30 mL Sterile Freestanding Centrifuge Tubes with Green Screw Cap & Spork — PPC, 10/Bag Unassembled (222-3534-G80)
These 30 mL sterile polypropylene copolymer (PPC) centrifuge tubes feature a conical bottom, a natural-colored freestanding base, and a green screw cap — supplied unassembled with an integrated spork in packs of 10/Bag. The freestanding design keeps tubes upright in biosafety cabinets and on the bench without a rack, while the included spork enables direct post-centrifuge pellet or interface collection without a separate instrument. Part number 222-3534-G80 is the go-to large-volume sterile tube for cell harvest, density gradient work, and specimen processing workflows that exceed the capacity of a standard 15 mL conical.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-3534-G80 |
| Capacity | 30 mL |
| Geometry | Conical bottom, freestanding base |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural (tube) / Green (cap) |
| Cap Type | Green screw cap |
| Includes Spork | Yes — spork for post-centrifuge specimen collection |
| Assembly State | Unassembled (tube, cap, and spork loose in bag) |
| Sterility | Sterile |
| Pack Size | 10/Bag |
| BPA / Phthalate / Latex | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
Applications
- Cell culture harvest: Sterile 30 mL format supports suspension culture spin-downs in biosafety cabinet workflows; freestanding base holds tubes upright without a rack throughout the process.
- T-cell and CAR-T expansion: PPC methanol compatibility supports post-harvest intracellular cytokine staining (ICS) protocols requiring organic reagents.
- Density gradient centrifugation: PPC optical clarity allows direct visual monitoring of Ficoll/Percoll interfaces and pellets post-spin.
- Cold storage and retrieval: PPC maintains flexibility at 4 °C, –20 °C, and –80 °C, reducing cracking risk on frozen-sample retrieval.
- SPE eluate collection: Acetonitrile- and methanol-compatible PPC makes these tubes suitable for collection under sterile solid-phase extraction manifolds.
- Post-centrifuge specimen collection: The included spork allows direct pellet or sediment transfer to a slide or secondary vessel without a separate scoop, lowering per-specimen instrument count.
- Stool and environmental specimen processing: The spork enables direct specimen transfer from the freestanding tube, eliminating the need to source collection utensils separately.
- Bench centrifuge use: Compatible with standard rotor adapters up to 3,000 × g; verify that your rotor adapter accommodates the 30 mL tube outer diameter.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline / PBS | Excellent |
| Dilute acids and bases | Excellent |
| Methanol / ethanol | Good — compatible for ICS and organic wash steps |
| Acetonitrile (ACN) | Good — suitable for SPE eluate collection |
| Ficoll / Percoll gradient media | Excellent |
| Chlorinated solvents (chloroform, DCM) | Not recommended |
| Aromatic solvents (toluene, xylene) | Not recommended |
| Autoclave / dry heat sterilization | Not required — supplied sterile |
| Freeze–thaw cycling (to –80 °C) | Good — PPC cold-temperature flexibility reduces cracking |
Quality & Procurement
Made in USA, these Caplugs® Evergreen centrifuge tubes are manufactured from FDA-compliant polypropylene copolymer resin that is BPA-free, phthalate-free, and latex-free. Each unit is supplied sterile and ready for aseptic assembly at the bench or inside a biosafety cabinet. LabSupplies.com is an authorized Caplugs® Evergreen dealer shipping from USA inventory, typically same business day for stocking items, with tiered pricing available for institutional, clinical, and grant-funded laboratories.
Frequently Asked Questions
Are these centrifuge tubes supplied sterile?
Yes — every tube in the 10/Bag pack is sterile as supplied. The unassembled format lets you complete final assembly inside your biosafety cabinet or clean area immediately before use, maintaining sterility throughout your workflow.
What is the spork, and why is it included?
The spork is a combined spoon/fork laboratory utensil packaged with each tube. It allows you to collect a pellet, sediment, or interface layer directly from the centrifuge tube after spinning — eliminating the need to source a separate scoop and reducing per-specimen instrument count, particularly useful in stool and environmental specimen processing workflows.
Why choose PPC over standard polypropylene or polystyrene for centrifuge tubes?
Polypropylene copolymer (PPC) combines the organic-solvent tolerance of polypropylene — including compatibility with methanol and acetonitrile — with improved cold-temperature flexibility compared to standard polystyrene. This makes these tubes well suited for protocols that involve both organic reagents and frozen storage at –20 °C to –80 °C, while the optical clarity of PPC supports gradient and pellet monitoring.
What is the difference between this item and part number 222-3533-G8D?
Part 222-3533-G8D is the same 30 mL PPC green-cap tube with spork, but is supplied assembled in a 20/Tray format — a convenient choice when tubes need to arrive ready for immediate use. The 222-3534-G80 ships unassembled at 10/Bag, which suits labs that prefer to assemble in a biosafety cabinet or that require a smaller per-order quantity.