0.37 mL Round-Bottom 8-Well PS Strips, Natural — Caplugs® Evergreen Partial-Plate ELISA & Agglutination Strips (290-8172-01R)
These natural (transparent) polystyrene 8-well strips feature a 0.37 mL round-bottom (U-bottom) well geometry designed for partial-plate ELISA, suspension cell culture, hemagglutination inhibition (HAI), and serial dilution workflows. Supplied 50 strips per bag (400 wells total), part number 290-8172-01R seats in standard 12-position SBS strip frames — including the compatible Caplugs® Evergreen 290-8103-Z1N HIPS black frame — to reconstitute a full 96-position SBS footprint for use with automated washers, plate readers, and robotic handlers. The round-bottom geometry allows cells and particles to settle by gravity into a uniform central pellet, matching the optical and physical behavior of a full round-bottom 96-well plate at strip-format economics.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 290-8172-01R |
| Format | 8-Well Strip |
| Well Bottom | Round (U-Bottom) |
| Well Volume | 0.37 mL |
| Material | Polystyrene (PS) |
| Color | Natural (Transparent) |
| Strip Frame Compatibility | Caplugs® Evergreen 290-8103-Z1N HIPS Black Frame; standard 12-position SBS strip frames |
| Sterility | Non-Sterile |
| Pack Size | 50 Strips/Bag (400 Wells Total) |
| BPA / Phthalates | BPA-Free; No Phthalate Plasticizers |
| Country of Origin | USA |
Applications
- Partial-plate ELISA: Load only the strips required for each run; return unused antibody-coated strips to cold storage and eliminate per-plate waste on unneeded wells.
- Hemagglutination inhibition (HAI) assays: Round-bottom geometry is the WHO standard for RBC button scoring in HAI and viral titer assays; strip format allows sample-count-matched loading without fixed plate constraints.
- Suspension cell culture: Lymphocyte, hybridoma, and PBMC culture benefit from U-bottom geometry that settles cells into a uniform central pellet for consistent growth and harvest.
- Immunoassay serology (partial plate): Allergen-specific IgE, agglutination, and strip-format serology workflows that require fewer than a full plate of wells per run.
- Serial dilution: Eight-step, two-fold dilution series per strip — ideal for dose-response titrations and antibody dilution panels.
- Cell-based luminescence: Luciferase reporter assays in suspension cells using strip-format throughput with round-bottom settling geometry.
Compatibility
These 8-well round-bottom strips are designed to seat in standard 12-position SBS strip frames, assembling a full 96-well SBS footprint compatible with most microplate washers, absorbance readers, and liquid-handling robots. The Caplugs® Evergreen 290-8103-Z1N HIPS black strip frame is a confirmed compatible frame for creating complete 96-well assemblies. When specifying a strip-frame combination for an automated instrument, verify that the assembled plate height and footprint meet your instrument's deck specifications.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers (PBS, TBS, carbonate coating buffers) | Excellent |
| Cell culture media (RPMI, DMEM, MEM) | Excellent |
| Dilute detergents (Tween-20, Triton X-100 at assay concentrations) | Good — short-term contact at working dilutions |
| Ethanol >70% | Not recommended |
| Acetone / chlorinated solvents | Not recommended |
| Aromatic solvents (toluene, xylene) | Not recommended |
| Autoclaving | Not suitable — PS is not autoclavable |
Quality & Procurement
Made in USA, these Caplugs® Evergreen strips are manufactured from BPA-free, phthalate-free polystyrene resin under a quality management system consistent with ISO manufacturing standards. LabSupplies.com is an authorized Caplugs® Evergreen dealer shipping from USA inventory; stocking items typically ship same business day, and tiered pricing is available for high-volume, institutional, and grant-funded laboratory accounts.
Frequently Asked Questions
Can these strips be used in a standard 96-well plate reader without a frame?
The strips require a 12-position SBS strip frame — such as the compatible Caplugs® Evergreen 290-8103-Z1N frame — to achieve the standard 96-well SBS footprint that plate readers, washers, and robotic handlers expect. Individual strips alone do not present the correct dimensions for most automated instruments.
Are these strips sterile?
No — they are supplied non-sterile. For cell culture applications that require a sterile field, handle the strips using aseptic technique in a biosafety cabinet and consider UV or gamma-irradiated alternatives if your protocol demands certified sterility.
Why is round-bottom geometry important for HAI assays?
The World Health Organization protocol for hemagglutination inhibition (HAI) specifies round-bottom (U-bottom) wells because the curved floor allows red blood cells to settle into a compact, easily scored button under gravity. Flat-bottom wells produce a dispersed RBC monolayer that is difficult to distinguish from a non-inhibited pattern, making round-bottom geometry essential for accurate titer endpoint reading.
How does strip format reduce reagent waste compared to full plates?
Because each strip holds only 8 wells, labs can load exactly as many strips as they have samples for a given run. Unused antibody-coated strips can be sealed and returned to cold storage rather than discarded, which is particularly valuable when coating reagents — such as viral antigens or expensive allergen extracts — represent a significant per-well cost.