0.32 mL Round-Bottom 96-Well Microplate with Lid, Sterile — PS, Natural, 100/Box (222-8032-01R)
These sterile 96-well polystyrene microplates feature a U-shaped round-bottom well geometry that allows suspension cells to settle into a gentle cluster at the bottom center, making them the standard choice for lymphocyte culture, mixed lymphocyte reactions, NK cell cytotoxicity assays, and ELISpot precursor workflows. Each plate holds a maximum of 0.32 mL per well and ships individually wrapped with its matching lid — sterility is maintained until the wrapper is opened at the biosafety cabinet. Supplied 100 plates per box, part number 222-8032-01R covers high-throughput immunology labs and cell biology groups that need individually packed, assay-ready plates at scale.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-8032-01R |
| Format | 96-well microplate with matching lid |
| Well Bottom Style | Round (U-shaped) |
| Well Volume (max) | 0.32 mL per well |
| Material | Polystyrene (PS) |
| Color | Natural |
| Lid | Included — individually packed with plate |
| Sterility | Sterile |
| Pack Size | 100/Box, individually packed |
| Footprint | SBS/ANSI standard |
| BPA / Phthalate / Latex | BPA-free, phthalate-free, latex-free |
| Country of Origin | USA |
Applications
- Suspension lymphocyte culture: The U-shaped well bottom allows non-adherent cells to form a loose central cluster without the harsh pellet concentration associated with V-bottom plates, maintaining cell-cell contact for proliferation and cytokine stimulation assays.
- Mixed lymphocyte reaction (MLR): Allogeneic cell co-culture in round-bottom wells promotes even cell contact across the effector-to-target population, a prerequisite for accurate proliferation readouts.
- NK cell cytotoxicity assays: Round-bottom geometry keeps target cells clustered for consistent effector-to-target ratios at each well position.
- ELISpot precursor culture: Pre-stimulation of antigen-specific T cells in suspension before transfer to ELISpot membrane plates.
- T-cell activation assays: Anti-CD3/CD28 or superantigen stimulation where T-cell clustering enhances activation signal.
- Hybridoma cloning and antibody production: 96-well round-bottom format supports single-cell or low-density cloning workflows.
- Plate reader detection: Optically clear polystyrene with low autofluorescence supports absorbance (400–750 nm) and fluorescence-based endpoint reads on standard SBS/ANSI-footprint readers.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers, saline, PBS | Excellent |
| Cell culture media (RPMI, DMEM, etc.) | Excellent |
| Dilute acids and bases | Good — short-term contact |
| Ethanol (<70%) | Limited — brief surface contact only |
| Acetone | Not recommended — dissolves PS |
| Chlorinated solvents (chloroform, DCM) | Not recommended |
| Aromatic solvents (toluene, xylene) | Not recommended |
| Autoclaving | Not compatible — PS is not autoclavable |
Quality & Procurement
Made in USA, these Caplugs® Evergreen microplates are produced from FDA-compliant, BPA-free, phthalate-free, and latex-free polystyrene under ISO 9001 quality management. Individual plate-and-lid packaging preserves sterility until point of use, eliminating the need for pre-use UV treatment or additional decontamination steps. LabSupplies.com is an authorized Caplugs® Evergreen dealer and ships from USA inventory, typically same business day for stocking items, with tiered pricing available for high-volume institutional purchasers and grant-funded laboratories.
Frequently Asked Questions
What is the difference between round-bottom (U-bottom) and V-bottom microplates for cell culture?
Round-bottom (U-shaped) wells allow suspension cells such as lymphocytes to settle into a loose, gently curved cluster at the center of the well. This promotes cell-cell contact without the tight, potentially stressful pellet that forms in V-bottom wells. U-bottom plates are preferred for proliferation assays, MLR, and NK cytotoxicity where cell viability and even distribution matter. V-bottom plates are better suited to cell harvesting or washing steps where tight pelleting is desirable.
Is each plate individually sterile-wrapped, and how should it be opened?
Yes — every plate in the 100/box pack is individually wrapped with its matching lid to maintain sterility from the factory through to your workflow. Open each wrapper inside a biosafety cabinet immediately before use to preserve the sterile field; do not pre-open plates and store them outside the wrapper.
Are these plates compatible with standard plate readers and automated liquid handlers?
The SBS/ANSI standard footprint ensures compatibility with the vast majority of microplate readers, washers, and automated liquid-handling platforms. The optically clear polystyrene supports absorbance reads from approximately 400–750 nm and fluorescence-based detection with low background autofluorescence.
Are there other well-bottom formats available in this same plate series?
Yes — Caplugs® Evergreen offers companion plates in the same 96-well, sterile, individually packed format: a flat-bottom plate (222-8030-01F, 0.37 mL), a conical V-bottom plate (222-8031-01V, 0.30 mL), and a chamfered-bottom plate (222-8033-01C, 0.35 mL). Selecting the appropriate geometry for your assay type is straightforward when ordering the full series.