Caplugs® Evergreen
0.32 mL Microplates, Conical Bottom, PS, Natural — 222-8032-01R | 100/Box | Caplugs® Evergreen
0.32 mL Microplates, Conical Bottom, PS, Natural — 222-8032-01R | 100/Box | Caplugs® Evergreen
Couldn't load pickup availability
Quick Reference
| Part Number | 222-8032-01R |
|---|---|
| Format | 96-well microplate with matching lid |
| Well Bottom | Round |
| Well Geometry | U-shaped round well bottom — gentle curve for suspension cell culture and mixing |
| Well Volume | 0.32 mL max per well |
| Material | Polystyrene (PS) |
| Color | Natural |
| Lid | Included — individually packed plate + lid |
| Sterility | Sterile |
| Pack Size | 100/Box Individually Packed |
| Primary Use | Suspension lymphocyte culture, mixed lymphocyte reaction (MLR), ELISpot, NK cell assays |
| Origin | Made in USA |
| Price | $241.77/Box ($2.4177/plate) |
Technical Narrative
The Caplugs® Evergreen 222-8032-01R is a 96-Well Round Bottom PS Sterile Microplate with Lid, 0.32 mL per well, individually packed, 100/Box. The round (U-shaped) well bottom provides a gentle curved surface suited to suspension cell culture — lymphocytes and other non-adherent cells settle into a loose cluster at the bottom center of the round well, maintaining cell-cell contact for proliferation assays and cytokine stimulation while avoiding the harsh cell pellet concentration of a V-bottom. Round-bottom plates are standard for mixed lymphocyte reactions (MLR), NK cell cytotoxicity assays, ELISpot precursor cultures, and T-cell activation assays.
PS cell culture-grade polystyrene: surface-treated for enhanced cell attachment in adherent culture formats, optically clear for plate reader compatibility (400–750 nm), low autofluorescence for fluorescence-based detection. Each plate ships individually wrapped with its matching lid — sterility maintained until the individual wrapper is opened at the BSC. BPA-free, phthalate-free, FDA-compliant,
Procurement economics: $241.77/Box (100 plates), $2.4177/plate. 96-well set: 222-8030-01F (96-Well Flat Bottom 0.37 mL); 222-8031-01V (96-Well Conical Bottom 0.30 mL); 222-8033-01C (96-Well Chamfered Bottom 0.35 mL). LabSupplies.com ships from USA inventory, typically same business day.
Technical Specifications
| Part Number | |
|---|---|
| Caplugs Item # | |
| Format | 96-well microplate with matching lid |
| Well Bottom | Round |
| Well Geometry | U-shaped round well bottom — gentle curve for suspension cell culture and mixing |
| Well Volume | 0.32 mL max per well |
| Material | Polystyrene (PS) |
| Color | Natural |
| Lid | Included — individually packed with plate |
| Sterility | Sterile |
| Pack Size | 100/Box Individually Packed |
| Primary Application | Suspension lymphocyte culture, mixed lymphocyte reaction (MLR), ELISpot, NK cell assays |
| Plate Reader | Compatible — SBS/ANSI standard footprint |
| BPA / Phthalate | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
| Price | $241.77/Box ($2.4177/plate) |
96-Well Round Bottom Microplate Applications
The is compatible with the following assay and cell biology workflows.
| Application | Setting / Configuration | Notes |
|---|---|---|
| Suspension lymphocyte culture | Incubator, round-bottom 96-well | U-bottom: cells cluster gently without harsh pellet formation |
| Mixed lymphocyte reaction (MLR) | Allogeneic cell co-culture, 96-well | Round bottom: cell-cell contact for proliferation assay |
| NK cell cytotoxicity | E:T ratio assay, 96-well round bottom | U-bottom: target cell clustering for NK contact |
| ELISpot precursor culture | Pre-stimulation culture, round-bottom | Suspension culture for antigen-specific T-cell stimulation |
| T-cell activation assay | Anti-CD3/CD28 stimulation, 96-well | Round bottom: T-cell clustering for activation |
| Cell co-culture | Mixed cell population culture | U-bottom: cell-cell interaction without selective attachment |
| Antibody production hybridoma | 96-well round-bottom cloning | Round bottom: single-cell HAT selection culture |
Chemical Resistance — Polystyrene (PS) Cell Culture Microplate
Compatibility reference for PS at typical assay and cell culture conditions.
| Reagent / Condition | Compatibility | Notes |
|---|---|---|
| Aqueous buffers / saline | Excellent | PS compatible across pH 4–10. |
| Cell culture media (RPMI, DMEM) | Excellent | PS: standard cell culture material. |
| Formalin (10% NBF) | Excellent | Compatible for cell fixation. |
| Methanol (<100%) | Limited | Short-term compatible; avoid prolonged contact. |
| DMSO (<1%) | Good | Compatible at standard assay concentrations. |
| Detergents (Tween-20, Triton) | Good | Compatible at standard assay concentrations. |
| Acetone / chloroform | Not Compatible | Dissolves PS; do not use. |
| UV (<320 nm) | Not Transparent | PS opaque to UV; use UV-transparent plates for UV absorbance. |
Clinical & Research Laboratory Applications
Caplugs® Evergreen 96-well round (U-bottom) PS sterile microplates serve immunology, transplant medicine, and cell therapy laboratories performing suspension lymphocyte assays in the 96-well plate format. The mixed lymphocyte reaction (MLR) — used in transplant immunology for donor-recipient HLA compatibility assessment — requires a round-bottom plate where allogeneic lymphocytes co-culture in suspension with cell-to-cell contact maintained by the U-shaped well geometry. NK cell cytotoxicity assays (chromium release, flow cytometry-based), ELISpot precursor stimulation cultures, and antigen-specific T-cell proliferation assays also specify the round-bottom format.
U.S. Manufacturing, Quality & Institutional Procurement
Caplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.
LabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can request a written quote or speak with a product specialist directly from our sales engineering team.
Frequently Asked Questions
Are the 96-well microplates sterile?
Yes. Sterile — each plate individually packed with its matching lid, 100/Box.
What is the complete 96-well microplate bottom geometry set from Caplugs Evergreen?
Four bottom geometries: Flat (222-8030-01F, 0.37 mL), Conical V-bottom (222-8031-01V, 0.30 mL), Round U-bottom (, 0.32 mL), Chamfered (222-8033-01C, 0.35 mL). All are sterile PS with matching lids, individually packed 100/Box.
When should I use a round-bottom vs a V-bottom (222-8031-01V)?
Round U-bottom for suspension cell biology where gentle cell clustering is needed — MLR, NK cytotoxicity, T-cell activation, ELISpot precursor culture. V-bottom for agglutination assays and tight pellet concentration. The key difference is cell viability after the incubation: round-bottom cells are less stressed and more viable for downstream assays.
Share
