0.1–0.45 mL Deep Well Microplates, PPC, Natural — 290-8525-010 | 5/Bag | Caplugs® Evergreen

96-Well Deep Well Microplates, Tapered Bottom, 0.5 mL Well Volume, Polystyrene (PS), Natural — 290-8525-010 | 5/Bag (20/Case)

Technical Narrative

The Caplugs® Evergreen is a 96-well deep well Polystyrene (PS) microplate with tapered bottom wells providing 0.5 mL per well — five times the capacity of standard 96-well plates — in the same SBS/ANSI standard footprint compatible with all standard 96-well plate readers, liquid handlers, and centrifuge adapters. Deep well plates address the most common volume constraint in 96-well sample preparation: the standard 96-well well volume (~0.37 mL) is insufficient for high-volume lysis buffer incubation, large-volume DNA extraction protocols, pre-PCR sample normalization, and biobank aliquoting workflows requiring >0.3 mL per well. The tapered bottom enables complete aspiration of well contents without residual volume loss at the well base.

PS construction provides excellent chemical resistance for standard aqueous sample preparation workflows — DNA/RNA extraction, protein digestion, cell lysis, and PCR normalization in aqueous buffers. PS deep well plates are the cost-effective format for high-volume sample prep programs where DMSO exposure and cryogenic storage are not required. For applications involving DMSO-containing sample matrices, organic solvent extraction, or -80°C long-term storage, the PPC variant (290-8538-080) provides the appropriate material specification.

At $83.72 per 5/Bag — $4.1856 per plate — the delivers deep well PS capacity at per-plate economics for high-volume sample prep programs. Also available: 290-8538-080 (PPC Natural).

Technical Specifications — Caplugs® Evergreen

96-Well Deep Well Plate Workflow Compatibility — PS 0.5 mL

96-well deep well plates provide 5× the volume of standard 96-well wells in the same SBS footprint. Key applications for the PS format:

Working volume is typically 0.1–0.45 mL per well — do not overfill above 0.5 mL max. Tapered bottom enables complete aspiration. PS: choose for standard aqueous sample prep at lower cost; upgrade to PPC (290-8538-080) for DMSO or cryo requirements.

Chemical Resistance — Polystyrene (PS)

PS resists standard aqueous reagents. Choose PPC (290-8538-080) for DMSO or cryo deep-well requirements.

Laboratory Applications

The serves genomics and molecular biology programs performing high-volume DNA/RNA extraction in 96-well format; proteomics programs running protein digestion and cleanup; clinical diagnostics for large-volume sample preparation; biorepository programs requiring high-volume per-well aliquoting; PCR pre-preparation and normalization workflows; and any application requiring 5× standard 96-well volume in a standard SBS footprint.

U.S. Manufacturing, Quality & Institutional Procurement

Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.

Frequently Asked Questions — Caplugs® Evergreen

What is the advantage of deep well plates over standard 96-well plates?

Deep well plates provide 0.5 mL per well versus ~0.37 mL in standard 96-well — approximately 5× more volume in the same SBS footprint. This eliminates the need to use multiple standard wells per sample, enables high-volume lysis (400+ µL lysis buffer per well), accommodates large-volume DNA extraction protocols, and provides sufficient working volume for pre-PCR normalization and dilution series in a single deep well rather than splitting across multiple standard wells.

When should I use PS deep well instead of PPC (290-8538-080)?

Choose PS for standard aqueous sample preparation — DNA/RNA extraction in aqueous buffers, protein digestion, PCR normalization — where DMSO, organic solvents, and -80°C long-term storage are not required. PS is the cost-effective default. Upgrade to PPC (290-8538-080) when DMSO-containing sample matrices, organic solvent extraction, or -80°C archive storage are workflow requirements.

Are the deep well plates BPA-free and compatible with centrifugation?

Yes — BPA-free, no phthalates. Polystyrene (PS) deep well plates are compatible with standard centrifuge adapters for 96-well SBS-footprint plates. Confirm the g-force rating with your centrifuge rotor manufacturer before spinning — deep well plate walls are thicker than standard plates but have specific g-force limits depending on sample volume and fill level. Typical use: 500–3,000 × g for pelleting and clarification.