
30 mL Sterile Freestanding Centrifuge Tubes with Green Screw Cap — PPC, 20/Tray Assembled (222-3529-G8D)
These 30 mL polypropylene copolymer (PPC) centrifuge tubes from Caplugs® Evergreen are supplied sterile and pre-assembled in an open tray of 20, with green screw caps already fitted for direct pick-and-use in a biosafety cabinet. A molded freestanding base keeps each tube upright on the bench or inside the BSC without a separate rack, streamlining high-throughput cell culture harvest, suspension culture scale-up, and other large-volume sterile workflows where the standard 15 mL conical tube falls short. The conical bottom geometry, optical clarity of PPC, and full acetonitrile/methanol compatibility make these tubes equally at home in density gradient separations and sterile solid-phase extraction protocols.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-3529-G8D |
| Capacity | 30 mL |
| Geometry | Conical bottom, freestanding base |
| Material | Polypropylene Copolymer (PPC) |
| Tube Color | Natural |
| Cap Color | Green |
| Cap Style | Screw cap, pre-assembled |
| Sterility | Sterile |
| Pack Size | 20/Tray Assembled |
| BPA / Phthalate / Latex | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
Applications
- Large-volume cell culture harvest from suspension cultures requiring capacity beyond a standard 15 mL conical tube
- T-cell expansion workflows at 25–30 mL scale, with re-sealable green screw cap for repeated sterile sampling
- Density gradient centrifugation (Ficoll/Percoll): PPC optical clarity allows visual monitoring of the buffy coat interface
- BSC rack-free processing — freestanding base keeps tubes upright throughout open-tray pick-and-use workflows
- Cold storage at 4 °C or −20 °C, where PPC's improved cold-temperature flexibility prevents cracking
- Sterile solid-phase extraction eluate collection under a SPE manifold, leveraging PPC's full compatibility with acetonitrile and methanol
- Fixation protocols using 10% neutral buffered formalin
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline (pH 2–12) | Excellent |
| Cell culture media | Excellent |
| Methanol / acetonitrile (<100%) | Excellent |
| DMSO (<10%) | Excellent |
| Formalin (10% NBF) | Excellent |
| Density gradient media (Ficoll/Percoll) | Excellent |
| Transport media (UTM/VTM) | Excellent |
| Dichloromethane / chloroform | Not recommended — causes cracking |
Quality & Procurement
Made in USA, these Caplugs® Evergreen centrifuge tubes are manufactured from FDA-compliant PPC resin that is BPA-free, phthalate-free, and latex-free. The 20/Tray Assembled format means tubes arrive pre-capped in an open sterile tray, eliminating assembly steps and reducing contamination risk in the BSC. LabSupplies.com is an authorized Caplugs® Evergreen dealer and ships from USA inventory, typically same business day for stocking items, with tiered pricing available for institutional purchasers and grant-funded laboratories. Laboratories needing a smaller, unassembled format should inquire about the companion 10/Bag configuration.
Frequently Asked Questions
Are these centrifuge tubes truly sterile, or do they require autoclaving before use?
These tubes are supplied sterile as packaged — no autoclaving is required. The pre-assembled tray format allows direct pick-and-use in a BSC straight from the tray.
Do the tubes require a rack to stand upright during centrifugation or bench processing?
No. The molded freestanding base keeps each tube upright on the benchtop and inside the BSC without any rack or stand, which is particularly valuable during open-tray workflows where a separate rack would take up biosafety cabinet space.
Is PPC suitable for organic solvents like acetonitrile and methanol?
Yes — polypropylene copolymer offers full compatibility with acetonitrile and methanol at typical laboratory concentrations, making these tubes appropriate for sterile SPE eluate collection and similar solvent-contact workflows. Chlorinated solvents such as dichloromethane and chloroform are not recommended, as they can cause cracking.
What does the green cap color indicate?
The green cap is a product-line color designation used by Caplugs® Evergreen to differentiate this 30 mL sterile PPC tube from other tube formats and capacities in the range, aiding visual identification during inventory management and bench use.