1.0 mL Deep Well Microplates, Chamfered, PPC, Natural — 222-8664-08N | 5/Bag | Caplugs® Evergreen

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Technical Narrative

The Caplugs® Evergreen 222-8664-08N is a 96-Well Deep Well PPC Microplate, Tapered Bottom, 1.0 mL per well, Sterile, 5/Bag. The 1.0 mL well volume is the standard deep well volume for most LC-MS sample preparation workflows — accommodating the full sample volume plus precipitation or extraction solvent at standard protocol ratios (1:2 or 1:3 sample:solvent). The tapered well bottom narrows to a smaller-diameter base — concentrating the sample at the well tip for more complete aspiration of small residual volumes after extraction or centrifugation.

PPC provides the critical material advantage for deep well LC-MS sample preparation: full compatibility with acetonitrile, methanol, ethyl acetate, and other organic extraction solvents used in protein precipitation, liquid-liquid extraction (LLE), and SPE elution steps that would crack or swell standard PS deep well plates under prolonged organic solvent exposure. BPA-free, phthalate-free, FDA-compliant,

Procurement economics: $93.73/Bag (5 plates), $18.7450/plate. Siblings: 222-8662-01N (1.0 mL PS Tapered Sterile 5/Bag); 222-8668-08C (2.0 mL PPC Chamfered Sterile 5/Bag); 222-8649-080 (0.5 mL PPC Tapered Non-Sterile 5/Bag). LabSupplies.com ships from USA inventory, typically same business day.

Technical Specifications

96-Well Deep Well Microplate (Tapered, 1.0 mL, PPC) Applications

The is compatible with the following sample preparation and high-throughput workflows.

Chemical Resistance — Polypropylene Copolymer (PPC) Deep Well Microplate

Compatibility for PPC — key organic solvent compatibility differences between PS and PPC are highlighted.

Clinical & Research Laboratory Applications

Caplugs® Evergreen 1.0 mL tapered-bottom PPC deep well microplates serve bioanalytical, DMPK, forensic toxicology, and clinical chemistry laboratories performing LC-MS/MS sample preparation in automated 96-well format. PPC is the material of choice for deep well LC-MS sample prep because its full organic solvent compatibility withstands acetonitrile and methanol protein precipitation, organic phase extraction in LLE, and SPE organic eluent collection without the cracking and analyte contamination risk that occurs when acetonitrile contacts PS deep well plates. Bioanalytical laboratories performing drug and metabolite quantitation in plasma by LC-MS/MS use 96-well PPC deep well plates as the primary sample preparation vessel in protein precipitation workflows: plasma is dispensed into the deep well plate, acetonitrile is added at a 2:1 ratio, the plate is mixed and centrifuged, and the supernatant is transferred directly from the deep well plate to a 96-well injection plate for LC-MS analysis.

U.S. Manufacturing, Quality & Institutional Procurement

Caplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.

LabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can request a written quote or speak with a product specialist directly from our sales engineering team.

Frequently Asked Questions

Is the deep well microplate Sterile?

Yes, sterile — packed 5/Bag.

What is the chemical compatibility advantage of PPC for LC-MS sample prep?

PPC provides full compatibility with acetonitrile, methanol, and ethyl acetate — the primary extraction and precipitation solvents in LC-MS sample preparation. PS deep well plates show crazing and potential analyte contamination with prolonged acetonitrile contact (protein precipitation) and are not suitable for ethyl acetate-based LLE. PPC eliminates this risk. Choose PS when your workflow uses only aqueous reagents or brief organic contact (< 30 min acetonitrile), and PPC for any protocol with extended organic solvent contact.

What is the difference between Tapered and Chamfered (222-8668-08C) deep well geometries?

Tapered (conical tip): narrower base concentrates the sample volume at the well tip for maximum recovery of small residual volumes after centrifugation — important for maximizing sample recovery in protein precipitation and LLE. Chamfered (beveled angle): angled well bottom guides the final liquid to the center of the well base for complete liquid handler tip aspiration without residual corners — preferred on automation platforms where the robotic tip needs to aspirate the well to near-dryness. The 2.0 mL chamfered well also provides more volume for higher-ratio extraction protocols.