5.0 mL Round Bottom Test Tubes with Two-Position Snap Cap — PPC, Natural, Sterile, 12 × 75 mm
These 5.0 mL round bottom test tubes measure 12 × 75 mm and are constructed from natural Polypropylene Copolymer (PPC) — a flexible, impact-resistant resin well suited to centrifugation and repeated freeze-thaw cycles. Each tube ships individually packed and sterile, with a two-position snap cap that supports both vented and sealed operation. Carrying part number 222-2376-08I, they are a staple format for flow cytometry, cell sorting, hematology sample preparation, and serological assays where round bottom geometry is required for efficient pellet concentration.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-2376-08I |
| Volume | 5.0 mL |
| Dimensions | 12 × 75 mm |
| Bottom Style | Round Bottom |
| Cap Style | Two-Position Snap Cap (vented / sealed) |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| Sterility | Sterile |
| Pack Size | 1 / Individually Packed |
| BPA / Phthalate Free | Yes |
| Country of Origin | USA |
Applications
- Flow Cytometry & FACS: The 12 × 75 mm round bottom format is the standard geometry for flow cytometer sample probe access; natural PPC is compatible with fluorescent staining buffers and fixatives.
- Cell Sorting & Immunofluorescence: Round bottom concentrates cell pellets at the tube base after centrifugation, enabling efficient resuspension and minimal sample loss during immunostaining workflows.
- Hematology Sample Preparation: The 5.0 mL capacity and standard dimensions suit automated and manual hematology dilution and analysis protocols.
- Serological & Agglutination Assays: Round bottom geometry allows visible agglutination patterns to settle and read accurately without interference from tube corners.
- Specimen Collection & Transport: The two-position snap cap seals securely for transport and opens to a vented position for incubation, eliminating the need to swap closures between workflow steps.
- Freeze-Thaw Sample Storage: PPC flexibility and impact resistance support multiple freeze-thaw cycles without cracking, making these tubes suitable for biobanking and specimen archiving at low temperatures.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline | Excellent |
| Dilute acids | Excellent |
| Dilute bases | Excellent |
| Methanol | Compatible |
| Isopropanol | Compatible |
| Acetonitrile | Compatible |
| Aromatic solvents (e.g., toluene, xylene) | Limited — not recommended for prolonged contact |
Quality & Procurement
Made in USA at Caplugs® Evergreen ISO-certified facilities, these tubes are manufactured from virgin-grade, FDA-registered PPC resin that is BPA-free and phthalate-free. Lot-level quality controls ensure consistent tube geometry, wall thickness, and dimensional uniformity across every individually packed unit. LabSupplies.com is an authorized Caplugs® Evergreen dealer shipping from USA inventory.
Frequently Asked Questions
Are these tubes supplied sterile, and is sterility maintained in individual packaging?
Yes — each tube is individually packed and sterile. The sealed packaging preserves sterility from the point of manufacture through point-of-use opening, making these tubes ready for cell culture, specimen collection, and other contamination-sensitive workflows without additional sterilization steps.
What does the two-position snap cap do, and can I switch between positions mid-protocol?
The two-position snap cap offers a vented configuration for gas exchange during cell incubation and a fully sealed configuration for containment during centrifugation or transport. The positions can be toggled by hand mid-protocol without tools, eliminating the need to swap or store separate closures.
Why is a round bottom preferred over a conical bottom for flow cytometry?
Round bottom tubes allow the flow cytometer's sample probe to aspirate from the very base of the tube without the restriction that a conical geometry can create, and the curved interior wall helps cell pellets resuspend evenly with minimal vortexing. The 12 × 75 mm dimension is the widely adopted standard format for most flow cytometry instrument carousels.
Is PPC autoclavable, and can these tubes be reused after sterilization?
Polypropylene Copolymer is generally autoclavable; however, because these tubes are individually packed and supplied sterile for single-use workflows, Caplugs® Evergreen does not validate or recommend reuse after autoclaving. For applications requiring reusable vessels, consult your lab's validation protocols before reprocessing.
