25–30 mL Centrifuge Tubes, Screw Caps, PPC, Green — 222-3529-G8D | 20/Tray | Caplugs® Evergreen

Quick Reference

Technical Narrative

The Caplugs® Evergreen 222-3529-G8D is a 30 mL Sterile PPC Freestanding Centrifuge Tube with Green Cap, 20/Tray Assembled, sterile. The freestanding base allows the tube to stand upright without a rack during bench processing — eliminating rack dependency in the BSC for high-throughput cell culture workflows. The 30 mL sterile format serves large-volume cell culture harvest, suspension culture scale-up, and any sterile processing workflow requiring more than the standard 15 mL conical tube capacity. The 20/Tray Assembled format presents pre-assembled green-capped tubes in an open sterile rack tray for direct BSC pick-and-use without tube assembly steps.

Polypropylene Copolymer (PPC): full ACN/MeOH organic solvent compatibility, improved cold-temperature flexibility vs PS, optical clarity for visual pellet and gradient monitoring. BPA-free, phthalate-free, FDA-compliant,

Procurement economics: $519.55/Tray (20/Tray), $25.9775/tube. Sibling: 222-3530-G80 (30 mL PPC Green Cap 10/Bag Unassembled). LabSupplies.com ships from USA inventory, typically same business day.

Technical Specifications

30 mL Sterile PPC Freestanding Centrifuge Tube Applications

The is compatible with the following sterile large-volume workflows.

Chemical Resistance — Polypropylene Copolymer (PPC)

Compatibility reference for PPC at typical laboratory conditions.

Clinical & Research Laboratory Applications

Caplugs® Evergreen 30 mL sterile PPC freestanding centrifuge tubes with green caps serve cell therapy, cell biology, and clinical microbiology laboratories requiring sterile large-volume centrifuge tube capacity above the standard 15 mL conical. Cell therapy manufacturing uses sterile 30 mL freestanding tubes for cell harvest, washing, and concentration steps in T-cell expansion and CAR-T manufacturing workflows where the 15 mL tube is insufficient for the cell culture volume and the freestanding base allows tube handling in the BSC without a rack. PPC is specified over PS for the same reasons as other PPC sterile tubes — methanol-based intracellular staining post-harvest and cold-storage of stained cells before flow cytometry analysis. The 20/Tray Assembled format provides pre-assembled tubes in an open sterile tray for direct BSC use without assembly — the preferred format for cell therapy GMP environments where assembly steps add process steps to the batch record.

U.S. Manufacturing, Quality & Institutional Procurement

Caplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.

LabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can request a written quote or speak with a product specialist directly from our sales engineering team.

Frequently Asked Questions

Are the 30 mL freestanding centrifuge tubes sterile?

Yes. Sterile — packed in sterile tray or sterile bag as specified.

What is the difference between 20/Tray Assembled and 10/Bag Unassembled (222-3530-G80)?

20/Tray Assembled: pre-assembled green cap + tube in open sterile tray — direct BSC pick-and-use, no assembly step, preferred for cell therapy GMP and high-throughput BSC workflows. Higher per-tube cost. 10/Bag Unassembled: tube and cap in same bag, assembled at bench — lower per-tube cost for standard research labs.

Why use a sterile 30 mL tube versus the non-sterile 220-3530-080?

The sterile 222-3529/3530 series is required when the tube will directly contact cells in culture, receive a sterile specimen for downstream sterile processing, or be used in a GMP cell therapy workflow where sterility documentation is required per batch record. The non-sterile 220-3530-080 is suited to analytical sample prep, SPE eluate collection, and bench chemistry where sterility is not required.