Bottle Top Filter PES 0.22μm 150-1000mL Sterile | Globe Scientific

Engineered for rapid sterile filtration of cell culture media and biological solutions, this bottle top filter features a 0.22μm PES membrane across four volume capacities. Polyethersulfone delivers the highest flow rates among hydrophilic membranes while maintaining very low protein binding — critical for preserving growth factor activity in sensitive media formulations. Each unit threads directly onto standard 45mm neck bottles with an integrated hose barb for vacuum line connection.

Bottle Top Filter Features and Benefits

• Membrane: PES (Polyethersulfone) — 0.22μm pore size, hydrophilic, very low protein binding
• Volumes: 150mL, 250mL, 500mL, and 1000mL — select from the options above
• Sterility: Gamma irradiated to SAL 10⁻⁶, individually wrapped, certified non-pyrogenic
• Fit: Standard 45mm bottle neck thread for universal glass and plastic bottle compatibility
• Connection: Integrated hose barb for vacuum line — no adapters or fittings required
• Identification: Membrane type and pore size printed on each unit for rapid selection

Available Sizes and Pack Quantities

Laboratory Applications

• Sterilization-grade filtration of cell culture media, serum, and growth supplements
• Buffer and reagent sterilization for molecular biology and protein purification
• Preparation of sterile solutions inside laminar flow hoods and biosafety cabinets
• Filtration of injectable preparations and diagnostic reagent solutions
• Removal of particulates from HPLC mobile phases and chromatography buffers

Quality Assurance and Compliance

Globe Scientific Diamond PureFlow bottle top filters are manufactured in ISO-certified cleanroom facilities and gamma sterilized to SAL 10⁻⁶. Each PES membrane undergoes bubble point integrity testing before assembly to verify pore size specification. Units are individually wrapped and lot-number traceable for quality documentation in GLP, GMP, and ISO 17025 accredited environments. PES membrane meets USP Class VI biocompatibility requirements.

Bottle Top Filter Comparison: Why Choose Globe Scientific?

Volume Discounts and Institutional Pricing

LabSupplies.com offers tiered quantity pricing for academic and research institutions. As an authorized dealer, we ship directly from USA inventory.

Frequently Asked Questions

What pore size bottle top filter should I use for sterilization?

A 0.22μm pore size is the industry standard for sterilization-grade filtration, effectively removing bacteria and microorganisms from liquid media. Use 0.45μm for general clarification and pre-filtration where sterility is not the primary objective. Globe Scientific PureFlow filters are available in both pore sizes across all volume capacities.

Are PES bottle top filters compatible with serum and protein media?

PES membranes provide very low protein binding, making them ideal for filtering serum-containing media without losing growth factors or cytokines. Compared to cellulose acetate alternatives, PES delivers faster flow rates and higher protein recovery. All four volumes use identical PES membrane chemistry.

What volumes are available for PureFlow bottle top filters?

Globe Scientific Diamond PureFlow 0.22μm bottle top filters are available in 150mL (VF-BT-PES-0150-22), 250mL (VF-BT-PES-0250-22), 500mL (VF-BT-PES-0500-22), and 1000mL (VF-BT-PES-1000-22). Each size ships in cases of 24 individually wrapped sterile units from LabSupplies.com at below retail pricing.