24-Well Flat-Bottom Microplates with Lids — Sterile, 3.5 mL/Well, Polystyrene, Natural | 100/Bag (222-8044-01F)
These sterile 24-well flat-bottom polystyrene microplates deliver 3.5 mL per well in the standard SBS footprint, each plate individually packed with a lid for point-of-use aseptic technique. The flat-bottom well geometry provides a uniform optical path length for absorbance and fluorescence microplate reader measurements, while the included lid controls evaporation during extended incubations and reduces contamination risk. Supplied 100 plates per bag, part number 222-8044-01F is well suited to cell culture, immunoassay, and any workflow requiring a larger well volume than the 96-well format.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-8044-01F |
| Well Format | 24-Well |
| Well Volume | 3.5 mL |
| Bottom Style | Flat Bottom |
| Lid Included | Yes |
| Material | Polystyrene (PS) |
| Color | Natural (Clear) |
| Sterility | Sterile |
| Pack Size | 100 / Individually Packed |
| BPA / Phthalate Free | Yes |
| Country of Origin | USA |
Applications
- ELISA and colorimetric immunoassays requiring a flat optical surface for uniform absorbance path length
- Fluorescence-based microplate reader assays benefiting from PS optical clarity
- Cell culture, cytotoxicity assays, and transfection optimization where the 3.5 mL well volume provides more growth area than a 96-well plate
- Assay workflows using standard single-channel or multichannel pipettes with the standard 24-well spacing
- Automated liquid handling and microplate washer protocols on SBS-footprint instrumentation
- Extended incubations where the included lid minimizes evaporation and reduces contamination risk
- CAP-, CLIA-, and ISO 15189-regulated laboratories requiring individually packed, lot-traceable consumables for aseptic technique
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline | Excellent |
| Dilute acids | Excellent |
| Dilute bases | Excellent |
| Methanol | Good — short-term contact |
| Acetonitrile | Caution — limited compatibility; verify for your application |
| Ethyl acetate | Not recommended |
| Aromatic / chlorinated solvents | Not recommended |
Quality & Procurement
Made in USA, these Caplugs® Evergreen microplates are manufactured at ISO-certified facilities from BPA-free, phthalate-free polystyrene resin. Each plate and lid are individually packed sterile, supporting aseptic technique at point of use and meeting the lot-traceability requirements of CAP, CLIA, and ISO 15189 accredited laboratories. LabSupplies.com is an authorized dealer of Caplugs® Evergreen consumables and ships from USA inventory, with tiered pricing available for high-volume and grant-funded accounts.
Frequently Asked Questions
Are these microplates sterile?
Yes — each plate and its lid are individually packed and supplied sterile, making them suitable for aseptic cell culture and sensitive assay workflows directly out of the packaging.
Do these plates fit standard microplate readers and automated liquid handlers?
Yes. The plates conform to a standard SBS footprint, making them compatible with most microplate readers, washers, and automated liquid handling systems designed for 24-well format. The flat-bottom wells provide the uniform optical path length required for absorbance and fluorescence measurements.
Why choose a 24-well plate over a 96-well format?
The 3.5 mL per-well volume accommodates workflows that require a larger volume of culture media, reagent, or sample than a 96-well plate can hold, such as cell proliferation studies, cytotoxicity assays, and transfection optimization experiments.
Can polystyrene plates be used with organic solvents?
PS is well suited to aqueous buffers, dilute acids and bases, and short-term methanol contact, but it is not compatible with ethyl acetate, aromatic solvents, or chlorinated solvents. For solvent-heavy workflows, a polypropylene plate would be a more appropriate choice.