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Caplugs® Evergreen

50 mL Centrifuge Tubes, Screw Caps, PPC, Green — 222-3549-G8D | 20/Tray | Caplugs® Evergreen

50 mL Centrifuge Tubes, Screw Caps, PPC, Green — 222-3549-G8D | 20/Tray | Caplugs® Evergreen

Regular price $527.74
Regular price Sale price $527.74
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Quick Reference

Part Number 222-3549-G8D
Capacity 50 mL
Material Polypropylene Copolymer (PPC)
Color Natural
Base Freestanding (stands without rack)
Cap Green screw cap — pre-assembled in tray
Includes Spork No
Pack Size 20/Tray Assembled
Sterility Sterile
Origin Made in USA
Price $527.74/Tray ($26.3869/tube)

Technical Narrative

The Caplugs® Evergreen 222-3549-G8D is a 50 mL Sterile PPC Freestanding Centrifuge Tube with Green cap, 20/Tray Assembled. The 50 mL freestanding PPC tube is the sterile equivalent of the standard 50 mL polypropylene centrifuge tube — the workhorse of the cell biology and clinical lab — providing the maximum standard centrifuge tube volume in a sterile, freestanding PPC format. The 20/Tray Assembled format provides pre-assembled tubes in an open sterile rack tray for direct BSC pick-and-use.

Polypropylene Copolymer (PPC): full methanol and organic solvent compatibility, improved cold-temperature flexibility versus PS, optical clarity for pellet and gradient monitoring. BPA-free, phthalate-free, FDA-compliant,

Procurement economics: $527.74/Tray (20/Tray), $26.3869/tube. Siblings: 222-3550-G80 (50 mL PPC Green Cap 10/Bag Unassembled); 222-3529-G8D (30 mL PPC Green Cap 20/Tray Assembled). LabSupplies.com ships from USA inventory, typically same business day.

Technical Specifications

Part Number
Caplugs Item #
Capacity 50 mL
Geometry Conical bottom, freestanding base
Material Polypropylene Copolymer (PPC)
Color Natural
Cap Green screw cap
Includes Spork No
Assembly Pre-assembled in tray
Sterility Sterile
Pack Size 20/Tray Assembled
BPA / Phthalate BPA-Free, Phthalate-Free, Latex-Free
Country of Origin USA
Price $527.74/Tray ($26.3869/tube)

50 mL Sterile PPC Freestanding Centrifuge Tube Applications

The is compatible with the following sterile workflows.

Application Setting / Configuration Notes
Cell culture harvest (50 mL) Large-volume suspension culture, BSC Standard 50 mL format in sterile PPC; freestanding
PBMC isolation (Ficoll) 50 mL Ficoll density gradient, sterile PPC clarity for buffy coat interface; sterile for downstream culture
Large T-cell expansion 50 mL scale cell stimulation/culture Sterile PPC; green cap color convention
Bench centrifuge Standard 50 mL rotor, up to 3000×g Universal 50 mL rotor compatibility
Cell therapy manufacturing GMP cell wash, concentration, harvest Sterile tray-assembled for GMP batch record documentation
Sterile reagent prep 50 mL sterile media/buffer preparation PPC: broad chemical resistance; freestanding on BSC
Cold storage 4°C / —20°C / —80°C PPC: cold-temperature flexibility vs PS

Chemical Resistance — Polypropylene Copolymer (PPC)

Compatibility reference for PPC at typical laboratory conditions.

Reagent / Condition Compatibility Notes
Aqueous buffers / saline Excellent PPC inert across pH 2–12.
Cell culture media (RPMI, DMEM) Excellent Standard sterile cell culture material.
Methanol / acetonitrile (<100%) Excellent Full organic solvent compatibility — key PPC advantage over PS.
Formalin (10% NBF) Excellent Compatible for post-harvest fixation.
Ficoll / Percoll density media Excellent PPC: compatible with standard gradient media.
DMSO (<10%) Excellent Compatible with cryoprotectant concentrations.
Cold storage (—80°C) Excellent PPC: improved flexibility vs PS at cryogenic retrieval.
Dichloromethane / chloroform Not Compatible Causes cracking; do not use.

Clinical & Research Laboratory Applications

Caplugs® Evergreen 50 mL sterile PPC freestanding centrifuge tubes with green caps serve cell therapy, immunology, and clinical laboratories as the sterile large-volume centrifuge tube standard. The 50 mL freestanding PPC tube is the sterile, PPC-material equivalent of the universal 50 mL polypropylene conical — used for PBMC isolation by Ficoll density gradient centrifugation, large-volume T-cell and CAR-T expansion harvest, GMP cell washing and concentration, and any sterile 50 mL centrifugation step where PPC's material properties (cold-temperature flexibility, methanol compatibility) are specified over standard PP or PS. The 20/Tray Assembled format provides pre-assembled tubes in an open sterile tray — the preferred GMP format for cell therapy batch record documentation where assembly steps must be minimized and documented.

U.S. Manufacturing, Quality & Institutional Procurement

Caplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.

LabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can request a written quote or speak with a product specialist directly from our sales engineering team.

Frequently Asked Questions

Are the tubes sterile?

Yes. Sterile — tray sterile-packed as specified.

What is the difference between 20/Tray Assembled and 10/Bag Unassembled (222-3550-G80)?

20/Tray Assembled: tubes pre-assembled with green caps in open sterile tray — direct BSC pick-and-use, no assembly, preferred for GMP cell therapy and high-throughput BSC workflows. Higher per-tube cost. 10/Bag Unassembled: tube and cap in same bag, assembled at bench — lower per-tube cost for standard research labs.

How does the sterile 50 mL PPC tube differ from a standard PP 50 mL conical?

The 222-3549/3550 series differs in three key ways: (1) Sterile — required for direct cell culture contact and GMP cell therapy; standard PP 50 mL conicals are typically non-sterile. (2) PPC material — improved cold-temperature flexibility and full methanol compatibility vs standard PP. (3) Freestanding base — stands without a rack in the BSC. Standard 50 mL conicals require a rack or holder for upright use.

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