Caplugs® Evergreen
2–4 mL Deep Well Microplates, Chamfered, PPC, Natural — 290-8560-08C | 5/Bag | Caplugs® Evergreen
2–4 mL Deep Well Microplates, Chamfered, PPC, Natural — 290-8560-08C | 5/Bag | Caplugs® Evergreen
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48-Well Deep Well Microplates, Chamfered Bottom, 5.0 mL Well Volume, Polypropylene Copolymer (PPC), Natural — 290-8560-08C | 5/Bag (20/Case)
| Quick Reference — 290-8560-08C | |
|---|---|
| Well Format | 48-well SBS/ANSI |
| Bottom | Chamfered |
| Well Volume | 5.0 mL per well |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| DMSO Compat. | ✓ All — superior to PP |
| Cryo Storage | ✓ -80°C ultra-low temp |
| Autoclave | ✓ Compatible |
| Pack Size | 5/Bag (20/Case) |
| Price | $90.19 / Bag ($4.5095 / plate) |
Technical Narrative
The Caplugs® Evergreen is a 48-well deep-well Polypropylene Copolymer (PPC) microplate with 5.0 mL per well — ~14× vs 24-well the capacity of standard 24-well plates — in the SBS/ANSI standard footprint compatible with all standard plate liquid handlers, centrifuge adapters, and plate readers. The deep-well format eliminates the volume constraint of standard plates for large-scale cell culture, organoid culture, bioprocess development. The chamfered bottom guides pipette tips and robotic needle arrays toward the well center during automated dispensing and aspiration — reducing tip-crash events and improving recovery accuracy in high-speed automated sample prep. Polypropylene Copolymer (PPC) construction provides DMSO + -80°C cryo + autoclave.
PPC provides the highest chemical and temperature resistance of the three deep-well material options: superior DMSO resistance vs PP, -80°C ultra-low temperature stability for cryo archive deep-well plates (where standard PP can become brittle), and autoclave compatibility at 121°C. PPC's copolymer toughness maintains deep-well geometry through repeated -80°C freeze-thaw cycling — critical for deep-well compound archive plates that cycle between cryo storage and ambient-temperature dispensing.
At $90.19 per 5/Bag — $4.5095 per plate — the delivers 5.0 mL deep-well PPC capacity at per-plate economics for sample prep programs. Also available: 290-8556-08C (96-well PPC 2.0 mL chamfered deep well).
Technical Specifications — Caplugs® Evergreen
| Specification | Value |
|---|---|
| Part Number | |
| Caplugs Item # | 290856008CE1 |
| Well Format | 48-well SBS/ANSI |
| Bottom | Chamfered |
| Well Volume | 5.0 mL per well |
| Material | Polypropylene Copolymer (PPC) |
| Color | Natural |
| DMSO Compat. | ✓ All concentrations — superior to PP (PPC) |
| Cryo Storage | ✓ -80°C suitable — key advantage |
| Autoclave | ✓ Compatible (121°C) |
| Pack Size | 5/Bag (20/Case) |
| BPA/Phthalate | BPA-free; no phthalate plasticizers |
| Country of Origin | Made in U.S.A. (California) |
48-Well 5.0 mL Deep Well Plate Compatibility — PPC
The 5.0 mL 48-well deep well format provides ~14× vs 24-well the volume of standard 24-well plates. Key applications for the PPC format:
| Application | Assay / Use | Notes |
|---|---|---|
| Large-scale cell culture | Primary cells, suspension culture at 2–4 mL | 48-well format with 5.0 mL volume suits scale-up culture programs |
| Organoid culture (high volume) | 3D organoid in Matrigel at 2–4 mL per well | 5.0 mL well volume for larger organoid embedding volumes |
| Protein expression (flask-scale) | Small-scale E. coli, yeast expression in 48-well | 5.0 mL deep well bridges 24-well and flask format expression |
| Virus propagation (large vol.) | Large-volume plaque assay, CPE assay | 5.0 mL per well for high-volume virus culture and titration |
| High-volume sample prep | Large-volume DNA extraction, protein purification | 5.0 mL eliminates need for tube-based large-volume prep |
| Bioprocess development | Parallel fermentation, cell culture optimization | 48-well 5.0 mL for high-throughput bioprocess screening |
| DMSO compound storage | Large-volume DMSO compound stocks, 48-plex | PPC DMSO compatibility for high-volume compound archive wells |
| Metabolite extraction | Large-volume metabolomics sample extraction | 5.0 mL accommodates extraction solvent volumes |
Working volume: up to ~4.5 mL per well. Tapered/chamfered bottom enables complete aspiration. PPC: DMSO + -80°C cryo archive available.
Chemical Resistance — Polypropylene Copolymer (PPC)
PPC: highest chemical resistance of deep-well materials — DMSO, -80°C cryo, and autoclave.
| Reagent / Condition | Compatibility |
|---|---|
| DMSO (all concentrations) | ✓ Excellent — superior to PP |
| Aqueous buffers | ✓ Excellent |
| Short-chain alcohols | ✓ Excellent |
| Acetonitrile / methanol | ✓ Excellent |
| Dilute acids / alkalies | ✓ Excellent |
| Chloroform / hexane (brief) | ✓ Good |
| Autoclave (121°C) | ✓ Compatible |
| Storage at -80°C | ✓ Suitable — key advantage over PP |
Laboratory Applications
Serves cell biology programs scaling up from 24-well to deep-well format; organoid culture programs requiring 2–4 mL per well; bioprocess development for parallel high-volume culture optimization; protein expression screening in deep-well format; large-volume metabolomics extraction; and any application bridging 24-well plate format and flask-scale volumes in 48-well SBS footprint.
U.S. Manufacturing, Quality & Institutional Procurement
Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.
Frequently Asked Questions — Caplugs® Evergreen
What is the advantage of 5.0 mL deep-well plates over standard 48-well plates?
The 5.0 mL well volume provides ~14× vs 24-well the capacity of standard 24-well format wells in the same SBS footprint. This enables: high-volume lysis buffer incubation per well without overflow; larger input volumes for DNA/RNA extraction protocols; complete protein precipitation with adequate organic:aqueous phase ratio; multi-step extraction and wash series in a single well; and per-well biobank aliquoting at higher volumes. The same SBS footprint maintains compatibility with existing automation infrastructure.
What makes 48-well 5.0 mL format different from 96-well deep well and 24-well plates?
The 48-well 5.0 mL format bridges the gap between 24-well plates (~3.5 mL per well) and deep well 96-well (≤2.0 mL per well). It provides 5.0 mL per well in 48 positions — more volume than any 96-well deep well, more wells than a 24-well plate, and the SBS-compatible footprint of standard 48-well format. Choose this format for applications requiring both large volume per well (>2.0 mL) and higher well count than 6- or 24-well plates.
Are the deep-well plates compatible with centrifugation?
Polypropylene Copolymer (PPC) deep-well plates in SBS footprint are compatible with standard centrifuge adapters for 48-well SBS plates. Typical use: 500–4,000 × g for pelleting and clarification. Confirm the g-force limit with your centrifuge rotor manufacturer before exceeding 2,000 × g — deep-well plate walls are thicker than standard plates but g-force limits depend on fill volume and rotor geometry.
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