7" SPE Columns with Medium Filter (45–90 µm) — Polystyrene, Natural, 200/Bag (208-3377-050)
These 7-inch polystyrene SPE columns feature an integrated medium-grade filter (45–90 µm) that balances flow rate and filtrate clarity across a wide range of sample matrices, making them the standard choice for serum, plasma, urine, environmental water, and food extracts with moderate particulate loads. Supplied non-sterile in 200/Bag format, the 208-3377-050 provides the maximum sorbent bed volume of the standard Caplugs® Evergreen column line — ideal for preparative-scale SPE, high-volume environmental sampling, and complex-matrix extraction workflows where shorter-bed columns deliver insufficient retention or wash-step separation. The integrated filter disk is molded directly into the column body, eliminating adhesive extractables at the frit interface and ensuring compatibility with sensitive downstream LC-MS and enzyme-based detection methods.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 208-3377-050 |
| Body Length | 7" |
| Filter Grade | Medium (45–90 µm) |
| Material | Polystyrene (PS) |
| Color | Natural |
| Sterility | Non-Sterile |
| Pack Size | 200/Bag |
| BPA / Phthalate | BPA-Free, Phthalate-Free |
| Country of Origin | USA |
Applications
- Preparative-scale solid-phase extraction (SPE) requiring maximum sorbent bed volume
- High-volume environmental water sampling and cleanup prior to GC-MS or LC-MS analysis
- Serum and plasma extraction for bioanalytical and clinical research workflows
- Moderately turbid urine sample processing where balanced flow and clarity are required
- Soil and sediment Soxhlet extract cleanup for complex-matrix retention
- Food and agricultural residue QC — pesticide, mycotoxin, and veterinary drug preparation
- Pharmaceutical preparative fraction collection and scale-up from smaller-format columns
- High-volume plasma and urine extract fractionation for research and bioanalytical scale-up
Compatibility
The 7-inch column body of the 208-3377-050 fits standard large-format SPE vacuum manifolds (20-port and 24-port tube-format configurations) as well as gravity-flow bench stands designed to accept a 7-inch column holder. The medium filter grade (45–90 µm) provides the balanced flow rate and filtrate clarity suited for routine manifold operation under both vacuum and gravity conditions. Laboratories scaling up methods from 5-inch columns can use the 7-inch bed directly, as the larger format accommodates 1–5 L sample loads without analyte breakthrough. For workflows requiring coarser flow (90–135 µm), refer to part 208-3376-050; for fine filtration (15–45 µm) optimized for LC-MS/MS, refer to part 208-3378-050.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline | Excellent |
| Dilute acids and bases | Good |
| Methanol (up to ~50%) | Good — within recommended concentrations |
| Methanol (>50%) / higher alcohols | Limited — not recommended for prolonged contact |
| Acetone / chloroform / aromatic solvents | Not recommended |
| Autoclaving | Not compatible — PS is not autoclavable |
Quality & Procurement
Made in USA, these Caplugs® Evergreen SPE columns are manufactured from FDA-compliant polystyrene resin under ISO 9001 quality management controls. The material is BPA-free and phthalate-free, and the adhesive-free, molded-in filter construction eliminates a common source of extractable interference in sensitive analytical workflows. LabSupplies.com is an authorized Caplugs® Evergreen dealer and ships from USA inventory, typically same business day for stocking quantities, with volume pricing available for high-throughput and institutional laboratories.
Frequently Asked Questions
What sample volumes can these columns handle?
The 7-inch bed is designed for high-volume sample loads in the 1–5 L range for environmental and preparative workflows, providing sufficient sorbent capacity to prevent analyte breakthrough under those conditions.
Why is the medium filter grade (45–90 µm) recommended for serum and plasma?
The 45–90 µm pore range delivers the optimal balance of flow rate and filtrate clarity for biological matrices with moderate particulate loads — fine enough to retain particulates that would foul downstream analytical systems, yet open enough to avoid backpressure that could lyse cells or restrict gravity flow.
Is the filter disk bonded or molded into the column?
The integrated filter disk is molded directly into the polystyrene column body rather than bonded with adhesive. This eliminates adhesive extractables at the frit interface, which is important for sensitive LC-MS and enzyme-based detection methods.
Are these columns compatible with chlorinated or aromatic solvents?
No — polystyrene is not resistant to acetone, chloroform, or aromatic solvents. These columns are suited for aqueous buffers, dilute acids and bases, and methanol concentrations up to approximately 50%. For workflows requiring aggressive organic solvents, consider a polypropylene column format.