5.0 mL Round Bottom Test Tubes with Two-Position Snap Cap — PPC, Natural, Sterile, 12 × 75 mm
These 5.0 mL round bottom test tubes measure 12 × 75 mm and are constructed from natural Polypropylene Copolymer (PPC) — a flexible, impact-resistant resin well suited to centrifugation and repeated freeze-thaw cycles. Each tube ships individually packed and sterile, with a two-position snap cap that supports both vented and sealed operation. Carrying part number 222-2376-08I, they are a staple format for flow cytometry, cell sorting, hematology sample preparation, and serological assays where round bottom geometry is required for efficient pellet concentration.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-2376-08I |
| Volume | 5.0 mL |
| Dimensions | 12 × 75 mm |
| Bottom Style | Round Bottom |
| Cap Style | Two-Position Snap Cap (vented / sealed) |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| Sterility | Sterile |
| Pack Size | 1 / Individually Packed |
| BPA / Phthalate Free | Yes |
| Country of Origin | USA |
Applications
- Flow Cytometry & FACS: The 12 × 75 mm round bottom format is the standard geometry for flow cytometer sample probe access; natural PPC is compatible with fluorescent staining buffers and fixatives.
- Cell Sorting & Immunofluorescence: Round bottom concentrates cell pellets at the tube base after centrifugation, enabling efficient resuspension and minimal sample loss during immunostaining workflows.
- Hematology Sample Preparation: The 5.0 mL capacity and standard dimensions suit automated and manual hematology dilution and analysis protocols.
- Serological & Agglutination Assays: Round bottom geometry allows visible agglutination patterns to settle and read accurately without interference from tube corners.
- Specimen Collection & Transport: The two-position snap cap seals securely for transport and opens to a vented position for incubation, eliminating the need to swap closures between workflow steps.
- Freeze-Thaw Sample Storage: PPC flexibility and impact resistance support multiple freeze-thaw cycles without cracking, making these tubes suitable for biobanking and specimen archiving at low temperatures.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline | Excellent |
| Dilute acids | Excellent |
| Dilute bases | Excellent |
| Methanol | Compatible |
| Isopropanol | Compatible |
| Acetonitrile | Compatible |
| Aromatic solvents (e.g., toluene, xylene) | Limited — not recommended for prolonged contact |
Quality & Procurement
Made in USA at Caplugs® Evergreen ISO-certified facilities, these tubes are manufactured from virgin-grade, FDA-registered PPC resin that is BPA-free and phthalate-free. Lot-level quality controls ensure consistent tube geometry, wall thickness, and dimensional uniformity across every individually packed unit. LabSupplies.com is an authorized Caplugs® Evergreen dealer shipping from USA inventory, with volume and institutional pricing available for clinical labs, research institutions, and biotech procurement programs operating under CAP, CLIA, or ISO 15189 frameworks.
Frequently Asked Questions
Are these tubes supplied sterile, and is sterility maintained in individual packaging?
Yes — each tube is individually packed and sterile. The sealed packaging preserves sterility from the point of manufacture through point-of-use opening, making these tubes ready for cell culture, specimen collection, and other contamination-sensitive workflows without additional sterilization steps.
What does the two-position snap cap do, and can I switch between positions mid-protocol?
The two-position snap cap offers a vented configuration for gas exchange during cell incubation and a fully sealed configuration for containment during centrifugation or transport. The positions can be toggled by hand mid-protocol without tools, eliminating the need to swap or store separate closures.
Why is a round bottom preferred over a conical bottom for flow cytometry?
Round bottom tubes allow the flow cytometer's sample probe to aspirate from the very base of the tube without the restriction that a conical geometry can create, and the curved interior wall helps cell pellets resuspend evenly with minimal vortexing. The 12 × 75 mm dimension is the widely adopted standard format for most flow cytometry instrument carousels.
Is PPC autoclavable, and can these tubes be reused after sterilization?
Polypropylene Copolymer is generally autoclavable; however, because these tubes are individually packed and supplied sterile for single-use workflows, Caplugs® Evergreen does not validate or recommend reuse after autoclaving. For applications requiring reusable vessels, consult your lab's validation protocols before reprocessing.