PP Amber Extraction Columns with 45–90 µm Integrated Filter — 200/Bag (208-3358-N30)
These polypropylene amber extraction columns feature an integrated medium-porosity filter (45–90 µm) and are packaged 200 per bag for high-throughput sample preparation workflows. The amber PP construction provides integral light-protective shielding, making them especially well-suited for photosensitive analytes such as catecholamines, porphyrins, bilirubin fractions, and light-sensitive therapeutic drug metabolites — eliminating the need for foil-wrap handling under standard laboratory lighting. Supplied non-sterile, they are compatible with bench centrifuge, vacuum manifold, and gravity-flow SPE configurations.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 208-3358-N30 |
| Format | Extraction Column |
| Filter Pore Size | 45–90 µm (Medium, integrated) |
| Material | Polypropylene (PP) |
| Color | Amber (light-protective) |
| Sterility | Non-Sterile |
| Pack Size | 200/Bag |
| Centrifuge Compatibility | Up to 3000×g |
| BPA / Phthalate / Latex | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
Applications
- Solid-phase extraction (SPE) of biological fluids — serum, plasma, and urine — prior to immunoassay or photometric analysis
- Environmental sample preparation: soil and water extract cleanup where a medium particulate load is expected
- Food and agricultural extract cleanup ahead of LC-MS or GC-MS analysis, particularly for light-sensitive analytes where the amber column body eliminates a foil-wrapping step
- Extraction of catecholamines, porphyrins, bilirubin fractions, and light-sensitive therapeutic drug metabolites under standard lab lighting conditions
- Gravity-flow SPE for high-clarity aqueous matrices on a standard bench stand
Compatibility
These extraction columns are designed for use with bench centrifuge configurations using 5 mL or 15 mL collection tubes at up to 3000×g, and with vacuum SPE manifolds from Waters, Phenomenex, Supelco, and Agilent — verify your manifold's port dimensions against the column outer diameter before use. They also function in gravity-flow arrangements on a standard SPE bench stand. The integrated filter disk is molded in rather than bonded, eliminating adhesive extractables at the filter-matrix interface across all three platforms. A natural (clear) PP variant is also available for workflows where light protection is not required.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers and saline | Excellent |
| Dilute acids and bases | Excellent |
| Methanol / ethanol | Good — suitable for SPE wash and elution |
| Acetonitrile (mixed aqueous/organic) | Good — suitable for typical SPE elution systems |
| Chlorinated solvents (chloroform, DCM) | Limited — not recommended for prolonged contact |
| Aromatic solvents (toluene, xylene) | Not recommended |
| Elevated temperature / heated extraction | Good — PP is dimensionally stable under centrifuge forces |
Quality & Procurement
Made in USA, these Caplugs® Evergreen extraction columns are produced from FDA-compliant, BPA-free, phthalate-free, and latex-free polypropylene resin under ISO 9001 quality management. The molded-in filter disk avoids adhesive extractables that can compromise sensitive downstream assays. LabSupplies.com is an authorized Caplugs® Evergreen dealer shipping from USA inventory, typically the same business day for stocking items, with volume pricing available for institutional, clinical, and grant-funded laboratories purchasing ten or more bags.
Frequently Asked Questions
What filter pore size do these columns use, and what does it remove?
The integrated filter is a medium-porosity disk rated at 45–90 µm. It removes particulates in that size range from complex matrices — biological fluids, environmental extracts, and food samples — before downstream immunoassay, photometry, or chromatographic analysis.
Why are the columns amber rather than clear?
The amber PP formulation provides integral light-protective shielding for photosensitive analytes such as catecholamines, porphyrins, bilirubin fractions, and light-sensitive therapeutic drug metabolites. This eliminates the need for foil-wrapping individual columns during extraction under standard laboratory lighting.
Are the filter disks bonded or molded in?
The filter disk is molded directly into the column body — it is not bonded with an adhesive. This eliminates the risk of adhesive extractables at the filter-matrix interface, which is important for trace-level analyses and sensitive downstream assays.
Will these columns fit standard vacuum SPE manifolds?
They are compatible with vacuum manifolds from Waters, Phenomenex, Supelco, and Agilent, but manifold port dimensions vary by model. Always verify the column outer diameter against your specific manifold's port size before substituting from another supplier's columns.