50 mL Centrifuge Tubes, Screw Caps, PPC, Green — 222-3549-G8D | 20/Tray | Caplugs® Evergreen

50 mL Sterile Freestanding Centrifuge Tubes with Green Screw Caps — PPC, 20/Tray Assembled (222-3549-G8D)

These 50 mL sterile polypropylene copolymer (PPC) centrifuge tubes feature a conical bottom with an integrated freestanding base, pre-assembled with green screw caps in an open sterile tray for direct pick-and-use in a biosafety cabinet. Manufactured under part number 222-3549-G8D, they deliver the maximum standard centrifuge tube volume in a sterile, chemically robust PPC format — combining full organic solvent compatibility with the optical clarity needed for pellet visualization, density gradient monitoring, and demanding cell biology workflows. Each tray ships containing 20 assembled, sterile tubes ready for immediate use.

Technical Specifications

Applications

• Cell culture harvest — large-volume suspension culture processed directly in a BSC using the sterile tray format
• PBMC isolation via Ficoll density gradient — PPC optical clarity enables clear visualization of the buffy coat interface; sterile for downstream culture
• Large-scale T-cell expansion and stimulation at the 50 mL scale
• Bench centrifuge use in standard 50 mL rotors, up to 3,000 × g
• Cell therapy manufacturing — GMP cell wash, concentration, and harvest with sterile tray-assembled format supporting batch record documentation
• Sterile reagent and media preparation (RPMI, DMEM, buffer) with freestanding stability on the BSC work surface
• Cold storage at 4 °C, −20 °C, and −80 °C — PPC retains flexibility at cryogenic temperatures where polystyrene becomes brittle

Chemical Resistance

Quality & Procurement

Made in USA, these Caplugs® Evergreen centrifuge tubes are produced from FDA-compliant, BPA-free, phthalate-free, latex-free PPC resin. The sterile tray-assembled format supports GMP batch record documentation and minimizes handling time in biosafety cabinet workflows. LabSupplies.com is an authorized dealer of Caplugs® Evergreen consumables and ships from USA inventory, typically the same business day for stocking items. Laboratories requiring related formats may also consider the 222-3550-G80 (50 mL PPC, green cap, 10/Bag unassembled) or the 222-3529-G8D (30 mL PPC, green cap, 20/Tray assembled). Tiered pricing is available for high-volume institutional and grant-funded accounts.

Frequently Asked Questions

Are these centrifuge tubes sterile, and how are they packaged?
Yes — each tube arrives pre-assembled with its green screw cap and is supplied sterile in a 20/Tray format. The open tray design allows direct pick-and-use inside a biosafety cabinet without the need to transfer tubes to a separate rack before use.

Why choose PPC over standard polypropylene or polystyrene for 50 mL centrifuge tubes?
Polypropylene copolymer (PPC) offers broader chemical resistance than polystyrene — including compatibility with methanol, acetonitrile, formalin, and DMSO — while providing improved cold-temperature flexibility compared to both PS and standard PP. PPC optical clarity also makes it easier to monitor pellets and density-gradient interfaces without removing the tube from the rotor.

What is the maximum recommended centrifugation speed for these tubes?
These tubes are designed for use in standard bench centrifuge 50 mL rotors at up to 3,000 × g. Always verify rotor and centrifuge manufacturer guidelines for maximum rated g-force with 50 mL conical tubes before use.

Are these tubes suitable for cryogenic storage at −80 °C?
Yes. PPC retains flexibility at cryogenic temperatures — a distinct advantage over polystyrene, which becomes brittle below freezing — making these tubes appropriate for cold storage at 4 °C, −20 °C, and −80 °C.