5 mL Round Bottom Sterile PPC Test Tubes, 12 × 75 mm — Beckman/BD Compatible, 25/Bag
These 5 mL, 12 × 75 mm round-bottom test tubes are manufactured from Polypropylene Copolymer (PPC) and supplied sterile in 25/Bag packaging for flow cytometry, immunophenotyping, and cold-storage staining workflows. The 12 × 75 mm format is the universal sample tube geometry accepted by all major flow cytometer and cell sorter platforms, and the PPC material offers improved cold-temperature flexibility over standard polystyrene — reducing brittle-fracture risk when tubes are retrieved from −80 °C storage or dry-ice transport. Supplied open-top, these tubes are compatible with separately sourced caps or stoppers to suit closure preferences across different protocols.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-2367-080 |
| Capacity | 5 mL |
| Dimensions | 12 × 75 mm |
| Geometry | Round Bottom |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| Cap / Closure | None (open top) — use compatible stopper or separate cap |
| Sterility | Sterile (bag sterile-packed) |
| Pack Size | 25/Bag |
| BPA / Phthalate / Latex | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
Applications
- Flow cytometry: Fits standard 12 × 75 mm tube holders on all major flow cytometer platforms; PPC's dimensional stability supports both fresh and freeze-stored stained cell samples.
- Cell sorting: Compatible with standard cell-sorting and staining protocols requiring the universal 12 × 75 mm sample tube position.
- Stain-and-store workflows: Stain at room temperature, store at −80 °C, and read later — PPC's copolymer architecture reduces brittle-fracture risk through freeze-thaw cycling, making it preferable to PS for cold-storage steps.
- Immunophenotyping: Provides adequate volume for surface marker staining, lyse/wash, and intracellular staining protocols in the 5 mL format.
- Centrifugation: Round-bottom geometry supports cell pelleting; PPC withstands centrifuge forces at wash-step speeds up to 600 × g.
- Tube serology and agglutination: Natural PPC clarity allows direct visual reading of agglutination patterns and cell pellet morphology without tube removal.
Compatibility
The 12 × 75 mm round-bottom format is the industry-standard geometry accepted by Beckman Coulter®, BD Biosciences®, and all other major flow cytometer and cell sorter platforms that use a standard 12 × 75 mm tube holder. These tubes serve as a PPC-material alternative to the polystyrene 12 × 75 mm open-top tube, offering identical geometry and platform compatibility with the added benefit of improved cold-temperature performance for cold-chain staining and storage workflows. Confirm your instrument's tube-holder specifications before substituting from OEM or PS supply if your platform has optical-path requirements tied to specific material transmittance characteristics.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline (pH 2–12) | Excellent |
| Serum / plasma / whole blood | Excellent |
| Formalin (10% NBF) | Excellent — compatible for fixation steps |
| Methanol (<100%) | Excellent — suitable for intracellular staining protocols |
| Ethanol (<70%) | Good — short-term contact |
| Acetone / chlorinated solvents | Not recommended |
Quality & Procurement
Made in USA, these Caplugs® Evergreen tubes are produced from FDA-compliant, BPA-free, phthalate-free, and latex-free Polypropylene Copolymer resin under ISO 9001 quality management. Each bag is sterile-packed to support aseptic workflows directly from the bench. LabSupplies.com is an authorized Caplugs® Evergreen dealer shipping from USA inventory, typically same business day for stocking items, with tiered pricing available for high-volume institutional and grant-funded laboratories.
Frequently Asked Questions
Are these tubes sterile, and how are they packaged?
Yes — part number 222-2367-080 is supplied sterile in 25/Bag packaging. Each bag is sterile-packed for direct use in cell staining and other aseptic workflows without additional sterilization.
Why choose PPC over standard polystyrene 12 × 75 mm tubes for flow cytometry?
PPC's copolymer architecture provides greater cold-temperature flexibility than polystyrene, reducing the risk of micro-fractures when tubes are stored at −80 °C or transported on dry ice. This makes PPC the preferred material for stain-and-freeze-read-later workflows, while the geometry and platform compatibility remain identical to standard PS tubes.
Do these tubes come with caps?
No — they are supplied open-top. A compatible stopper or separately sourced cap should be used when closure is required. A snap-cap variant in the same PPC material and 12 × 75 mm format is available as a separate SKU for workflows that require an integrated closure.
What centrifuge speeds are appropriate for these tubes?
The round-bottom PPC construction supports typical cell-pellet wash steps. The source specifications reference compatibility with centrifuge forces up to 600 × g for wash protocols; consult your centrifuge rotor documentation and tube adapter requirements before exceeding this guideline.