1.5 mL Microcentrifuge Tubes, PP, Natural — 208-3389-060 | 200/Bag | Caplugs® Evergreen

Universal Fine-Filter SPE Columns, 15–45 µm PP — Multi-Format Gravity, Vacuum & Centrifuge Use (208-3389-060)

These Caplugs® Evergreen universal polypropylene SPE columns feature an integrated 15–45 micron fine-filter disk that delivers the highest filtrate clarity in the PP column line, making them the preferred choice for LC-MS/MS injection preparation, GC-MS confirmatory cleanup, and any protocol where residual particulates cause analytical column fouling or baseline noise. The universal body geometry adapts to 1.5 mL, 2 mL, and 5 mL collection tube positions and is compatible with both gravity-flow and vacuum manifold workflows without requiring a length-matched manifold holder. Supplied non-sterile in 200/Bag packaging, part number 208-3389-060 is a practical high-throughput option for labs running diverse SPE protocols across multiple platform types.

Technical Specifications

Applications

• LC-MS/MS injection preparation requiring maximum particulate removal before analytical column entry
• GC-MS confirmatory cleanup where filtrate clarity directly affects detection baselines
• Dissolution testing filtration in pharmaceutical QC workflows
• Environmental and biological matrix SPE cleanup across varied sample types
• Method scouting and development where a universal geometry eliminates format-specific column lock-in
• Protein precipitation clarification prior to instrumental analysis

Compatibility

The universal column body is engineered to seat in standard 1.5 mL microcentrifuge tube positions for bench centrifuge runs (up to approximately 3000×g), 2 mL collection tube positions for gravity-flow or centrifuge workflows, and 5 mL collection tube positions for larger sample-load protocols (verify column outer diameter fit with your specific 5 mL tube format before use). For vacuum-driven workflows, the universal geometry adapts to multi-port SPE manifolds with universal tube holders, accommodating various manifold port sizes without a dedicated length-matched holder. Gravity-flow bench stands are also supported, where the fine 15–45 µm filter pore size is particularly well suited to high-clarity filtrate requirements. Labs building out a complete Universal PP column set can pair these fine-filter columns with the 208-3387-060 (Coarse Filter, 90–135 µm) and 208-3388-060 (Medium Filter, 45–90 µm) for full-range pore-size coverage across the same universal platform.

Chemical Resistance

Quality & Procurement

Made in USA, these Caplugs® Evergreen SPE columns are manufactured under ISO 9001 quality management from FDA-compliant, BPA-free, phthalate-free polypropylene resin, with the fine-filter disk molded integrally into each column body for consistent pore geometry across the full lot. LabSupplies.com is an authorized dealer of Caplugs® Evergreen consumables and ships from USA inventory, typically same business day for stocking items, with volume pricing available at ten or more bags for institutional, clinical, and grant-funded laboratories.

Frequently Asked Questions

What makes the fine-filter grade different from the coarse or medium options?
The 15–45 µm pore size of these columns provides the highest filtrate clarity in the Universal PP column series — ideal for sensitive downstream techniques such as LC-MS/MS and GC-MS where even small particulates can foul analytical columns or elevate baseline noise. The coarse (90–135 µm) and medium (45–90 µm) grades are better suited to higher-viscosity matrices or workflows where throughput is prioritized over maximum clarity.

Are these columns compatible with vacuum manifolds as well as centrifuges?
Yes. The universal body geometry is designed for gravity-flow, vacuum manifold, and centrifuge workflows without requiring a length-matched manifold holder. For vacuum manifolds, confirm that your manifold uses a universal tube holder configuration to ensure a proper seat.

Are these SPE columns sterile?
No — they are supplied non-sterile in 200/Bag packaging. For applications requiring sterile filtration, additional downstream processing steps should be incorporated per your laboratory's validated method.

Can polypropylene columns be used with acetonitrile and methanol mobile phases?
Yes. Polypropylene offers excellent compatibility with acetonitrile, methanol, aqueous buffers, and dilute acids and bases — the solvents most commonly used in SPE conditioning, washing, and elution steps. Chlorinated and aromatic solvents should be avoided, as PP has limited resistance to those chemical classes.