100–200 µL Deep Well Microplates, Chamfered, PPC, Natural — 222-8636-010 | 5/Bag | Caplugs® Evergreen

96-Well Deep Well Microplate — 0.5 mL Tapered, Polystyrene, Sterile (222-8636-010)

These sterile 96-well deep well microplates feature a tapered conical well bottom and 0.5 mL (500 µL) per-well volume, making them a precise fit for miniaturized high-throughput sample preparation workflows such as protein precipitation, liquid-liquid extraction, and SPE eluate collection. Manufactured from optically clear natural polystyrene (PS), the plates allow visual monitoring of well fill levels and are compatible with aqueous matrices and brief contact with common organic solvents such as acetonitrile. Supplied sterile in 5/Bag packaging, part number 222-8636-010 is well-suited to regulated bioanalytical labs, automated liquid handling platforms, and compound storage programs.

Technical Specifications

Applications

• Protein precipitation (LC-MS): The 0.5 mL well volume comfortably accommodates 100–200 µL plasma plus 200–300 µL acetonitrile or methanol precipitant; the tapered base concentrates the resulting pellet for clean aspiration after centrifugation.
• Liquid-liquid extraction (LLE): The 0.5 mL capacity is sufficient for sample plus organic extractant at standard LLE ratios, keeping solvent consumption low for high-throughput runs.
• SPE eluate collection: Aqueous and mildly organic SPE eluents are compatible with PS; the tapered bottom promotes complete recovery of small residual volumes.
• Compound library storage: DMSO-based compound stocks can be stored at –20 °C or –80 °C; the deep well format provides higher per-well volume than standard microplates.
• Automated liquid handling: The SBS-standard footprint and tapered well geometry are designed for liquid handler tip access in robotic sample prep platforms.
• Dried blood spot (DBS) elution: The deep well format readily accommodates the elution volume required for DBS punch extraction at 96-well scale.

Chemical Resistance

Quality & Procurement

Made in USA, these Caplugs® Evergreen deep well microplates are produced from FDA-compliant, BPA-free, phthalate-free, and latex-free polystyrene resin. LabSupplies.com is an authorized dealer of Caplugs® Evergreen laboratory consumables and ships from USA inventory, typically the same business day for stocking items. Tiered pricing is available for high-volume institutional, clinical, and grant-funded purchasing programs.

Frequently Asked Questions

Are these microplates sterile, and is the sterility individually documented?
Yes — the plates are supplied sterile. If your protocol requires documented sterility certification, contact our team for lot-level documentation availability before ordering.

Can these PS plates be used with acetonitrile during protein precipitation?
Brief contact with acetonitrile is acceptable for polystyrene at the volumes and contact times typical of protein precipitation workflows. However, extended immersion or high-temperature exposure can cause the PS to craze or cloud. If your method involves prolonged organic solvent exposure, consider the PPC-material sibling plate, which offers broader solvent tolerance.

What is the footprint — will these fit standard automation decks and plate sealers?
These plates conform to the SBS/ANSI standard 96-well microplate footprint, making them compatible with most automated liquid handlers, centrifuge plate buckets, and heat/adhesive plate sealers designed for standard deep well plates. Confirm Z-height requirements with your specific instrument vendor.

Is there an equivalent plate in a different material for more aggressive organic solvents?
Yes. Caplugs® Evergreen offers a 0.5 mL tapered deep well microplate in PPC (polypropylene copolymer), which provides broader resistance to organic solvents including acetonitrile, methanol, and DMSO for extended contact times. Ask our team about the PPC alternative if your workflow demands more rigorous solvent compatibility.