Caplugs® Evergreen
0.30 mL Microplates, PS, Natural — 290-8116-01V | 10/Box | Caplugs® Evergreen
0.30 mL Microplates, PS, Natural — 290-8116-01V | 10/Box | Caplugs® Evergreen
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96-Well Conical (V-Bottom) Bottom Microplates, 0.30 mL Well Volume, PS, Natural — 290-8116-01V | 10/Box (100/Case)
| Quick Reference — 290-8116-01V | |
|---|---|
| Well Format | 96-well SBS/ANSI standard footprint |
| Bottom Geometry | Conical (V-Bottom) Bottom |
| Well Volume | 0.30 mL |
| Material | Polystyrene (PS) |
| Color | Natural |
| Primary Use | Complete cell and particle pelleting |
| Sterility | Non-Sterile |
| Pack Size | 10/Box (100/Case) |
| Price | $120.33 / Box ($1.2033 / plate) |
Technical Narrative
The Caplugs® Evergreen is a 96-well conical (v-bottom) bottom Polystyrene (PS) microplate in natural for complete cell and particle pelleting, supernatant removal, serial dilution, and applications requiring maximum sample recovery from the well bottom. The V-bottom apex concentrates the entire cell or bead pellet at a single geometric point — enabling complete supernatant removal with a single aspirate pass and maximum sample recovery from minimal residual volume. Natural (clear) PS — V-bottom geometry is the critical feature for these applications. Complete pelleting and supernatant removal depend on the apex geometry, not well color.
All 96-well Caplugs® Evergreen microplates conform to the SBS/ANSI microplate standard footprint (127.76 × 85.47 mm), ensuring compatibility with all standard plate readers (SpectraMax, PHERAstar, Synergy, Infinite, EnVision), automated liquid handlers (Hamilton, Tecan, Bravo), plate washers, and robotic gripper arm systems. The 0.30 mL well volume accommodates standard assay reagent addition sequences across the primary applications for conical (v-bottom) format plates. PS construction is compatible with all standard aqueous assay buffers, culture media, and detection reagents.
At $120.33 per Box of 10 — $1.2033 per plate — the delivers conical (v-bottom) PS microplate economics for programs running 10–200+ plates per week. The 10/Box (100/Case) format supports both per-box and case-quantity ordering.
Technical Specifications — Caplugs® Evergreen
| Specification | Value |
|---|---|
| Part Number | |
| Caplugs Item # | 290811601VE1 |
| Well Format | 96-well SBS/ANSI standard footprint |
| Bottom Geometry | Conical (V-Bottom) Bottom |
| Well Volume | 0.30 mL (working volume typically 50–80% of max) |
| Material | Polystyrene (PS) |
| Color | Natural |
| Sterility | Non-Sterile |
| Pack Size | 10/Box (100/Case) |
| Application | Complete cell and particle pelleting |
| BPA / Phthalate | BPA-free; no phthalate plasticizers |
| Country of Origin | Made in U.S.A. (California) |
Assay & Application Compatibility — Conical (V-Bottom) Bottom, Natural
Conical (V-Bottom) bottom PS natural plates are the standard format for complete cell and particle pelleting, supernatant removal, serial dilution, and applications requiring maximum sample recovery from the well bottom. Compatible workflows:
| Application | Assay / Use | Notes |
|---|---|---|
| Complete cell pelleting | Any cell type requiring 100% pellet recovery | V-bottom concentrates pellet at exact well apex for minimal loss |
| Supernatant harvest assays | Cytokine quantification from cell supernatants | Sharp V-apex enables complete supernatant removal without pellet disturbance |
| Serial dilution (2-fold, 10-fold) | Titration, dose-response, viral dilution | V-bottom ensures complete mixing at small volumes during dilution |
| Magnetic bead assays | Bead-based immunoassay, MACS depletion | V-bottom focuses bead pellet for efficient supernatant aspirate |
| Red blood cell (RBC) assays | Blood typing, crossmatch, DAT | V-bottom is the standard format for RBC agglutination and button assays |
| Compound dilution (drug discovery) | Source plate for acoustic dispensing, pintool | V-bottom maximizes compound recovery from small dispensed volumes |
| Proteomics sample prep | In-solution digest, peptide cleanup | V-bottom for complete pellet recovery in protein prep workflows |
| Worm / organism assay | C. elegans, zebrafish larvae culture | V-bottom centers organisms for imaging and scoring |
Natural (clear) PS — V-bottom geometry is the critical feature for these applications. Complete pelleting and supernatant removal depend on the apex geometry, not well color.
Chemical Resistance — Polystyrene (PS)
PS provides excellent resistance to aqueous assay reagents. Not autoclave-compatible. Avoid acetone and chlorinated solvents.
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers (PBS, TBS, HEPES) | ✓ Excellent |
| Cell culture media (DMEM, RPMI) | ✓ Excellent |
| DMSO (≤10%) | ✓ Good |
| Short-chain alcohols (brief) | ✓ Good |
| Dilute acids / alkalies (<10%) | ✓ Good |
| Formalin (4%, fixation step) | ✓ Good |
| Acetone | ✗ Avoid |
| Chlorinated solvents | ✗ Avoid |
| Autoclave (121°C) | ✗ Not compatible |
Laboratory Applications
The V-bottom plate serves cell biology programs requiring complete cell pelleting and supernatant harvest; blood banking labs running ABO/Rh blood typing and crossmatch; drug discovery compound dilution and acoustic dispensing source plates; proteomics sample prep; magnetic bead immunoassay programs; and any workflow requiring 100% sample recovery from the well bottom.
U.S. Manufacturing, Quality & Institutional Procurement
Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.
Frequently Asked Questions — Caplugs® Evergreen
When should I choose V-bottom over U-bottom for cell pelleting?
V-bottom (conical) concentrates the entire cell or particle pellet at the single geometric apex of the well — enabling 100% pellet recovery with minimal residual volume. U-bottom collects cells in a broader curved bowl — better for suspension culture where cells need to re-distribute uniformly, but leaving more cells at the well edges during aspiration. Choose V-bottom when maximum pellet recovery and complete supernatant removal are required — blood typing, compound dilution source plates, proteomics pellets, and magnetic bead assays. Choose U-bottom for cell culture and agglutination visualization.
Is the used for blood typing and crossmatch assays?
Yes. V-bottom 96-well plates are the standard format for RBC blood typing (ABO/Rh), antibody screening, crossmatch, and direct antiglobulin testing (DAT) in blood bank gel-card and tube testing workflows. The V-bottom concentrates the RBC pellet at the apex for clear agglutination vs no-agglutination visual scoring. Confirm format with your specific blood bank method validation before adopting for regulated diagnostic use.
Is the compatible with acoustic dispensers?
Yes. V-bottom 96-well plates in SBS footprint are compatible with acoustic liquid dispensers (Labcyte Echo, SPT Labtech Mosquito) as compound source plates. The V-bottom geometry maximizes compound recovery from small dispensed volumes (100 nL–10 µL range) — the acoustic transducer can eject from the well apex with minimal dead volume. Confirm plate specifications against your dispenser's source plate requirements.
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