VEE GEE 45000-OS025 is an oil-based refractometer calibration standard with a certified value of 60.0% Brix / 1.4400 nD (at 25.0°C, ±0.0002 nD), supplied as a 7.4 mL (0.25 oz) bottle with a Letter of NIST Traceability Certification. Intended for verifying and calibrating VEE GEE handheld and Abbe refractometers at the high end of typical Brix measuring ranges, the oil-based formulation provides a stable, non-evaporating standard that holds its value longer than aqueous standards under ambient lab conditions.
Features & Benefits
- NIST Traceable Certification: Each bottle is accompanied by a Letter of NIST Traceability Certification — the documentation required for ISO, GLP, and GMP compliance audits that mandate refractometer calibration verification with traceable standards.
- Oil-Based Formula: Unlike aqueous (water-based) Brix standards, the oil-based formulation does not evaporate on the prism during reading, providing a more stable working surface for single-operator and multi-operator calibration checks.
- High-Range Verification Point: At 60.0% Brix, the 45000-OS025 verifies refractometer accuracy at the upper range of common Brix scales — ideal for instruments used to measure concentrated juices, syrups, honey, and high-sugar solutions where low-end aqueous calibration alone is insufficient.
- Dual Reference Values: Certified at both 20°C (60% Brix, 1.4420 nD) and 25°C (60% Brix, 1.4400 nD), providing reference values applicable across standard laboratory temperature ranges without separate temperature correction.
- Made in USA: Manufactured domestically — important for labs with domestic sourcing requirements or supply chain documentation needs.
- 1-Year Warranty: Covered by VEE GEE Scientific's standard accessory warranty.
Specifications
| Property | Value |
|---|---|
| SKU | 45000-OS025 |
| Standard Type | Oil-based |
| Certified Brix Value (20°C) | 60.0% |
| Certified nD Value (20°C) | 1.4420 nD |
| Certified nD Value (25°C) | 1.4400 nD |
| Tolerance | ±0.0002 nD |
| Volume | 7.4 mL (0.25 oz) |
| Certification | Letter of NIST Traceability |
| Color | Clear |
| Quantity | 1 Each |
| Dimensions (L × W × H) | 1.0" × 1.0" × 2.25" (2.5 × 2.5 × 5.7 cm) |
| Warranty | 1 Year |
| Country of Origin | USA |
| Brand | VEE GEE Scientific |
Laboratory Applications
The 45000-OS025 is used to verify refractometer accuracy and document calibration in any regulated or quality-controlled environment that requires traceable calibration records. Food and beverage QC labs use it to verify instruments measuring concentrated syrups, honey, jams, and high-Brix fruit concentrates before production runs. Pharmaceutical and cosmetic labs use it to meet GMP calibration documentation requirements. Industrial labs use it to verify instruments monitoring high-concentration process fluids. The oil-based standard pairs with the water-based zero-point calibration that refractometers perform daily — together they provide a two-point calibration verification spanning the instrument's full range. Compatible with VEE GEE X-Series handheld refractometers including the 43001, 43002 (BTX-1), 43003, 43005, 43009, and others. Note: not compatible with the CDX digital refractometer series.
Frequently Asked Questions
What is the difference between an oil-based and a water-based refractometer standard?
Water-based standards (aqueous) are used for zero-point calibration — placing distilled water on the prism should read 0% Brix. Oil-based standards like the 45000-OS025 verify accuracy at a defined non-zero point (here, 60% Brix) without evaporating off the prism during measurement. Because oil does not evaporate under ambient conditions, it provides a stable surface for repeatable readings, which is particularly valuable for multi-operator calibration checks and documented GMP/GLP verification records.
Does the 45000-OS025 come with a NIST certificate?
Yes. Each bottle of 45000-OS025 is supplied with a Letter of NIST Traceability Certification documenting the certified Brix and refractive index values. This letter satisfies the calibration documentation requirement for ISO 17025, GLP, GMP, and HACCP audits that mandate traceable calibration records for optical measurement instruments.
Which VEE GEE refractometers is the 45000-OS025 compatible with?
The 45000-OS025 is compatible with most VEE GEE handheld X-Series refractometers and Abbe refractometers. It is specifically not compatible with the CDX digital refractometer series. For any refractometer with a Brix scale reaching 60%, the 45000-OS025 provides a useful upper-range verification point.
How do I use the 45000-OS025 to calibrate my refractometer?
First zero-calibrate your refractometer with distilled water. Then place 1–2 drops of the 45000-OS025 oil standard on the prism and close the daylight plate. Read the Brix value through the eyepiece — it should read 60.0% (±0.2%, which is the instrument's rated accuracy). If the reading deviates beyond the instrument's stated accuracy, recalibrate per the instrument manual. Clean the prism thoroughly with the microfiber cloth after use, as oil residue will affect subsequent measurements.
Ordering
Authorized dealer, ships from USA inventory. Serving academic, government, and research laboratories nationwide.