Caplugs® Evergreen
0.37 mL Microplates, Conical Bottom, PS, Natural — 222-8030-01F | 100/Box | Caplugs® Evergreen
0.37 mL Microplates, Conical Bottom, PS, Natural — 222-8030-01F | 100/Box | Caplugs® Evergreen
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Quick Reference
| Part Number | 222-8030-01F |
|---|---|
| Format | 96-well microplate with matching lid |
| Well Bottom | Flat |
| Well Volume | 0.37 mL max per well |
| Material | Polystyrene (PS) |
| Color | Natural |
| Pack Format | Individually Packed with lid — 100/Box |
| Sterility | Sterile |
| Primary Use | Adherent cell culture, ELISA, spectrophotometry |
| Origin | Made in USA |
| Price | $250.30/Box ($2.5030/plate) |
Technical Narrative
The Caplugs® Evergreen 222-8030-01F is a 96-Well Flat Bottom PS Sterile Microplate with Lid, 0.37 mL per well, individually packed, 100/Box. The flat-bottom 96-well plate is the universal format for adherent cell culture, ELISA, fluorescence-based assays, and spectrophotometric plate-reading — the flat well bottom provides uniform light path for absorbance measurements across all 96 wells and a flat surface for adherent cell attachment and monolayer culture. The matching lid maintains sterility during incubation, reduces evaporation, and prevents aerosol contamination in biosafety cabinet use.
PS: standard cell culture-grade polystyrene with surface treatment for enhanced cell attachment in adherent culture. Optically clear for plate reader compatibility (UV-Vis, fluorescence, luminescence). BPA-free, phthalate-free, FDA-compliant,
Procurement economics: $250.30/Box (100 plates), $2.5030/plate. Sibling: 222-8031-01V (96-Well Conical Bottom 0.30 mL/well IP). LabSupplies.com ships from USA inventory, typically same business day.
Technical Specifications
| Part Number | |
|---|---|
| Caplugs Item # | |
| Format | 96-well microplate with matching lid |
| Well Bottom | Flat |
| Well Volume | 0.37 mL max per well |
| Material | Polystyrene (PS) |
| Color | Natural |
| Lid | Included — individually packed plate + lid |
| Sterility | Sterile |
| Pack Size | 100/Box Individually Packed |
| Application | Adherent cell culture, ELISA, spectrophotometry, fluorescence assays |
| BPA / Phthalate | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
| Price | $250.30/Box ($2.5030/plate) |
96-Well Flat Bottom Microplate Applications
The is compatible with the following cell biology, assay, and diagnostic workflows.
| Application | Setting / Configuration | Notes |
|---|---|---|
| Adherent cell culture | Cell incubator, CO2, 96-well format | Flat bottom: uniform surface for monolayer culture |
| ELISA (enzyme-linked assay) | Plate reader, OD 405/450/490 nm | Flat bottom: uniform optical path for absorbance reading |
| Fluorescence plate assays | Plate reader (bottom-read), fluorescence mode | PS: low autofluorescence for fluorescence detection |
| Cell viability assays | MTT, MTS, resazurin, CellTiter-Glo | 96-well flat bottom: standard cell viability format |
| Drug dose-response | Cell biology, pharmacology screening | 96-well format: standard for 8-point dilution series |
| Luminescence assays | Luciferase reporter, ATP assay | PS flat bottom: compatible with luminescence plate readers |
| Bacterial growth assay | MIC, MBC, growth inhibition | 96-well flat: standard microbroth dilution format |
Chemical Resistance — Polystyrene (PS) — Cell Culture / Assay Microplate
Compatibility reference at typical laboratory conditions.
| Reagent / Condition | Compatibility | Notes |
|---|---|---|
| Aqueous buffers / saline | Excellent | PS inert to standard biological buffers. |
| Cell culture media (RPMI, DMEM) | Excellent | PS: standard cell culture material. |
| Formalin (10% NBF) | Excellent | Compatible for cell fixation. |
| Methanol (<100%) | Limited | Short-term compatible for fixation; prolonged contact causes crazing. |
| DMSO (<1%) | Good | Compatible at standard assay concentrations. |
| Detergents (Tween-20, Triton) | Good | Compatible at standard assay concentrations. |
| Acetone / chloroform | Not Compatible | Dissolves PS; do not use. |
| Plate reader wavelengths | Excellent | PS transparent 400–750 nm; UV (<320 nm) requires UV-transparent plates. |
Clinical & Research Laboratory Applications
Caplugs® Evergreen 96-well flat-bottom PS sterile microplates with lids serve cell biology, immunology, biochemistry, and infectious disease laboratories as the universal format for plate-based assays and adherent cell culture. The 96-well flat-bottom plate is the standard format for ELISA (enzyme-linked immunosorbent assay) — the flat PS well bottom provides a uniform optical path for plate reader absorbance measurements at the standard ELISA wavelengths (405, 450, 490 nm). Cell biology laboratories use flat-bottom 96-well plates for adherent cell culture assays — cell viability (MTT, MTS, resazurin), cytotoxicity (LDH release), proliferation (BrdU, EdU), and reporter gene assays (luciferase, GFP) — where a flat monolayer culture surface is required. The individually packed format with lid maintains sterility for each plate until the individual wrapper is opened at the BSC.
U.S. Manufacturing, Quality & Institutional Procurement
Caplugs® Evergreen products are manufactured in the United States under ISO 9001 quality management from FDA-compliant, food-contact-grade resins. BPA-free, phthalate-free, and latex-free, with material-disclosure documentation supporting CAP, CLIA, and ISO 15189 laboratory accreditation audits.
LabSupplies.com is an authorized dealer for the full Caplugs Evergreen catalog and ships directly from USA inventory — typically same business day for stocking SKUs. We offer below retail pricing on volume orders, tiered institutional and academic pricing, and direct support for grant-funded laboratories and government procurement channels. Lab managers and procurement officers can request a written quote or speak with a product specialist directly from our sales engineering team.
Frequently Asked Questions
Are the 96-well microplates sterile?
Yes. Sterile — each plate individually packed with lid, 100/Box.
What is the difference between flat-bottom and conical-bottom (222-8031-01V) 96-well plates?
Flat bottom : uniform flat PS well surface — standard for adherent cell culture, ELISA, and spectrophotometric assays where a uniform optical path and flat cell attachment surface are required. Conical bottom (222-8031-01V): V-shaped conical well bottom — concentrates cells or beads at the well tip for pelleting assays, agglutination read (bead/cell button), and any assay where maximizing recovery from the well bottom is important.
Is the 96-well flat-bottom plate compatible with standard plate readers?
Yes. The 96-well SBS/ANSI-footprint PS plate (128 mm × 86 mm) is compatible with all standard plate readers (SpectraMax, Synergy, Varioskan, CLARIOstar, etc.) that accept standard SBS-format plates. PS is transparent in the visible range (400–750 nm) for standard colorimetric and fluorescence detection. For UV absorbance (<320 nm) measurements, UV-transparent plates are required — standard PS plates are not UV-transparent.
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