence assays and agglutination assays requiring both round-bottom cell/particle behavior and enhanced luminescence signal |
| Sterility | Non-Sterile |
| Pack Size | 9/Box (90/Case) |
| Price | $127.63 / Box ($1.4181 / plate) |
Technical Narrative
The Caplugs® Evergreen is a 96-well round bottom Polystyrene (PS) microplate in white for suspension cell-based luminescence assays and agglutination assays requiring both round-bottom cell/particle behavior and enhanced luminescence signal. The white round bottom combines two properties that suspension luminescence assays require simultaneously: round-bottom well geometry for suspension cell culture, and white reflective PS walls that maximize luminescence signal from luciferase and ATP bioluminescence reporter systems. White PS is specified for luminescence-based assays in suspension cell culture — combining the round-bottom geometry needed for suspension cells with the reflective white surface that maximizes luminescence signal detection.
All 96-well Caplugs® Evergreen microplates conform to the SBS/ANSI microplate standard footprint (127.76 × 85.47 mm), ensuring compatibility with all standard plate readers (SpectraMax, PHERAstar, Synergy, Infinite, EnVision), automated liquid handlers (Hamilton, Tecan, Bravo), plate washers, and robotic gripper arm systems. The 0.32 mL well volume accommodates standard assay reagent addition sequences across the primary applications for round format plates. PS construction is compatible with all standard aqueous assay buffers, culture media, and detection reagents.
At $127.63 per Box of 9 — $1.4181 per plate — the delivers round PS microplate economics for programs running 10–200+ plates per week. The 9/Box (90/Case) format supports both per-box and case-quantity ordering.
Technical Specifications — Caplugs® Evergreen
| Specification | Value |
|---|---|
| Part Number | |
| Caplugs Item # | 2908029W1RE1 |
| Well Format | 96-well SBS/ANSI standard footprint |
| Bottom Geometry | Round Bottom |
| Well Volume | 0.32 mL (working volume typically 50–80% of max) |
| Material | Polystyrene (PS) |
| Color | White |
| Sterility | Non-Sterile |
| Pack Size | 9/Box (90/Case) |
| Application | Suspension cell-based luminescence assays and agglutination assays requiring both round-bottom cell/particle behavior and enhanced luminescence signal |
| BPA / Phthalate | BPA-free; no phthalate plasticizers |
| Country of Origin | Made in U.S.A. (California) |
Assay & Application Compatibility — Round Bottom, White
Round bottom PS white plates are the standard format for suspension cell-based luminescence assays and agglutination assays requiring both round-bottom cell/particle behavior and enhanced luminescence signal. Compatible workflows:
| Application | Assay / Use | Notes |
|---|---|---|
| Cell viability (luminescence) | CellTiter-Glo, CTG 2.0, ATPlite | White round-bottom: max luminescence from suspension cell pellet |
| Suspension cell reporter assays | Luciferase reporter in suspension cells | White enhances luciferase signal; round bottom for suspension culture |
| HAI / hemagglutination (enhanced read) | Flu virus titer, VN assay | Round bottom for uniform RBC distribution; white for optical read |
| Hybridoma luminescence screening | Luminescent reporter hybridoma clones | Combined benefit of U-bottom culture and white luminescence signal |
| Bioluminescence imaging | Whole-well bioluminescence detection | White wells maximize photon capture for imaging-compatible luminometers |
| ATP bioluminescence (suspension) | Bacterial ATP, metabolic activity | White round-bottom: complete suspension culture + luminescence read |
| ADCC / CDC cytotoxicity | Antibody-dependent cellular cytotoxicity | Round bottom for effector/target cell co-culture with luminescence readout |
| Cell-based NF-κB assays | Suspension Jurkat reporter cells | White round-bottom for dual suspension culture + luminescence read |
White PS is specified for luminescence-based assays in suspension cell culture — combining the round-bottom geometry needed for suspension cells with the reflective white surface that maximizes luminescence signal detection.
Chemical Resistance — Polystyrene (PS)
PS provides excellent resistance to aqueous assay reagents. Not autoclave-compatible. Avoid acetone and chlorinated solvents.
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers (PBS, TBS, HEPES) | ✓ Excellent |
| Cell culture media (DMEM, RPMI) | ✓ Excellent |
| DMSO (≤10%) | ✓ Good |
| Short-chain alcohols (brief) | ✓ Good |
| Dilute acids / alkalies (<10%) | ✓ Good |
| Formalin (4%, fixation step) | ✓ Good |
| Acetone | ✗ Avoid |
| Chlorinated solvents | ✗ Avoid |
| Autoclave (121°C) | ✗ Not compatible |
Laboratory Applications
The white round-bottom plate serves cell biology programs running luciferase reporter assays in suspension cells; virology and immunology labs combining HAI/agglutination with luminescence detection; drug discovery programs using ATP bioluminescence in suspension cells; and any application requiring both U-bottom suspension culture geometry and white-plate luminescence signal enhancement.
U.S. Manufacturing, Quality & Institutional Procurement
Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.
Frequently Asked Questions — Caplugs® Evergreen
Why use a white round-bottom plate instead of a white flat-bottom for luminescence?
White flat-bottom (290-8027-W1F) is optimal for adherent cell luminescence and absorbance assays where cells attach to the well floor. White round-bottom is designed for suspension cell luminescence — the U-bottom geometry allows suspension cells to settle into a uniform pellet, while the white well walls maximize photon reflection for the luciferase or ATP bioluminescence read. Choose round-bottom when your cells are in suspension (Jurkat, primary lymphocytes, bacteria); choose flat-bottom when cells are adherent.
Is the suitable for CellTiter-Glo and similar luminescent cell viability assays?
Yes. White round-bottom plates are the recommended format for CellTiter-Glo (CTG), CellTiter-Glo 2.0, and ATPlite luminescent cell viability assays with suspension cells. The white surface enhances ATP bioluminescence signal 2–5x versus clear plates, and the round bottom concentrates suspension cells for uniform CTG reagent mixing. For adherent cells, white flat-bottom (290-8027-W1F) is preferred.
Is the compatible with standard luminometers?
Yes. The SBS/ANSI standard footprint is compatible with all standard luminometers including SpectraMax L, Centro XS3, GloMax, and PHERAstar FSX in luminescence mode. White plates are optimized for top-read luminescence — the plate reader reads from above the well, and white walls reflect all photons toward the top detector.
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