Extraction Columns with Medium Filter (45–90 µm) — Polypropylene, Natural, 200/Bag (208-3355-030)
These polypropylene extraction columns feature an integrated medium-porosity filter (45–90 µm) designed for higher-volume sample cleanup of biological fluids, environmental samples, and food extracts prior to immunoassay, photometry, or chromatographic analysis. Supplied non-sterile in a convenient 200/Bag pack, part number 208-3355-030 bridges the gap between small-format mini columns and large preparative SPE tubes while remaining fully compatible with standard bench centrifuge, vacuum manifold, and gravity-flow configurations. The natural (clear) color allows visual monitoring of sample loading and flow.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 208-3355-030 |
| Format | Extraction Column |
| Integrated Filter Pore Size | 45–90 µm (Medium) |
| Material | Polypropylene (PP) |
| Color | Natural |
| Sterility | Non-Sterile |
| Pack Size | 200/Bag |
| BPA / Phthalate / Latex | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
Applications
- Solid-phase extraction (SPE) cleanup of serum, plasma, and urine prior to immunoassay or LC-MS analysis
- Environmental sample preparation — soil and water extract cleanup for moderate particulate loads in the 45–90 µm range
- Food and agricultural matrix cleanup ahead of GC-MS or LC-MS quantitation
- General biological fluid processing where higher loading capacity is required compared to mini columns
- Method development workflows requiring compatibility with a broad range of aqueous and organic modifier systems
Compatibility
These extraction columns are designed for use across three primary processing formats. In centrifuge-based workflows, they seat over standard 5 mL or 15 mL collection tubes and withstand forces up to 3,000×g, making them well suited to biological matrix extractions. For vacuum-driven SPE, they are compatible with manifold systems from Waters, Phenomenex, Supelco, and Agilent — confirm column outer diameter against your specific manifold port before use. Gravity-flow bench stands used for high-clarity aqueous matrices are also supported. A light-protected amber variant is available for workflows involving light-sensitive analytes.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline | Excellent |
| Dilute acids and bases | Excellent |
| Methanol / ethanol | Good — suitable for SPE wash and elution steps |
| Acetonitrile | Good — suitable for SPE wash and elution steps |
| Mixed organic/aqueous SPE solvents | Good — broad organic modifier range supported |
| Chlorinated solvents (e.g., chloroform, DCM) | Not recommended |
| Aromatic solvents (e.g., toluene, xylene) | Not recommended |
Quality & Procurement
Made in USA, these Caplugs® Evergreen extraction columns are manufactured from FDA-compliant, BPA-free, phthalate-free, and latex-free polypropylene resin under ISO 9001 quality management. The integrated 45–90 µm filter disk is molded in place — not adhesive-bonded — eliminating adhesive extractables at the filter-matrix interface and ensuring consistent performance across the full 200-column bag. LabSupplies.com is an authorized dealer and ships from USA inventory, typically same business day for stocking items, with volume pricing available for institutional, clinical, and grant-funded laboratories ordering at quantity.
Frequently Asked Questions
What pore size does the integrated filter provide, and is it suitable for biological fluids?
The filter provides a medium porosity of 45–90 µm, which removes particulates in that range from complex matrices. It is well suited to serum, plasma, and urine processing prior to immunoassay or chromatography, and provides adequate particulate removal for moderate-load environmental and food extract cleanup as well.
Can these columns be used with standard vacuum SPE manifolds?
Yes — they are compatible with vacuum manifold systems from Waters, Phenomenex, Supelco, and Agilent, among others. Because manifold port dimensions vary by manufacturer and model, it is good practice to verify the column outer diameter against your specific manifold before switching from your current supply.
Are these columns autoclavable or suitable for heated extraction protocols?
Polypropylene offers good dimensional stability under centrifuge forces up to 3,000×g and at elevated temperatures, making these columns suitable for heated extraction protocols. While PP is autoclavable in principle, these columns are supplied non-sterile; consult your protocol requirements before applying any sterilization step.
Is there a light-protected version for photosensitive analytes?
Yes — an amber polypropylene variant is available for workflows where analyte photodegradation is a concern, such as certain mycotoxin or vitamin assays. Ask your LabSupplies.com representative about availability and pack options for that sibling product.