3.5–5.0 mL Surety® SPE Columns with Fine Filter (15–45 µm) — LDPE, Natural, Standard 2.3″ Style (208-3045-020)
The Caplugs® Evergreen Surety® Standard column (208-3045-020) is a 3.5–5.0 mL capacity solid-phase extraction column featuring a 15–45 µm fine-filter frit, molded in natural Low-Density Polyethylene (LDPE), and supplied non-sterile in bags of 1000. The Standard 2.3″ body accommodates higher sample-load SPE workflows matched to 5 mL or 15 mL collection vessels, while the fine-filter pore size delivers high-clarity filtrate suited for LC-MS/MS injection preparation, confirmatory reference methods, dissolution testing, and pharmaceutical QC applications where residual particulates drive analytical column fouling and injection carryover.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 208-3045-020 |
| Product Type | Surety® SPE Column with Fine Filter |
| Working Capacity | 3.5–5.0 mL |
| Column Style | Standard 2.3″ |
| Filter Pore Size | 15–45 µm (Fine Filter) |
| Material | Low-Density Polyethylene (LDPE) |
| Color | Natural |
| Sterility | Non-Sterile |
| Pack Size | 1000/Bag |
| BPA / Phthalate / Latex | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
Applications
- LC-MS/MS sample preparation: filtration of protein precipitation filtrate to remove residual particulates before injection
- Confirmatory reference methods: GC-MS and LC-UV post-SPE cleanup requiring high-clarity filtrate to protect analytical columns
- Pharmaceutical QC: dissolution filtration compatible with aqueous dissolution media per USP Apparatus I/II requirements
- Bioanalytical and pharmacokinetic studies: plasma and urine extract cleanup for higher-volume sample loads
- General SPE workflows using 5 mL or 15 mL collection tube formats on vacuum manifolds or benchtop centrifuges
Compatibility
The Standard 2.3″ Surety® column is sized for 5 mL and 15 mL collection tube SPE workflows and can be operated on vacuum manifolds from Waters, Phenomenex, Supelco, and Agilent in tube-format configurations (verify tube adapter fit for your specific manifold model). It is also compatible with bench microcentrifuge protocols up to 3000×g. The defining advantage of the LDPE construction is pressure-assisted manual elution: the flexible column walls allow the analyst to apply gentle finger compression to accelerate filtrate flow without a centrifuge or vacuum manifold, making these columns highly practical for low-throughput or resource-limited settings. For labs requiring smaller working volumes, Caplugs® Evergreen offers the Surety® Mini in the 1.5–2.4 mL range (208-3040-020, 1.5″ body) and the Surety® Midi in the 2.4–3.5 mL range (208-3042-020, 1.9″ body) for complete size-matching across sample loads.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers and saline | Excellent |
| Methanol | Good — standard SPE wash/elution use |
| Acetonitrile | Good — standard SPE wash/elution use |
| Dilute acids and bases (SPE modifiers) | Good — short-term contact |
| Chlorinated solvents (e.g., chloroform, DCM) | Not recommended |
| Aromatic solvents (e.g., toluene, xylene) | Not recommended |
Quality & Procurement
Made in USA, these Surety® columns are produced from FDA-compliant, BPA-free, phthalate-free, and latex-free LDPE resin at Caplugs® Evergreen's U.S. manufacturing facility. LabSupplies.com is an authorized dealer and ships from USA inventory, typically same business day for stocking items, with volume pricing available for institutional purchasers, bioanalytical labs, and grant-funded research groups ordering multiple bags.
Frequently Asked Questions
Do these columns require a vacuum manifold or centrifuge?
No — the flexible LDPE body allows manual pressure-assisted elution by gentle finger compression, so a centrifuge or vacuum manifold is not required. They are equally compatible with vacuum manifolds and benchtop centrifuge protocols for higher-throughput workflows.
What pore size is the filter frit, and why does it matter for LC-MS/MS work?
The filter frit is 15–45 µm (fine filter). This pore range removes residual protein precipitation debris and other fine particulates from sample extracts before injection, protecting analytical columns from fouling and reducing injection carryover — a common source of variability in quantitative LC-MS/MS assays.
Are these columns sterile?
No — they are supplied non-sterile in 1000/Bag packaging, which is standard for sample preparation consumables used in analytical chemistry and SPE workflows.
How does the Standard 2.3″ Surety® column compare to the Mini and Midi sizes?
The Standard 2.3″ body (208-3045-020) handles 3.5–5.0 mL working volumes and is matched to 5 mL or 15 mL collection tubes for higher sample loads. The Surety® Mini (208-3040-020) covers 1.5–2.4 mL and the Surety® Midi (208-3042-020) covers 2.4–3.5 mL, allowing labs to select the right column size for their specific sample volume without switching suppliers or platforms.