Caplugs® Evergreen
20–80 µL PCR Plates, Thin-Wall, PS, Natural — 290-8217-010 | 12/Box | Caplugs® Evergreen
20–80 µL PCR Plates, Thin-Wall, PS, Natural — 290-8217-010 | 12/Box | Caplugs® Evergreen
Couldn't load pickup availability
384-Well Flat Bottom Microplates, 0.112 mL Well Volume, PS, Natural — 290-8217-010 | 12/Box (60/Case)
| Quick Reference — 290-8217-010 | |
|---|---|
| Well Format | 384-well SBS/ANSI standard footprint |
| Bottom | Flat Bottom |
| Well Volume | 0.112 mL per well |
| Material | Polystyrene (PS) |
| Color | Natural |
| vs 96-well | 4× throughput, 4× reagent savings per plate vs 96-well |
| Primary Use | HTS, ELISA, qPCR, fluorescence intensity |
| Sterility | Non-Sterile |
| Pack Size | 12/Box (60/Case) |
| Price | $182.92 / Box ($3.0486 / plate) |
Technical Narrative
The Caplugs® Evergreen is a 384-well flat-bottom natural Polystyrene (PS) microplate in SBS/ANSI standard footprint — delivering 4× assay throughput versus 96-well format at the same plate footprint and automation infrastructure. The 384-well format is the standard for pharmaceutical HTS programs where compound library screening at 96-well scale consumes prohibitive reagent volumes — shifting to 384-well reduces reagent cost per data point by approximately 75% while quadrupling the number of compounds screened per plate run. The 0.112 mL well volume suits working volumes of 20–80 µL per well, accommodating standard 384-well ELISA, HTS, and qPCR assay volumes with consistent flat-bottom path length for absorbance and fluorescence reads.
Natural PS is the standard 384-well plate color for absorbance ELISA, fluorescence intensity, and qPCR applications where plate color has minimal impact on transmission-mode reads or where transparent PS optical properties are required for the detection mode. The flat bottom ensures consistent path length for absorbance ELISA at 450 nm, 405 nm, and 490 nm across all 384 wells — a critical requirement when scaling ELISA from 96-well to 384-well format. All Caplugs® Evergreen 384-well plates conform to the SBS/ANSI 384-well standard footprint for full compatibility with 384-channel liquid handlers, 384-well plate readers, and high-density plate stacking systems.
At $182.92 per Box of 12 — $3.0486 per plate — the delivers 384-well natural HTS format at per-plate economics for programs running 50–500+ plates per week. Also available: 290-8221-W10 (384-well flat-bottom PS White, $182.92/box).
Technical Specifications — Caplugs® Evergreen
| Specification | Value |
|---|---|
| Part Number | |
| Caplugs Item # | 2908217010E1 |
| Well Format | 384-well SBS/ANSI standard footprint |
| Bottom | Flat Bottom |
| Well Volume | 0.112 mL (working volume typically 20–80 µL) |
| Well Diameter | ~3.7 mm inner diameter |
| Material | Polystyrene (PS) |
| Color | Natural |
| Sterility | Non-Sterile |
| Pack Size | 12/Box (60/Case) |
| Application | HTS, ELISA, fluorescence intensity, qPCR |
| BPA/Phthalate | BPA-free; no phthalate plasticizers |
| Country of Origin | Made in U.S.A. (California) |
384-Well Assay Compatibility — Natural PS
384-well format provides 4× throughput versus 96-well at the same SBS footprint. Natural PS is the standard for absorbance-based ELISA, fluorescence intensity, and qPCR in 384-well format:
| Application | Assay / Use | Notes |
|---|---|---|
| High-throughput screening (HTS) | Small molecule compound screening at 384-well scale | 384-well reduces reagent use 4× vs 96-well for same compound count |
| ELISA (384-well format) | 384-well ELISA for high-throughput serology | Flat bottom for absorbance reads; 4× throughput vs 96-well ELISA |
| qPCR / RT-PCR detection | 384-well quantitative PCR plates | Natural PS: pair with optical adhesive seal for real-time PCR |
| Cell-based HTS | Compound cytotoxicity, proliferation screening | 384-well cell culture requires TC-treated variant for adherent cells |
| Kinase / enzyme assay panels | Fluorescence or luminescence kinase screening | 384-well format reduces enzyme and substrate costs per data point |
| Lead optimization panels | IC50/EC50 determination at 384-well scale | Natural PS suits absorbance and fluorescence intensity modes |
| Sample dilution plates | Serial dilution source plates for HTS | 384-well format for pre-diluted compound series delivery |
| DNA/RNA quantification arrays | Fluorescent dye nucleic acid 384-well panels | Natural PS for fluorescence intensity at 384-well throughput |
Working volume for 384-well is typically 20–80 µL per well. Use a multichannel pipette or robotic liquid handler for efficient 384-well dispensing. Edge-well evaporation is more significant at 384-well than 96-well — use a plate seal (290-8200-MIC) or lid for extended incubations.
Chemical Resistance — Polystyrene (PS)
PS resists aqueous assay reagents. Not autoclave-compatible.
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers | ✓ Excellent |
| Cell culture media | ✓ Excellent |
| DMSO (≤10%) | ✓ Good |
| Short-chain alcohols (brief) | ✓ Good |
| Formalin (4%, brief) | ✓ Good |
| Acetone | ✗ Avoid |
| Autoclave | ✗ Not compatible |
Laboratory Applications
The serves pharmaceutical HTS programs scaling compound library screening to 384-well format; academic and CRO labs running 384-well ELISA and qPCR for high-throughput sample processing; genomics programs running 384-well nucleic acid quantification panels; cell biology labs running 384-well dose-response assays for IC50/EC50 determination; and any assay program requiring 4× throughput improvement over 96-well at the same automation infrastructure footprint.
U.S. Manufacturing, Quality & Institutional Procurement
Every Caplugs® Evergreen lot is manufactured at our California facility and subject to rigorous quality inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.
Frequently Asked Questions — Caplugs® Evergreen
What is the primary advantage of 384-well format over 96-well for HTS?
384-well format provides four key advantages over 96-well at the same SBS footprint: (1) 4× throughput — 384 compounds per plate vs 96; (2) 4× reagent savings — same assay volume distributed across 4× more wells; (3) Same automation infrastructure — 384-well plates fit all standard SBS-format liquid handlers, readers, and stacking systems; (4) Reduced consumable cost per data point. The trade-off is smaller well volumes (20–80 µL vs 100–300 µL) requiring more precise liquid handling and greater sensitivity to edge-well evaporation during extended incubations.
Is the natural PS 384-well plate compatible with qPCR?
Natural PS 384-well plates are compatible with qPCR detection when paired with a clear optical adhesive film seal (EZ-Seal™ 290-8200-MIC). Natural PS transmits excitation and emission wavelengths for standard qPCR dyes (FAM, SYBR, ROX) in top-read fluorescence mode. For dedicated PCR applications, thin-wall PCR-specific 384-well plates optimize thermal transfer — confirm that the wall thickness meets your instrument's thermal ramp specification.
Is the plate reader-compatible for ELISA absorbance?
Yes. The conforms to the SBS/ANSI 384-well standard footprint and flat bottom, ensuring compatibility with all standard 384-well-capable plate readers including SpectraMax (with 384-well adapter), PHERAstar FSX, Synergy Neo2, Infinite M1000 Pro, and EnVision. Flat bottom ensures consistent OD path length for absorbance-mode ELISA across all 384 well positions.
Share
