20–80 µL Microplates, Flat Bottom, PS, White — 290-8221-W10 | 12/Box | Caplugs® Evergreen

384-Well Flat Bottom Microplates, 0.112 mL Well Volume, PS, White — 290-8221-W10 | 12/Box (60/Case)

Technical Narrative

The Caplugs® Evergreen is a 384-well flat-bottom white Polystyrene (PS) microplate in SBS/ANSI standard footprint — delivering 4× assay throughput versus 96-well format at the same plate footprint and automation infrastructure. The 384-well format is the standard for pharmaceutical HTS programs where compound library screening at 96-well scale consumes prohibitive reagent volumes — shifting to 384-well reduces reagent cost per data point by approximately 75% while quadrupling the number of compounds screened per plate run. The 0.112 mL well volume suits working volumes of 20–80 µL per well, accommodating standard 384-well ELISA, HTS, and luminescence reporter assay volumes with consistent flat-bottom path length for well-bottom luminescence detection.

White PS maximizes luminescence signal by reflecting photons from well walls back toward the plate reader detector — providing 2–5× higher signal versus natural PS for luciferase reporter assays, NanoLuc, CellTiter-Glo, and ATP bioluminescence viability assays at 384-well HTS scale. White 384-well is the default plate format for pharmaceutical HTS luminescence reporter programs. All Caplugs® Evergreen 384-well plates conform to the SBS/ANSI 384-well standard footprint for full compatibility with 384-channel liquid handlers, 384-well plate readers, and high-density plate stacking systems.

At $182.92 per Box of 12 — $3.0486 per plate — the delivers 384-well white HTS format at per-plate economics for programs running 50–500+ plates per week. Also available: 290-8217-010 (384-well flat-bottom PS Natural, $182.92/box).

Technical Specifications — Caplugs® Evergreen

384-Well Assay Compatibility — White PS

384-well format provides 4× throughput versus 96-well at the same SBS footprint. White PS maximizes luminescence signal for HTS reporter and viability assays in 384-well format:

Working volume for 384-well is typically 20–80 µL per well. Use a multichannel pipette or robotic liquid handler for efficient 384-well dispensing. Edge-well evaporation is more significant at 384-well than 96-well — use a plate seal (290-8200-MIC) or lid for extended incubations.

Chemical Resistance — Polystyrene (PS)

PS resists aqueous assay reagents. Not autoclave-compatible.

Laboratory Applications

The serves pharmaceutical HTS programs scaling compound library screening to 384-well format; drug discovery programs running 384-well luciferase reporter screens; cell viability HTS programs using CellTiter-Glo and NanoLuc; kinase and enzyme assay programs using luminescence-based detection; and any HTS luminescence program where white plate signal enhancement at 384-well scale is the performance requirement.

U.S. Manufacturing, Quality & Institutional Procurement

Every Caplugs® Evergreen lot is manufactured at our California facility and subject to rigorous quality inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.

Frequently Asked Questions — Caplugs® Evergreen

What is the primary advantage of 384-well format over 96-well for HTS?

384-well format provides four key advantages over 96-well at the same SBS footprint: (1) 4× throughput — 384 compounds per plate vs 96; (2) 4× reagent savings — same assay volume distributed across 4× more wells; (3) Same automation infrastructure — 384-well plates fit all standard SBS-format liquid handlers, readers, and stacking systems; (4) Reduced consumable cost per data point. The trade-off is smaller well volumes (20–80 µL vs 100–300 µL) requiring more precise liquid handling and greater sensitivity to edge-well evaporation during extended incubations.

Is the white 384-well plate compatible with NanoLuc and CellTiter-Glo?

Yes. White 384-well plates are the recommended format for NanoLuc (Promega), CellTiter-Glo (CTG), CellTiter-Glo 2.0, and ATPlite luminescent viability assays at 384-well scale. White well walls reflect bioluminescence photons back toward the top-read detector, providing 2–5× higher signal versus natural PS — critical for NanoLuc assays where photon output is lower than standard luciferase and every reflected photon contributes to detection sensitivity.

Is the compatible with HTRF assays?

HTRF (Homogeneous Time-Resolved FRET) is typically run in white plates when luminescence signal enhancement is the priority, or black plates when cross-talk minimization is the priority. The white 384-well is compatible with HTRF readers (EnVision, PHERAstar, CLARIOstar) operating in TR-FRET mode. For HTRF assays with strong donor signal, white plate is standard; for weak signal with high background, consult your HTRF assay developer for plate color recommendation.