Caplugs® Evergreen
50–100 µL Test Tubes, Round Bottom, PS, Natural, Beckman/BD Compatible — 222-2012-010 | 25/Bag | Caplugs® Evergreen
50–100 µL Test Tubes, Round Bottom, PS, Natural, Beckman/BD Compatible — 222-2012-010 | 25/Bag | Caplugs® Evergreen
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Quick Reference
| Part Number | 222-2012-010 |
|---|---|
| Capacity | 5.0 mL |
| Dimensions | 12 × 75 mm |
| Material | Polystyrene (PS) |
| Color | Natural |
| Cap / Closure | None — open-top tube; use separate stopper or cap |
| Sterility | Sterile — individually or bag sterile-packed |
| Pack Size | 25/Bag |
| Key Use | Flow cytometry (FACS), serology, blood bank tube testing |
| Origin | Made in USA |
| Price | $89.84/Bag ($3.5935/tube) |
Technical Narrative
The Caplugs® Evergreen 222-2012-010 is a 5.0 mL Round Bottom PS Sterile Test Tube, 12 × 75 mm, open top, 25/Bag, sterile. The 5.0 mL 12×75 mm round-bottom PS tube is the industry-standard flow cytometry tube — the universal format for FACS/cell sorting sample preparation, immunophenotyping, and cell biology assays. This is the same tube geometry used in virtually every commercial flow cytometer sample holder worldwide. Open-top format allows pipette access from above for sample loading and reagent dispensing before capping or reading.
PS provides the optical clarity required for direct visual reading of agglutination reactions in tube serology — the test result is observed as a button, partial agglutinate, or dispersed cell pattern against the clear tube wall. PS also provides the low autofluorescence background important for fluorescence-based flow cytometry detection.
Procurement economics: $89.84/Bag, $3.5935/tube. Sterile 222-series round-bottom test tube siblings: 222-2036-050 (5.0 mL 12×75 mm Two-Position Snap Cap 25/Bag Unassembled); 222-2036-05I (5.0 mL 12×75 mm Two-Position Snap Cap Individually Packed); 222-2003-010 (4.0 mL 10×75 mm Open Top 25/Bag). LabSupplies.com ships from USA inventory, typically same business day.
Technical Specifications
| Part Number | |
|---|---|
| Caplugs Item # | |
| Capacity | 5.0 mL |
| Dimensions | 12 × 75 mm |
| Geometry | Round bottom |
| Material | Polystyrene (PS) |
| Color | Natural |
| Cap | None (open top); use compatible stopper or closure |
| Sterility | Sterile — individually or bag sterile-packed |
| Pack Size | 25/Bag |
| Application | Flow cytometry (FACS), cell sorting, immunophenotyping; standard 12×75 mm format |
| BPA / Phthalate | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
| Price | $89.84/Bag ($3.5935/tube) |
5.0 mL 12×75 mm Sterile PS Round Bottom Tube Applications
The is the standard flow cytometry and serology tube. Compatible with the following platforms and protocols.
| Application / Instrument | Configuration | Notes |
|---|---|---|
| Flow cytometer (FACS) | Standard 12×75 mm tube holder (BD FACSCanto, LSR, Attune, CytoFLEX, etc.) | Industry-standard 12×75 mm format — fits all major flow cytometer tube holders |
| Cell sorter | 12×75 mm sample tube position | Open-top or snap-cap for sample loading; cap removed for cell sorter nozzle |
| Tube serology (blood bank) | Manual tube agglutination, centrifuge | PS clarity for visual agglutination reading |
| Immunophenotyping | Surface marker staining, lyse/wash protocol | 5 mL capacity for standard staining volumes (50–100 µL sample + 200 µL reagents) |
| Cell culture / stimulation | Short-term culture in incubator (snap-cap) | Two-position snap cap: vented for CO2 exchange, closed for transport |
| Intracellular staining | Fixation/permeabilization protocol | PS: compatible with methanol, saponin-based protocols |
| Centrifugation | Cell pellet, up to 600×g wash steps | Round bottom: uniform cell pellet; sterile for downstream culture |
Chemical Resistance — Polystyrene (PS)
Compatibility reference for PS at typical laboratory conditions.
| Reagent / Condition | Compatibility | Notes |
|---|---|---|
| Aqueous buffers / saline | Excellent | PS compatible across pH 4–10 for standard biological assays. |
| Serum / plasma / whole blood | Excellent | Standard clinical and blood bank specimen matrix. |
| Formalin (10% NBF) | Excellent | PS compatible with standard fixative for flow cytometry fixation. |
| Methanol (<100%) | Limited | Short-term compatible for intracellular staining fixation steps. |
| Saponin / detergent buffers | Good | PS compatible with saponin-based permeabilization buffers. |
| Acetone / chloroform | Not Compatible | Dissolves PS; do not use. |
| DMSO (<1%) | Good | Compatible at concentrations used in cell culture and cryopreservation. |
| Flow cytometry reagents (FITC, PE, APC, etc.) | Excellent | PS: low autofluorescence in visible wavelength range for cytometry. |
Clinical & Research Laboratory Applications
Caplugs® Evergreen 5.0 mL 12×75 mm sterile PS round-bottom test tubes are the universal format for flow cytometry and immunophenotyping in clinical immunology, hematology, oncology, and research settings. The 12×75 mm round-bottom tube is the standard sample tube for virtually all commercial flow cytometers — BD FACSCanto, LSRFortessa, Attune NxT, Beckman Coulter CytoFLEX, Miltenyi MACSQuant, and others all use the 12×75 mm tube holder as the primary sample input format. Clinical immunophenotyping laboratories performing CD4/CD8 T-cell counting, lymphocyte subset analysis for AIDS and lymphoma, and leukemia/lymphoma immunophenotyping by flow cytometry standardize on the sterile 5.0 mL PS round-bottom tube for staining and data acquisition. The open-top format allows pipette access from above for antibody cocktail addition, lysis buffer dispensing, and wash steps before analysis.
U.S. Manufacturing, Quality & Institutional Procurement
Caplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.
LabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can request a written quote or speak with a product specialist directly from our sales engineering team.
Frequently Asked Questions
Are the test tubes sterile?
Yes. Sterile — individually or bag sterile-packed as specified. Maintain sterility until the bag or individual wrapper is opened.
Is the 5.0 mL 12 × 75 mm tube compatible with my flow cytometer?
Yes. The 12×75 mm round-bottom tube is the industry-standard flow cytometer sample tube format used by BD (FACSCanto, LSRFortessa, Aria), Beckman Coulter (CytoFLEX, Gallios), Miltenyi (MACSQuant), Thermo (Attune), and virtually all other major flow cytometer platforms that accept standard tube input. Verify your specific instrument's tube holder specification before switching suppliers.
What is the difference between sterile (222-series) and non-sterile (214-series) round-bottom PS tubes?
The 222-series tubes are sterile-packed — required for applications involving direct patient specimen contact, cell culture, or any downstream sterile processing step. The 214-series tubes are non-sterile and suited to general diagnostic serology, mixing, and sample prep where sterility is not required. The tube material (PS) and geometry are equivalent across series.
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