40 mL Sterile 95-Pin Inoculator Assembly — Standard Single-Compartment Tray, PS, Natural (222-8600-01A)
This pre-assembled, single-use sterile inoculator pairs a 95-pin polystyrene head with a standard 40 mL single-compartment tray, delivering a consistent 10 µL per pin across all 95 positions in a single stamping operation. Supplied 5 assemblies per bag and ready for immediate use, these inoculator assemblies are purpose-built for replica plating, Steer's replicator MIC testing, and high-throughput multi-position inoculation in clinical microbiology and research settings. Single-use sterile construction eliminates the decontamination requirements associated with reusable replicators, reducing cross-contamination risk between patient isolates or compound lots.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-8600-01A |
| Assembly | 95-pin inoculator head + standard 40 mL inoculation tray |
| Pin Count | 95 pins |
| Volume Per Pin | 10 µL |
| Tray Type | Standard (single compartment) |
| Tray Volume | 40 mL |
| Material | Polystyrene (PS) |
| Color | Natural |
| Sterility | Sterile — pre-assembled |
| Pack Size | 5 assemblies/Bag |
| BPA / Phthalate / Latex | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
Applications
- Steer's replicator MIC testing: The 95-pin, 10 µL/pin format meets the standard Steer's replicator volume specification for agar dilution antibiotic susceptibility screening, enabling simultaneous inoculation of 95 isolates onto drug-supplemented agar plates in a single press.
- Antibiotic susceptibility screening: Clinical microbiology and research laboratories can inoculate 95 positions simultaneously onto selective or inhibitory agar, dramatically increasing throughput compared to manual inoculation.
- Replica plating: The pin head picks up bacterial colony suspensions from the single-compartment tray and stamps them onto selective or differential media for mutant screening, library selection, or phenotypic characterization.
- Multi-compound drug discovery screening: Each of the 95 pins can deliver a uniform 10 µL transfer, supporting simultaneous inoculation across 95 compound positions in early-stage antimicrobial or growth-inhibition studies.
- Quality control microbiology: Environmental and industrial QC labs can inoculate up to 95 test samples in one operation, improving consistency and reducing operator time.
- Mutant library and research screening: Single-use sterile format makes these assemblies well-suited for research workflows where eliminating carryover between replicates is essential.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline | Excellent |
| LB, TSB, BHI, and standard microbiology growth broths | Excellent |
| Agar / solid media contact | Excellent |
| Ethanol (<70%) | Good — short-term contact |
| Acetone / chlorinated solvents | Not recommended |
| Alcohols >70% / aromatic solvents | Not recommended |
| Autoclaving | Not recommended — PS is not autoclavable; supplied pre-sterilized for single use |
Quality & Procurement
Made in USA, Caplugs® Evergreen inoculator assemblies are manufactured from BPA-free, phthalate-free, latex-free polystyrene resin and supplied pre-assembled in sterile bags for immediate, single-use deployment — no in-house sterilization required. LabSupplies.com is an authorized dealer of Caplugs® Evergreen consumables and ships from USA inventory, typically same business day for stocking items, with tiered pricing available for high-volume clinical, industrial, and research procurement programs. Laboratories requiring related consumables may also consider the 222-8602-01A (95-pin assembly with two-split tray, 5/Bag) or the 222-8021-01T (40 mL standard inoculation tray only, 10/Box) for flexible workflow configurations.
Frequently Asked Questions
Are these inoculator assemblies sterile, and can they be resterilized for reuse?
Yes — each assembly is supplied pre-sterilized and ready for direct use. Because these are single-use items, resterilization is not recommended and not validated. Single-use sterile design eliminates the decontamination steps and cross-contamination risks associated with reusable replicator systems.
What is the difference between the standard single-compartment tray and a split tray, and when should I use each?
The standard 40 mL single-compartment tray included in this assembly holds one inoculum suspension shared by all 95 pins — ideal for workflows where every pin transfers the same bacterial suspension, such as classic Steer's replicator MIC testing with a single isolate panel. A two-split tray (available in the related 222-8602-01A assembly) divides the tray into two separate compartments so two different suspensions can be loaded simultaneously.
How many positions does one assembly inoculate, and how much inoculum does each pin transfer?
Each assembly inoculates 95 positions simultaneously, with each pin picking up and delivering approximately 10 µL of inoculum per stamping event. This volume is consistent with the standard Steer's replicator method used in agar dilution antibiotic susceptibility testing.
Is polystyrene compatible with standard microbiology media?
Yes — polystyrene performs excellently with aqueous buffers, saline, and common growth broths such as LB, TSB, and BHI, as well as with agar surface contact. It is not suitable for use with acetone, chlorinated solvents, or alcohols above 70%, and it is not autoclavable; however, since these assemblies are supplied pre-sterilized for single use, autoclaving is not required.