5.0 mL Round Bottom Test Tubes with Two-Position Snap Caps — PPC, Natural, Sterile, 25/Bag
These 5.0 mL round bottom test tubes measure 12 × 75 mm and are constructed from virgin-grade Polypropylene Copolymer (PPC) in a natural (translucent) finish, supplied sterile and unassembled in 25/Bag packaging. The round bottom geometry concentrates cell pellets and precipitates at the tube base for efficient resuspension and aspiration after centrifugation. Each tube is paired with a two-position snap cap that toggles between a vented configuration for gas exchange during incubation and a fully sealed position for containment during transport or storage.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-2376-080 |
| Volume | 5.0 mL |
| Dimensions | 12 × 75 mm |
| Bottom Style | Round Bottom |
| Cap Type | Two-Position Snap Cap (Vented / Sealed) |
| Assembly | Unassembled (tubes and caps packaged separately) |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| Sterility | Sterile |
| Pack Size | 25/Bag |
| BPA / Phthalate Free | Yes |
| Country of Origin | USA |
Applications
- Flow cytometry and FACS analysis — the standard 12 × 75 mm round bottom format is compatible with most cytometer sample introduction systems
- Immunofluorescence staining protocols requiring multiple wash and resuspension steps
- Cell sorting and pelleting — round bottom concentrates cells at a single point for complete, low-loss resuspension
- Hematology sample preparation at the 5.0 mL working volume
- Serological testing and agglutination assays where round bottom format is required for reading precipitate patterns
- Specimen collection and general laboratory sample handling where sterile, sealed packaging is required at point of receipt
- Freeze-thaw cycling applications — PPC flexibility and impact resistance reduce cracking under thermal stress
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline | Excellent |
| Dilute acids | Excellent |
| Dilute bases | Excellent |
| Methanol | Compatible |
| Isopropanol | Compatible |
| Acetonitrile | Compatible |
| Aromatic solvents (benzene, toluene, xylene) | Use with caution — limited resistance |
| Chlorinated solvents (chloroform, DCM) | Not recommended |
Quality & Procurement
Made in USA at Caplugs® Evergreen ISO-certified manufacturing facilities using domestically sourced, FDA-registered virgin-grade PPC resin, these tubes are free of BPA and phthalates. Lot-level quality controls ensure consistent tube geometry, wall thickness, and dimensional uniformity across every bag, supporting compliance in CAP-, CLIA-, and ISO 15189-accredited laboratories. LabSupplies.com is an authorized Caplugs® Evergreen dealer, shipping from USA inventory with tiered volume pricing available for institutional, hospital, and grant-funded procurement programs.
Frequently Asked Questions
Are these tubes supplied already assembled with the snap caps attached?
No — part number 222-2376-080 is supplied unassembled, meaning tubes and snap caps are packaged separately within the bag. This preserves sterility and allows users to assemble each tube immediately before use.
What does the two-position snap cap do, and when would I use each position?
The snap cap has two distinct locked positions: a vented position that allows gas exchange — useful during cell incubation steps — and a fully sealed position that provides containment for centrifugation, transport, or storage. Switching between positions requires a simple push of the cap.
Are these tubes compatible with standard flow cytometry instruments?
Yes. The 12 × 75 mm round bottom format is the widely accepted standard for flow cytometry sample introduction and is compatible with most cytometer sample probes and carousel-style autosampler systems. Confirm your instrument's tube diameter specification before ordering in high volume.
Can these tubes withstand freeze-thaw cycling and centrifugation?
Polypropylene Copolymer (PPC) offers greater flexibility and impact resistance than standard homopolymer polypropylene, making these tubes well suited for centrifugation and repeated freeze-thaw cycles. Always confirm your centrifuge manufacturer's tube-compatibility ratings before use at high g-forces.