30 mL Sterile Freestanding Centrifuge Tubes with Green Screw Cap & Spork — PPC, 20/Tray Assembled (222-3533-G8D)
These 30 mL polypropylene copolymer (PPC) centrifuge tubes from Caplugs® Evergreen are supplied sterile, pre-assembled with a green screw cap and integrated spork, in an open rack tray of 20 for direct biosafety cabinet pick-and-use. The conical bottom with freestanding base keeps tubes upright throughout the workflow — no rack required — while the included spork eliminates the need for a separate collection instrument during post-centrifuge pellet or interface recovery. Part number 222-3533-G8D is the go-to large-volume sterile conical for cell harvest, suspension culture, and specimen processing workflows that exceed standard 15 mL capacity.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-3533-G8D |
| Capacity | 30 mL |
| Geometry | Conical bottom, freestanding base |
| Material | Polypropylene Copolymer (PPC) |
| Tube Color | Natural (clear) |
| Cap Color | Green screw cap |
| Includes Spork | Yes — integrated spork for specimen collection |
| Assembly | Pre-assembled in tray |
| Sterility | Sterile |
| Pack Size | 20/Tray Assembled |
| BPA / Phthalate / Latex | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
Applications
- Cell culture harvest: Sterile 30 mL conical format accommodates suspension culture volumes beyond standard 15 mL capacity; freestanding base holds tubes upright in the BSC without a separate rack.
- T-cell and CAR-T expansion: PPC's methanol compatibility supports post-harvest intracellular cytokine staining (ICS) and other organic solvent steps in large-scale T-cell workflows.
- Density gradient centrifugation: PPC optical clarity allows real-time monitoring of Ficoll/Percoll interfaces and pellets; the freestanding base keeps the tube stable after the spin.
- SPE eluate collection under sterile manifolds: PPC resists acetonitrile and methanol, making these tubes suitable for solid-phase extraction eluates in sterile analytical workflows.
- Cold storage: Improved cold-temperature flexibility versus standard polypropylene reduces brittleness during retrieval from –20°C or –80°C freezers.
- Post-centrifuge specimen collection: The included spork allows direct pellet or sediment transfer from the tube without sourcing a separate scoop, reducing per-specimen instrument count.
- Stool and environmental specimen processing: The spork enables direct transfer from the freestanding tube to a slide or secondary vessel, streamlining stool O&P and environmental testing workflows.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers and saline | Excellent |
| Dilute acids and bases | Excellent |
| Methanol / ethanol | Good — compatible for typical laboratory contact times |
| Acetonitrile (ACN) | Good — compatible for SPE and analytical workflows |
| Ficoll / Percoll gradient media | Excellent |
| Chlorinated solvents (e.g., chloroform) | Not recommended |
| Aromatic solvents (e.g., toluene, xylene) | Not recommended |
| Cold storage (–80°C) | Good — PPC maintains flexibility at low temperatures |
Quality & Procurement
Made in USA, these Caplugs® Evergreen centrifuge tubes are manufactured from FDA-compliant, BPA-free, phthalate-free, and latex-free polypropylene copolymer resin. Each tray is supplied pre-assembled and sterile for immediate BSC use, removing the manual assembly step from your workflow. LabSupplies.com is an authorized Caplugs® Evergreen dealer and ships from USA inventory, typically same business day for stocking items. Tiered pricing is available for high-volume institutional, hospital, and grant-funded accounts — contact us for a quote.
Frequently Asked Questions
Are these centrifuge tubes sterile as shipped?
Yes — each tube arrives pre-assembled with its green screw cap and spork and is supplied sterile, ready for direct pick-and-use from the open rack tray in a biosafety cabinet without additional preparation.
Do I need a separate rack to use these in my BSC or centrifuge?
No. The conical bottom is paired with a freestanding base that keeps the tube upright on any flat surface throughout the workflow — from BSC setup through post-spin specimen collection — without a separate rack or insert.
What is the spork, and do I have to use it?
The spork is a combined spoon/fork laboratory utensil included with each tube for post-centrifuge pellet or interface collection. It is particularly useful in stool and environmental specimen workflows where direct transfer to a slide or secondary vessel is required. Its use is optional; it does not affect centrifuge performance or tube integrity.
Is PPC compatible with the organic solvents used in density gradient and staining protocols?
Yes — polypropylene copolymer offers good compatibility with methanol and acetonitrile, supporting post-harvest ICS staining and SPE eluate collection. However, chlorinated and aromatic solvents such as chloroform or toluene are not recommended for use with PPC tubes.