12-Well Flat-Bottom Microplates with Lids, 6.76 mL/Well — Sterile PS, Natural, 100/Individually Packed
These sterile 12-well polystyrene microplates feature a flat-bottom geometry and an included lid, delivering 6.76 mL of working volume per well for large-volume cell culture, immunoassay, and absorbance-based workflows. Supplied as part number 222-8046-01F, each plate arrives individually packed to preserve sterility at the point of use. The flat optical surface ensures uniform absorbance path length across all wells, making these plates a reliable choice for microplate reader applications from ELISA to fluorescence detection.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-8046-01F |
| Well Format | 12-Well |
| Well Volume | 6.76 mL |
| Bottom Style | Flat Bottom |
| Lid Included | Yes |
| Material | Polystyrene (PS) |
| Color | Natural (Clear) |
| Sterility | Sterile |
| Pack Size | 100 / Individually Packed |
| BPA / Phthalate Free | Yes |
| Country of Origin | USA |
Applications
- ELISA and colorimetric immunoassay: flat-bottom geometry provides a uniform optical path length for accurate absorbance measurements in microplate readers
- Fluorescence-based assays requiring a flat optical surface and high PS optical clarity
- Large-volume cell culture and cell-based assays where the 6.76 mL per-well capacity accommodates generous media volumes
- Immunofluorescence staining workflows that benefit from the included lid for contamination prevention during extended incubation
- Microplate washer and automated liquid handling workflows on standard SBS footprint instruments
- Point-of-use aseptic technique in CAP-, CLIA-, and ISO 15189-regulated laboratories, supported by individual sterile packaging and lot-level traceability
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline | Excellent |
| Dilute acids | Excellent |
| Dilute bases | Excellent |
| Methanol | Good — short-term contact |
| Acetonitrile | Limited — use with caution |
| Ethyl acetate | Not recommended |
| Aromatic / chlorinated solvents | Not recommended |
Quality & Procurement
Made in USA under ISO-certified manufacturing, these Caplugs® Evergreen microplates are produced from BPA-free, phthalate-free polystyrene resin with lot-level quality controls to support traceability in regulated clinical and research environments. Individual sterile packaging eliminates the need for in-house sterilization and simplifies compliance documentation. LabSupplies.com is an authorized dealer of Caplugs® Evergreen laboratory consumables, shipping from USA inventory with tiered pricing available for high-volume institutional and grant-funded procurement.
Frequently Asked Questions
Are these microplates sterile, and does the lid ship sterile as well?
Yes — each plate and its accompanying lid are sterile and individually packed, so both arrive ready for aseptic technique directly from the packaging without additional sterilization steps.
Do these plates fit standard multichannel pipettes and automated liquid handlers?
Yes. The plates conform to the standard SBS footprint and 12-well spacing, making them compatible with standard single- and multichannel pipettes as well as automated liquid handling systems and microplate washers designed for that format.
Can polystyrene microplates be used with organic solvents such as acetone or ethanol?
Polystyrene performs well with aqueous buffers, dilute acids and bases, and methanol in short-term contact, but it is not recommended for use with acetone, ethyl acetate, aromatic solvents, or chlorinated solvents, which can craze or dissolve the material. Review your specific reagent compatibility before use.
What is the maximum working volume per well?
Each well holds 6.76 mL, making these plates well suited for large-volume cell culture, media changes, and assay workflows that exceed the capacity of standard 24- or 96-well formats.