Caplugs® Evergreen
50–100 µL Test Tubes, Round Bottom, PS, Natural, BD® Compatible — 222-2036-050 | 25/Bag | Caplugs® Evergreen
50–100 µL Test Tubes, Round Bottom, PS, Natural, BD® Compatible — 222-2036-050 | 25/Bag | Caplugs® Evergreen
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Quick Reference
| Part Number | 222-2036-050 |
|---|---|
| Capacity | 5.0 mL |
| Dimensions | 12 × 75 mm |
| Material | Polystyrene (PS) |
| Color | Natural |
| Cap / Closure | Two-position snap cap — clicks to vented or fully closed; ships unassembled |
| Sterility | Sterile — individually or bag sterile-packed |
| Pack Size | 25/Bag Unassembled |
| Key Use | Flow cytometry (FACS), serology, blood bank tube testing |
| Origin | Made in USA |
| Price | $103.73/Bag ($4.1490/tube) |
Technical Narrative
The Caplugs® Evergreen 222-2036-050 is a 5.0 mL Round Bottom PS Sterile Test Tube with Two-Position Snap Cap, 12 × 75 mm, 25/Bag Unassembled, sterile. The two-position snap cap clicks to two distinct positions: Position 1 (vented/partially open) — allows gas exchange during centrifugation, incubation, or culture; Position 2 (fully closed) — provides a sealed closure for storage, transport, and aerosol containment. Shipped unassembled — tube and caps are in the same bag but not pre-mated; the lab assembles each tube before use. This is the standard specification for bulk clinical serology and flow cytometry labs where assembly cost is preferred over individually pre-assembled units.
PS provides the optical clarity required for direct visual reading of agglutination reactions in tube serology — the test result is observed as a button, partial agglutinate, or dispersed cell pattern against the clear tube wall. PS also provides the low autofluorescence background important for fluorescence-based flow cytometry detection.
Procurement economics: $103.73/Bag, $4.1490/tube. Sterile 222-series round-bottom test tube siblings: 222-2036-05I (5.0 mL 12×75 mm Two-Position Snap Cap Individually Packed); 222-2012-010 (5.0 mL 12×75 mm Open Top 25/Bag); 222-2061-050 (8.0 mL 13×100 mm Two-Position Snap Cap 25/Bag Unassembled). LabSupplies.com ships from USA inventory, typically same business day.
Technical Specifications
| Part Number | |
|---|---|
| Caplugs Item # | |
| Capacity | 5.0 mL |
| Dimensions | 12 × 75 mm |
| Geometry | Round bottom |
| Material | Polystyrene (PS) |
| Color | Natural |
| Cap | Two-position snap cap — vented and fully closed positions; unassembled |
| Sterility | Sterile — individually or bag sterile-packed |
| Pack Size | 25/Bag Unassembled |
| Application | Flow cytometry, cell culture, serology requiring vented cap |
| BPA / Phthalate | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
| Price | $103.73/Bag ($4.1490/tube) |
5.0 mL 12×75 mm Sterile PS Round Bottom Tube Applications
The is the standard flow cytometry and serology tube. Compatible with the following platforms and protocols.
| Application / Instrument | Configuration | Notes |
|---|---|---|
| Flow cytometer (FACS) | Standard 12×75 mm tube holder (BD FACSCanto, LSR, Attune, CytoFLEX, etc.) | Industry-standard 12×75 mm format — fits all major flow cytometer tube holders |
| Cell sorter | 12×75 mm sample tube position | Open-top or snap-cap for sample loading; cap removed for cell sorter nozzle |
| Tube serology (blood bank) | Manual tube agglutination, centrifuge | PS clarity for visual agglutination reading |
| Immunophenotyping | Surface marker staining, lyse/wash protocol | 5 mL capacity for standard staining volumes (50–100 µL sample + 200 µL reagents) |
| Cell culture / stimulation | Short-term culture in incubator (snap-cap) | Two-position snap cap: vented for CO2 exchange, closed for transport |
| Intracellular staining | Fixation/permeabilization protocol | PS: compatible with methanol, saponin-based protocols |
| Centrifugation | Cell pellet, up to 600×g wash steps | Round bottom: uniform cell pellet; sterile for downstream culture |
Chemical Resistance — Polystyrene (PS)
Compatibility reference for PS at typical laboratory conditions.
| Reagent / Condition | Compatibility | Notes |
|---|---|---|
| Aqueous buffers / saline | Excellent | PS compatible across pH 4–10 for standard biological assays. |
| Serum / plasma / whole blood | Excellent | Standard clinical and blood bank specimen matrix. |
| Formalin (10% NBF) | Excellent | PS compatible with standard fixative for flow cytometry fixation. |
| Methanol (<100%) | Limited | Short-term compatible for intracellular staining fixation steps. |
| Saponin / detergent buffers | Good | PS compatible with saponin-based permeabilization buffers. |
| Acetone / chloroform | Not Compatible | Dissolves PS; do not use. |
| DMSO (<1%) | Good | Compatible at concentrations used in cell culture and cryopreservation. |
| Flow cytometry reagents (FITC, PE, APC, etc.) | Excellent | PS: low autofluorescence in visible wavelength range for cytometry. |
Clinical & Research Laboratory Applications
Caplugs® Evergreen 5.0 mL 12×75 mm sterile PS round-bottom tubes with two-position snap caps (unassembled) serve flow cytometry, cell culture, and serology laboratories requiring the standard 12×75 mm format with a re-sealable vented closure. The two-position snap cap is specified for cell culture and stimulation protocols in the tube format — Position 1 (vented) maintains a partially open cap during incubation in a CO2 incubator or rocker, allowing gas exchange while preventing aerosol contamination; Position 2 (fully closed) re-seals the tube for centrifugation, transport, and storage. The unassembled format is the cost-effective bulk specification for high-volume labs that prefer to assemble tubes at the bench.
U.S. Manufacturing, Quality & Institutional Procurement
Caplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.
LabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can request a written quote or speak with a product specialist directly from our sales engineering team.
Frequently Asked Questions
Are the test tubes sterile?
Yes. Sterile — individually or bag sterile-packed as specified. Maintain sterility until the bag or individual wrapper is opened.
What are the two positions of the two-position snap cap?
Position 1 (partially open/vented): the cap is clicked to the first position, leaving a small gap that allows gas exchange — used during incubation in CO2 incubators, aerobic culture, and centrifugation where pressure equalization is needed. Position 2 (fully closed): the cap clicks to a fully sealed position for storage, transport, and aerosol containment.
What does 'Unassembled' mean for the snap-cap tube?
Unassembled means the tube body and the snap cap are included in the same bag but are not pre-mated. The lab assembles each tube by pressing the cap onto the tube before use. This reduces per-tube cost vs individually pre-assembled units and is the standard specification for high-volume clinical and research labs.
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