Caplugs® Evergreen
5 mL Test Tubes, Round Bottom, PPC, Natural, Beckman/BD Compatible — 222-2367-080 | 25/Bag | Caplugs® Evergreen
5 mL Test Tubes, Round Bottom, PPC, Natural, Beckman/BD Compatible — 222-2367-080 | 25/Bag | Caplugs® Evergreen
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Quick Reference
| Part Number | 222-2367-080 |
|---|---|
| Capacity | 5 mL |
| Dimensions | 12 × 75 mm |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| Cap / Closure | None — open top; use separate cap or stopper |
| Sterility | Sterile — individually or bag sterile-packed |
| Pack Size | 25/Bag |
| Origin | Made in USA |
| Price | $86.22/Bag ($3.4485/tube) |
Technical Narrative
The Caplugs® Evergreen 222-2367-080 is a 5 mL Round Bottom PPC Sterile Test Tube, 12 × 75 mm, 25/bag, sterile. The 5.0 mL 12×75 mm round-bottom tube is the universal flow cytometer sample tube format used across all major flow cytometry platforms. This is the PPC material alternative to the standard PS 12×75 mm tube (222-2012-010) — same geometry, same flow cytometer compatibility, different material. PPC provides improved cold-temperature flexibility versus PS — PPC tubes are less prone to micro-fracture when retrieved from —80°C storage or dry-ice transport, important for stained cell samples stored cold before analysis. PPC's copolymer architecture also provides robust dimensional stability through freeze-thaw cycling, supporting stain-and-freeze-read-later workflows.
Polypropylene Copolymer (PPC): optical clarity for direct visual reading of agglutination, turbidity, and cell pellet patterns. PPC's improved cold-temperature flexibility versus PS reduces brittle-fracture risk at —80°C storage temperatures. BPA-free, phthalate-free, FDA-compliant,
Procurement economics: $86.22/Bag, $3.4485/tube. Siblings: 222-2376-080 (5.0 mL PPC 12×75 mm Snap Cap Unassembled 25/Bag); 222-2012-010 (5.0 mL PS 12×75 mm Open Top 25/Bag). LabSupplies.com ships from USA inventory, typically same business day.
Technical Specifications
| Part Number | |
|---|---|
| Caplugs Item # | |
| Capacity | 5 mL |
| Dimensions | 12 × 75 mm |
| Geometry | Round bottom |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| Cap | None (open top); use compatible stopper or separate cap |
| Sterility | Sterile — individually or bag sterile-packed |
| Pack Size | 25/Bag |
| BPA / Phthalate | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
| Price | $86.22/Bag ($3.4485/tube) |
5 mL 12 × 75 mm Sterile PPC Round Bottom Tube Applications
The is compatible with the following laboratory workflows.
| Application / Instrument | Configuration | Notes |
|---|---|---|
| Flow cytometer (FACS) | Standard 12×75 mm tube holder (all major platforms) | Industry-standard 12×75 mm; PPC: improved cold-temp flexibility vs PS |
| Cell sorter | 12×75 mm sample tube position | PPC: compatible with standard cell sorting staining protocols |
| Stain and store workflow | Stain at room temp, store at —80°C before reading | PPC: reduced brittle-fracture risk vs PS at —80°C |
| Immunophenotyping | Surface marker staining, lyse/wash protocol | 5 mL for standard staining volumes |
| Cell culture (snap-cap) | Short-term culture in incubator | Two-position snap cap: vented for CO2 exchange (snap-cap SKU) |
| Centrifugation | Cell pellet, up to 600×g wash steps | Round bottom; PPC withstands centrifuge forces |
| Tube serology | Manual agglutination, blood bank | PPC clarity for visual agglutination reading |
Chemical Resistance — Polypropylene Copolymer (PPC)
Compatibility reference for PPC at typical laboratory conditions.
| Reagent / Condition | Compatibility | Notes |
|---|---|---|
| Aqueous buffers / saline | Excellent | PPC inert across pH 2–12. |
| Serum / plasma / whole blood | Excellent | Standard biological matrix; low protein-binding. |
| Formalin (10% NBF) | Excellent | Compatible for fixation steps in tube format. |
| Methanol (<100%) | Excellent | PPC: full methanol compatibility for intracellular staining. |
| Cell culture media (RPMI, DMEM) | Excellent | PPC: standard cell culture material. |
| Acetonitrile (<100%) | Excellent | PPC: full organic solvent compatibility. |
| Dichloromethane / chloroform | Not Compatible | Causes cracking; do not use. |
| Flow cytometry reagents | Excellent | PPC: low autofluorescence; cold-storage flexibility. |
Clinical & Research Laboratory Applications
Caplugs® Evergreen 5.0 mL 12×75 mm PPC sterile round-bottom test tubes serve flow cytometry, cell biology, and serology laboratories as the PPC alternative to the standard PS 12×75 mm tube for the same flow cytometer platforms. PPC is specified when the protocol includes staining-then-storing at —80°C before analysis — a workflow used in large clinical studies where all samples from a multi-week enrollment are stained individually and stored until batch analysis. PPC's improved cold-temperature flexibility reduces micro-fracture risk when tubes are retrieved from —80°C freezer racks, a concern with standard PS tubes after extended cold storage. PPC's full methanol compatibility is also advantageous for intracellular cytokine staining (ICS) protocols using methanol-based permeabilization buffers, where PS may show stress crazing with prolonged methanol contact. The 5.0 mL volume and 12×75 mm geometry are fully compatible with all standard flow cytometer tube holders.
U.S. Manufacturing, Quality & Institutional Procurement
Caplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.
LabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can request a written quote or speak with a product specialist directly from our sales engineering team.
Frequently Asked Questions
Are the test tubes sterile?
Yes. Sterile — individually or bag sterile-packed as specified.
What is the advantage of PPC over PS for flow cytometry tubes?
PPC provides improved cold-temperature flexibility — PPC tubes are less prone to micro-fracture when retrieved from —80°C freezer storage, important for stain-and-freeze-before-analysis workflows in large clinical studies. PPC also provides full methanol compatibility for intracellular staining protocols where PS may craze with prolonged methanol contact. Both PS and PPC are compatible with standard flow cytometry reagent panels and major flow cytometer platforms.
Is the 5.0 mL PPC tube compatible with the same flow cytometers as the PS tube (222-2012-010)?
Yes. Both are 5.0 mL 12×75 mm round-bottom tubes — the industry-standard flow cytometer tube format. The PPC and PS tubes are dimensionally identical and fit the same 12×75 mm tube holders on all major flow cytometer platforms (BD, Beckman Coulter, Miltenyi, Thermo, etc.). Select based on material requirements: PPC for cold-storage and methanol-permeabilization workflows, PS for standard room-temperature protocols.
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