4.0 mL Round Bottom Test Tubes — Polystyrene, Natural, Sterile, 10 × 75 mm (222-2003-010)
These 4.0 mL round bottom test tubes measure 10 × 75 mm and are manufactured from virgin-grade polystyrene (PS) for excellent optical clarity and compatibility with fluorescent dyes used in cell analysis workflows. Supplied sterile in sealed 25/Bag packaging, they are ready for use in flow cytometry, immunofluorescence, cell sorting, and routine sample preparation without any additional prep. The round bottom geometry concentrates cell pellets and precipitates at the tube base, enabling efficient resuspension and aspiration following centrifugation.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-2003-010 |
| Volume | 4.0 mL |
| Dimensions | 10 × 75 mm |
| Bottom Style | Round Bottom |
| Material | Polystyrene (PS) |
| Color | Natural |
| Cap | None (open top) |
| Sterility | Sterile |
| Pack Size | 25/Bag |
| BPA / Phthalate Free | Yes |
| Country of Origin | USA |
Applications
- Flow cytometry and FACS analysis — round bottom concentrates cell pellets for efficient resuspension after centrifugation
- Immunofluorescence staining — PS optical clarity supports visual and instrument-based inspection with standard fluorescent dyes
- Cell sorting workflows requiring consistent 10 × 75 mm tube geometry
- Hematology sample preparation and specimen handling at the 4.0 mL working volume
- Serological testing and agglutination assays where a round bottom format aids in reading reactions
- General laboratory sample collection and processing requiring sterile, lot-traceable tubes
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline | Excellent |
| Dilute acids | Good |
| Dilute bases | Good |
| Methanol | Good — short-term contact |
| Isopropanol | Good — short-term contact |
| Acetonitrile | Good — short-term contact |
| Aromatic / chlorinated solvents | Not recommended |
| Acetone | Not recommended |
| Autoclave | Not suitable — PS is not autoclavable |
Quality & Procurement
Made in USA at Caplugs® Evergreen ISO-certified facilities, these tubes are produced from virgin-grade, BPA-free, phthalate-free polystyrene to ensure consistent wall thickness, round bottom radius, and dimensional uniformity across every tube in the bag. Each bag carries a lot number to support full traceability in CAP-, CLIA-, and ISO 15189-regulated environments. LabSupplies.com is an authorized Caplugs® Evergreen dealer shipping from USA inventory, with tiered pricing available for high-volume institutional and grant-funded accounts.
Frequently Asked Questions
Are these tubes sterile out of the bag?
Yes — the tubes are supplied sterile in sealed packaging and are ready to use at the point of opening, with no additional sterilization required.
Do these tubes come with caps?
No — part number 222-2003-010 is an open-top tube with no cap included. If closure is needed for your workflow, a separately purchased cap compatible with 10 × 75 mm tubes should be selected.
Why are round bottom tubes preferred for flow cytometry?
The curved base concentrates cell pellets and precipitates into a tight mass after centrifugation, making resuspension more complete and aspiration easier than a flat bottom tube allows.
Can these tubes be used with organic solvents or autoclaved?
Polystyrene is well suited to aqueous buffers, dilute acids and bases, and short-term contact with alcohols, but it is not compatible with acetone, aromatic solvents, or chlorinated solvents. PS tubes cannot be autoclaved; use polypropylene tubes where heat sterilization is required.