Caplugs® Evergreen
1.0 mL Deep Well Microplates, Chamfered, PPC, Natural — 222-8662-01N | 5/Bag | Caplugs® Evergreen
1.0 mL Deep Well Microplates, Chamfered, PPC, Natural — 222-8662-01N | 5/Bag | Caplugs® Evergreen
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Quick Reference
| Part Number | 222-8662-01N |
|---|---|
| Format | 96-well deep well microplate |
| Well Bottom | Tapered |
| Well Geometry | Tapered conical well — concentrates sample at base for efficient aspiration |
| Well Volume | 1.0 mL (1000 µL) per well |
| Material | Polystyrene (PS) |
| Color | Natural |
| Sterility | Sterile |
| Pack Size | 5/Bag |
| Primary Use | Sample prep, compound storage, automated extraction |
| Origin | Made in USA |
| Price | $93.73/Bag ($18.7450/plate) |
Technical Narrative
The Caplugs® Evergreen 222-8662-01N is a 96-Well Deep Well PS Microplate, Tapered Bottom, 1.0 mL per well, Sterile, 5/Bag. The 1.0 mL well volume is the standard deep well volume for most LC-MS sample preparation workflows — accommodating the full sample volume plus precipitation or extraction solvent at standard protocol ratios (1:2 or 1:3 sample:solvent). The tapered well bottom narrows to a smaller-diameter base — concentrating the sample at the well tip for more complete aspiration of small residual volumes after extraction or centrifugation.
PS provides optical clarity for visual monitoring of well fill level and is compatible with aqueous and mildly organic sample preparation. Specify PS for aqueous extraction, protein precipitation with acetonitrile where brief contact is acceptable. BPA-free, phthalate-free, FDA-compliant,
Procurement economics: $93.73/Bag (5 plates), $18.7450/plate. Siblings: 222-8664-08N (1.0 mL PPC Tapered Sterile 5/Bag); 222-8636-010 (0.5 mL PS Tapered Sterile 5/Bag); 222-8668-08C (2.0 mL PPC Chamfered Sterile 5/Bag). LabSupplies.com ships from USA inventory, typically same business day.
Technical Specifications
| Part Number | |
|---|---|
| Caplugs Item # | |
| Format | 96-well deep well microplate |
| Well Bottom | Tapered |
| Well Volume | 1.0 mL (1000 µL) per well |
| Material | Polystyrene (PS) |
| Color | Natural |
| Sterility | Sterile |
| Pack Size | 5/Bag |
| Solvent Compatibility | Aqueous and brief ACN/MeOH contact (PS) |
| Application | LC-MS sample prep, SPE, LLE, protein precipitation, compound storage |
| BPA / Phthalate | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
| Price | $93.73/Bag ($18.7450/plate) |
96-Well Deep Well Microplate (Tapered, 1.0 mL, PS) Applications
The is compatible with the following sample preparation and high-throughput workflows.
| Application | Setting / Configuration | Notes |
|---|---|---|
| Protein precipitation (LC-MS) | Add ACN/MeOH to plasma, centrifuge, aspirate | PS: brief ACN contact acceptable |
| Liquid-liquid extraction (LLE) | Add organic extractant, mix, phase separate | 1.0 mL: sufficient for sample + extractant at standard LLE ratios |
| SPE elution collection | Collect SPE eluate in deep well plate | PS: aqueous eluent compatible |
| Compound library storage | DMSO compound stocks, —20°C or —80°C | Deep well for higher-volume compound stocks per well |
| Automated liquid handling | Robot-compatible SBS footprint | Tapered bottom: designed for liquid handler tip access |
| Cell-based prep (large volume) | Cell lysis, extraction, 96-well scale | 1.0 mL accommodates lysis volume + supernatant |
| Dried blood spot (DBS) elution | DBS punch elution into deep well | Deep well accommodates elution volume for DBS extraction |
Chemical Resistance — Polystyrene (PS) Deep Well Microplate
Compatibility for PS — key organic solvent compatibility differences between PS and PPC are highlighted.
| Reagent / Condition | Compatibility | Notes |
|---|---|---|
| Aqueous buffers / plasma | Excellent | PS compatible across pH 4–10. |
| Acetonitrile (<100%) | Limited | Brief contact acceptable; avoid extended immersion — PS may craze. |
| Methanol (<100%) | Limited | Short-term compatible; avoid prolonged contact. |
| DMSO (<5%) | Good | Compatible at standard compound concentrations. |
| Ethyl acetate | Not Compatible | Causes PS crazing; use PPC for LLE. |
| Aqueous acid/base (<10%) | Good | Compatible with dilute acid/base mobile phases. |
| Cold storage (—80°C) | Limited | PS more brittle at cryogenic temperatures vs PPC. |
| Acetone / chloroform | Not Compatible | Dissolves PS; do not use. |
Clinical & Research Laboratory Applications
Caplugs® Evergreen 1.0 mL tapered-bottom PS deep well microplates serve laboratory automation, compound management, and sample preparation workflows requiring a cost-effective PS deep well format for aqueous and mildly organic sample types. Compound library management uses PS deep well plates for DMSO compound stock storage and daughter plate generation where the DMSO concentration is low enough for PS compatibility. High-throughput screening laboratories use PS deep well plates for aqueous sample pooling, buffer transfer, and compound dilution steps where organic solvent contact is minimal. For strong organic solvent workflows (protein precipitation with acetonitrile, LLE with ethyl acetate), the PPC alternatives (222-8649-080 for 0.5 mL non-sterile, 222-8664-08N for 1.0 mL sterile) provide better material compatibility.
U.S. Manufacturing, Quality & Institutional Procurement
Caplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.
LabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can request a written quote or speak with a product specialist directly from our sales engineering team.
Frequently Asked Questions
Is the deep well microplate Sterile?
Yes, sterile — packed 5/Bag.
Why choose PPC over PS for deep well plates?
PPC provides full compatibility with acetonitrile, methanol, and ethyl acetate — the primary extraction and precipitation solvents in LC-MS sample preparation. PS deep well plates show crazing and potential analyte contamination with prolonged acetonitrile contact (protein precipitation) and are not suitable for ethyl acetate-based LLE. PPC eliminates this risk. Choose PS when your workflow uses only aqueous reagents or brief organic contact (< 30 min acetonitrile), and PPC for any protocol with extended organic solvent contact.
What is the difference between Tapered and Chamfered (222-8668-08C) deep well geometries?
Tapered (conical tip): narrower base concentrates the sample volume at the well tip for maximum recovery of small residual volumes after centrifugation — important for maximizing sample recovery in protein precipitation and LLE. Chamfered (beveled angle): angled well bottom guides the final liquid to the center of the well base for complete liquid handler tip aspiration without residual corners — preferred on automation platforms where the robotic tip needs to aspirate the well to near-dryness. The 2.0 mL chamfered well also provides more volume for higher-ratio extraction protocols.
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