0.112 mL Microplates, Flat Bottom, PS, Black, Thermo Fisher® Compatible — 290-8225-Z10 | 12/Box | Caplugs® Evergreen

384-Well Flat Bottom Microplates, 0.112 mL Well Volume, PS, Black — 290-8225-Z10 | 12/Box (60/Case)

Technical Narrative

The Caplugs® Evergreen is a 384-well flat-bottom black Polystyrene (PS) microplate — combining the 4× throughput advantage of 384-well format with the low-background fluorescence performance of black PS well walls. Black PS absorbs stray and reflected photons that create well-to-well cross-talk at the high well density of 384-well format — a more significant interference source than in 96-well format, where larger well spacing reduces optical cross-talk from adjacent wells. The delivers 5–20× lower fluorescence background versus natural or white 384-well plates for HTRF, TR-FRET, FRET, and fluorescence intensity assays at HTS scale.

Black 384-well is the industry standard format for HTRF (Homogeneous Time-Resolved FRET), LanthaScreen, and LANCE TR-FRET assays in pharmaceutical HTS — the low-background requirement for detecting the weak time-resolved donor fluorescence signal above plate autofluorescence is particularly critical at 384-well density where well cross-talk between adjacent high-signal wells is amplified. The flat bottom maintains consistent optical path length for quantitative fluorescence intensity reads and is compatible with all standard 384-well-capable plate readers in fluorescence mode (PHERAstar FSX, EnVision, Synergy Neo2, CLARIOstar).

At $182.92 per Box of 12 — $3.0486 per plate — the delivers black 384-well fluorescence HTS format at per-plate economics for programs running 50–500+ fluorescence plates per week. Also available: white 384-well (290-8221-W10) for luminescence HTS and natural 384-well (290-8217-010) for ELISA and qPCR.

Technical Specifications — Caplugs® Evergreen

384-Well Black Fluorescence Assay Compatibility

Black PS 384-well plates combine the 4× throughput advantage of 384-well format with the low-background fluorescence performance of black PS well walls. Compatible workflows:

Black 384-well is the HTS standard for fluorescence-based assays. For luminescence assays (luciferase, CTG), use white 384-well (290-8221-W10). For absorbance ELISA, use natural 384-well (290-8217-010). 384-well edge-well evaporation is significant — use EZ-Seal™ film or lid for incubations >1 hour at 37°C.

Chemical Resistance — Polystyrene (PS)

Black PS resists aqueous fluorescence assay reagents. Not autoclave-compatible.

Laboratory Applications

The serves pharmaceutical HTS programs running fluorescence-based compound library screening at 384-well scale; HTRF and TR-FRET drug discovery programs; FRET biosensor and molecular interaction assays at high throughput; high-content screening (HCS) programs requiring low-autofluorescence 384-well plates for immunofluorescence cell imaging; nucleic acid dye quantification at 384-well scale; and any fluorescence assay program scaling from 96-well black to 384-well black format.

U.S. Manufacturing, Quality & Institutional Procurement

Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.

Frequently Asked Questions — Caplugs® Evergreen

Is the black 384-well the correct format for HTRF assays?

Yes. Black PS 384-well plates are the recommended format for HTRF (Cisbio), LanthaScreen (Thermo Fisher), and LANCE (PerkinElmer) TR-FRET assays. Black background eliminates donor fluorescence leakage and plate autofluorescence that interfere with the time-resolved emission window detection. HTRF assay developers universally specify black plates because the TR-FRET ratio (665 nm / 620 nm) is highly sensitive to background fluorescence at 620 nm — any plate material fluorescence in this window directly inflates the denominator and collapses the assay window.

What is the difference between black , white (290-8221-W10), and natural (290-8217-010) 384-well plates?

All three are 384-well flat-bottom PS plates in SBS standard footprint. Color determines the optical interaction with photons: Black absorbs stray light — minimizes fluorescence background and well cross-talk for FI, FRET, HTRF assays. White (290-8221-W10) reflects photons — maximizes luminescence signal for luciferase, CellTiter-Glo, and chemiluminescent ELISA. Natural (290-8217-010) transmits light — standard for absorbance ELISA, qPCR, and fluorescence intensity where plate color has minimal effect on the measurement mode.

Is the compatible with 384-well high-content screening (HCS)?

Yes. Black 384-well plates are widely used for high-content screening (HCS) and high-content imaging (HCI) where plate autofluorescence and well cross-talk must be minimized for sensitive immunofluorescence detection. However, note that standard PS 384-well plates are not TC-treated — for demanding adherent cell lines used in HCS, TC-treated variants provide better cell attachment consistency for imaging uniformity across all 384 wells. Validate cell attachment with your specific cell line before committing to bulk purchase.