0.4 mL CryoSure® Round-Bottom Cryogenic Vials — PPC, Natural, 1000/Bag (220-3925-080)
These 0.4 mL CryoSure® round-bottom cryogenic vials from Caplugs® Evergreen are constructed from polypropylene copolymer (PPC), a material engineered to remain flexible and crack-resistant across the full cryogenic temperature range — from –80°C ultra-low temperature (ULT) freezers through liquid nitrogen (LN₂) storage at –196°C. Supplied non-sterile in 1000/Bag bulk packaging, part number 220-3925-080 is well suited for biobanking, cell line preservation, and clinical biospecimen archiving where consistent microvolume recovery is essential. The round bottom concentrates sample at the lowest point of the vial, enabling thorough recovery of small aliquots after thaw.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 220-3925-080 |
| Volume | 0.4 mL |
| Vial Type | CryoSure® Cryogenic Round Vial |
| Bottom Style | Round Bottom |
| Cap Style | Snap Cap |
| Cryogenic Rating | LN₂ / Vapor-Phase N₂ / –80°C ULT |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| Sterility | Non-Sterile |
| Pack Size | 1000/Bag |
| BPA / Phthalate Free | Yes |
| Country of Origin | USA |
Applications
- Biobanking and long-term biological sample archiving in LN₂ or vapor-phase nitrogen dewars
- Cell line and primary cell cryopreservation at –80°C ULT or below
- DNA and RNA aliquot storage where microvolume recovery and sample integrity are critical
- Serum and plasma aliquot cryopreservation for clinical and translational research workflows
- Tissue sample archiving for CAP-, CLIA-, and ISO 15189-regulated clinical laboratories
- Dry ice shipment of cryogenic biospecimens requiring vials rated for extreme cold
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline | Excellent |
| Dilute acids | Excellent |
| Dilute bases | Excellent |
| Methanol | Compatible |
| Isopropanol (IPA) | Compatible |
| Acetonitrile | Compatible |
| Aromatic / chlorinated solvents | Limited — verify before use |
Quality & Procurement
Made in USA, these CryoSure® vials are produced at Caplugs® Evergreen ISO-certified facilities using virgin-grade PPC resin that is BPA-free and phthalate-free. Lot-level quality controls ensure consistent vial geometry, wall thickness, and snap-cap seal integrity across every vial in the bag — providing the traceability documentation required by CAP, CLIA, and ISO 15189 accredited laboratories. LabSupplies.com is an authorized Caplugs® Evergreen dealer shipping from USA inventory, with tiered pricing available for high-volume institutional and grant-funded accounts.
Frequently Asked Questions
Are these vials sterile?
No — the 0.4 mL CryoSure® vials are supplied non-sterile. For applications requiring sterile consumables, consider a DNase/RNase-decontamination step or inquire about sterile configurations before ordering.
Why use PPC instead of standard polypropylene for cryogenic storage?
Standard PP homopolymer becomes brittle below approximately –40°C and can crack under thermal stress during freeze-thaw cycling. Polypropylene copolymer (PPC) retains flexibility and impact resistance down to –196°C, making it the preferred material for LN₂ and vapor-phase nitrogen applications.
What is the advantage of a round-bottom design at 0.4 mL?
At microvolumes, a round bottom concentrates the sample at the lowest point of the vial, minimizing dead volume and allowing more complete recovery after thawing — particularly important when working with precious or limited biospecimens.
Does each bag include lot traceability information?
Yes. Every 1000/Bag shipment includes lot number and manufacturing date documentation to support the chain-of-custody and traceability requirements of CAP-, CLIA-, and ISO 15189-accredited laboratories.