Caplugs® Evergreen
50 mL Centrifuge Tubes, Screw Caps, PPC, Green — 222-3549-G8D | 20/Tray | Caplugs® Evergreen
50 mL Centrifuge Tubes, Screw Caps, PPC, Green — 222-3549-G8D | 20/Tray | Caplugs® Evergreen
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Quick Reference
| Part Number | 222-3549-G8D |
|---|---|
| Capacity | 50 mL |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| Base | Freestanding (stands without rack) |
| Cap | Green screw cap — pre-assembled in tray |
| Includes Spork | No |
| Pack Size | 20/Tray Assembled |
| Sterility | Sterile |
| Origin | Made in USA |
| Price | $527.74/Tray ($26.3869/tube) |
Technical Narrative
The Caplugs® Evergreen 222-3549-G8D is a 50 mL Sterile PPC Freestanding Centrifuge Tube with Green cap, 20/Tray Assembled. The 50 mL freestanding PPC tube is the sterile equivalent of the standard 50 mL polypropylene centrifuge tube — the workhorse of the cell biology and clinical lab — providing the maximum standard centrifuge tube volume in a sterile, freestanding PPC format. The 20/Tray Assembled format provides pre-assembled tubes in an open sterile rack tray for direct BSC pick-and-use.
Polypropylene Copolymer (PPC): full methanol and organic solvent compatibility, improved cold-temperature flexibility versus PS, optical clarity for pellet and gradient monitoring. BPA-free, phthalate-free, FDA-compliant,
Procurement economics: $527.74/Tray (20/Tray), $26.3869/tube. Siblings: 222-3550-G80 (50 mL PPC Green Cap 10/Bag Unassembled); 222-3529-G8D (30 mL PPC Green Cap 20/Tray Assembled). LabSupplies.com ships from USA inventory, typically same business day.
Technical Specifications
| Part Number | |
|---|---|
| Caplugs Item # | |
| Capacity | 50 mL |
| Geometry | Conical bottom, freestanding base |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| Cap | Green screw cap |
| Includes Spork | No |
| Assembly | Pre-assembled in tray |
| Sterility | Sterile |
| Pack Size | 20/Tray Assembled |
| BPA / Phthalate | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
| Price | $527.74/Tray ($26.3869/tube) |
50 mL Sterile PPC Freestanding Centrifuge Tube Applications
The is compatible with the following sterile workflows.
| Application | Setting / Configuration | Notes |
|---|---|---|
| Cell culture harvest (50 mL) | Large-volume suspension culture, BSC | Standard 50 mL format in sterile PPC; freestanding |
| PBMC isolation (Ficoll) | 50 mL Ficoll density gradient, sterile | PPC clarity for buffy coat interface; sterile for downstream culture |
| Large T-cell expansion | 50 mL scale cell stimulation/culture | Sterile PPC; green cap color convention |
| Bench centrifuge | Standard 50 mL rotor, up to 3000×g | Universal 50 mL rotor compatibility |
| Cell therapy manufacturing | GMP cell wash, concentration, harvest | Sterile tray-assembled for GMP batch record documentation |
| Sterile reagent prep | 50 mL sterile media/buffer preparation | PPC: broad chemical resistance; freestanding on BSC |
| Cold storage | 4°C / —20°C / —80°C | PPC: cold-temperature flexibility vs PS |
Chemical Resistance — Polypropylene Copolymer (PPC)
Compatibility reference for PPC at typical laboratory conditions.
| Reagent / Condition | Compatibility | Notes |
|---|---|---|
| Aqueous buffers / saline | Excellent | PPC inert across pH 2–12. |
| Cell culture media (RPMI, DMEM) | Excellent | Standard sterile cell culture material. |
| Methanol / acetonitrile (<100%) | Excellent | Full organic solvent compatibility — key PPC advantage over PS. |
| Formalin (10% NBF) | Excellent | Compatible for post-harvest fixation. |
| Ficoll / Percoll density media | Excellent | PPC: compatible with standard gradient media. |
| DMSO (<10%) | Excellent | Compatible with cryoprotectant concentrations. |
| Cold storage (—80°C) | Excellent | PPC: improved flexibility vs PS at cryogenic retrieval. |
| Dichloromethane / chloroform | Not Compatible | Causes cracking; do not use. |
Clinical & Research Laboratory Applications
Caplugs® Evergreen 50 mL sterile PPC freestanding centrifuge tubes with green caps serve cell therapy, immunology, and clinical laboratories as the sterile large-volume centrifuge tube standard. The 50 mL freestanding PPC tube is the sterile, PPC-material equivalent of the universal 50 mL polypropylene conical — used for PBMC isolation by Ficoll density gradient centrifugation, large-volume T-cell and CAR-T expansion harvest, GMP cell washing and concentration, and any sterile 50 mL centrifugation step where PPC's material properties (cold-temperature flexibility, methanol compatibility) are specified over standard PP or PS. The 20/Tray Assembled format provides pre-assembled tubes in an open sterile tray — the preferred GMP format for cell therapy batch record documentation where assembly steps must be minimized and documented.
U.S. Manufacturing, Quality & Institutional Procurement
Caplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.
LabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can request a written quote or speak with a product specialist directly from our sales engineering team.
Frequently Asked Questions
Are the tubes sterile?
Yes. Sterile — tray sterile-packed as specified.
What is the difference between 20/Tray Assembled and 10/Bag Unassembled (222-3550-G80)?
20/Tray Assembled: tubes pre-assembled with green caps in open sterile tray — direct BSC pick-and-use, no assembly, preferred for GMP cell therapy and high-throughput BSC workflows. Higher per-tube cost. 10/Bag Unassembled: tube and cap in same bag, assembled at bench — lower per-tube cost for standard research labs.
How does the sterile 50 mL PPC tube differ from a standard PP 50 mL conical?
The 222-3549/3550 series differs in three key ways: (1) Sterile — required for direct cell culture contact and GMP cell therapy; standard PP 50 mL conicals are typically non-sterile. (2) PPC material — improved cold-temperature flexibility and full methanol compatibility vs standard PP. (3) Freestanding base — stands without a rack in the BSC. Standard 50 mL conicals require a rack or holder for upright use.
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