Caplugs® Evergreen
0.37 mL Microplates, Flat Bottom, PS, Black — 290-8195-Z1F | 9/Box | Caplugs® Evergreen
0.37 mL Microplates, Flat Bottom, PS, Black — 290-8195-Z1F | 9/Box | Caplugs® Evergreen
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96-Well Flat Bottom Microplates, 0.37 mL Well Volume, PS, Black — 290-8195-Z1F | 9/Box (90/Case)
| Quick Reference — 290-8195-Z1F | |
|---|---|
| Well Format | 96-well SBS/ANSI standard footprint |
| Bottom | Flat Bottom |
| Well Volume | 0.37 mL |
| Material | Polystyrene (PS) |
| Color | Black |
| Black Plate Use | Low background fluorescence — minimizes well cross-talk |
| Primary Apps | Fluorescence intensity, FRET, HTRF, TR-FRET |
| Sterility | Non-Sterile |
| Pack Size | 9/Box (90/Case) |
| Price | $145.44 / Box ($1.6160 / plate) |
Technical Narrative
The Caplugs® Evergreen is a 96-well flat-bottom black Polystyrene (PS) microplate in SBS/ANSI standard footprint — the low-background fluorescence format for fluorescence intensity, FRET, HTRF, and TR-FRET assays where signal-to-noise ratio is the critical analytical parameter. Black PS well walls absorb stray and scattered photons that would otherwise create background signal and well-to-well cross-talk in high-sensitivity fluorescence reads — providing 5–20× lower fluorescence background versus white or natural PS plates for assays where the analyte signal is weak relative to the plate material autofluorescence. The flat bottom maintains consistent path length for quantitative fluorescence intensity reads, enzyme kinetics in fluorescence mode, and imaging-based cell assays requiring uniform focal depth.
The choice between black and white plates is determined by the detection mode. White plates maximize luminescence signal by reflecting photons toward the detector — the correct choice for luciferase reporter assays and ATP bioluminescence. Black plates minimize background by absorbing all stray light — the correct choice for fluorescence intensity, FRET, HTRF, and TR-FRET where donor bleed-through, acceptor cross-excitation, and plate material autofluorescence are the noise sources that limit assay sensitivity. Natural PS plates are the choice for absorbance-based ELISA and UV-mode assays where plate color does not affect the measurement mode.
At $145.44 per Box of 9 — $1.6160 per plate — the delivers black PS flat-bottom fluorescence assay format at bulk economics for programs running 10–100+ fluorescence plates per week. Also available: 290-8196-Z1R (96-well round-bottom PS Black, 0.32 mL, 9/Box (90/Case)).
Technical Specifications — Caplugs® Evergreen
| Specification | Value |
|---|---|
| Part Number | |
| Caplugs Item # | 2908195Z1FE1 |
| Well Format | 96-well SBS/ANSI standard footprint |
| Bottom | Flat Bottom |
| Well Volume | 0.37 mL |
| Material | Polystyrene (PS) |
| Color | Black |
| Fluorescence | Low background — black PS absorbs stray light, minimizes cross-talk |
| vs White Plate | White: maximize luminescence signal; Black: minimize fluorescence background |
| Sterility | Non-Sterile |
| Pack Size | 9/Box (90/Case) |
| Application | Fluorescence intensity, FRET, HTRF, TR-FRET, fluorescent reporter assays |
| BPA/Phthalate | BPA-free; no phthalate plasticizers |
| Country of Origin | Made in U.S.A. (California) |
Fluorescence Assay Compatibility — Flat Bottom, Black PS
Black PS plates minimize fluorescence background and well cross-talk — the standard for fluorescence-based assays where signal-to-noise ratio is the primary analytical constraint. Flat bottom ensures consistent path length for plate-reader fluorescence intensity reads:
| Application | Assay / Use | Notes |
|---|---|---|
| Fluorescence intensity (FI) | GFP, RFP, FITC, Cy3, DAPI, Hoechst stains | Black walls absorb stray light — minimize well cross-talk |
| Time-resolved fluorescence (TRF) | Europium, Terbium HTRF assays | Black plate reduces background for TRF long-lifetime signal |
| FRET assays | Förster resonance energy transfer pairs | Black minimizes donor bleed-through and acceptor cross-excitation |
| Cell-based fluorescence imaging | Fixed-cell immunofluorescence screening | Black background reduces autofluorescence from plate material |
| Fluorescent bead assays | Luminex-style multiplex bead assays | Black plate reduces background during bead fluorescence reads |
| HTRF / LanthaScreen assays | Homogeneous TR-FRET drug screening | Black required for HTRF to prevent signal interference |
| Fluorescent reporter assays | GFP/RFP reporter cell lines, live-cell imaging | Black plate suitable for both endpoint and kinetic fluorescence reads |
| Nucleic acid dye assays | PicoGreen, SYBR, EtBr well-plate quantification | Black eliminates background for sensitive nucleic acid dye reads |
Black plates minimize background — use white plates for luminescence ( is flat, 290-8196-Z1R is round). For absorbance ELISA, use natural PS flat-bottom (290-8115-01F).
Chemical Resistance — Polystyrene (PS)
Black PS resists aqueous fluorescence assay reagents. Not autoclave-compatible.
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers (PBS, TBS, HEPES) | ✓ Excellent |
| Cell culture media | ✓ Excellent |
| DMSO (≤10%) | ✓ Good |
| Short-chain alcohols (brief) | ✓ Good |
| Formalin (4%, brief fixation) | ✓ Good |
| Acetone | ✗ Avoid |
| Autoclave (121°C) | ✗ Not compatible |
Laboratory Applications
The serves cell biology programs running GFP/RFP fluorescence reporter assays; HTRF and TR-FRET drug discovery programs; FRET-based biosensor assays; nucleic acid quantification with PicoGreen and SYBR dyes; time-resolved fluorescence (TRF/LANCE) screening programs; fluorescent cell-based assays requiring low plate autofluorescence; multiplex bead fluorescence programs; and any assay requiring flat-bottom fluorescence reads with minimized background.
U.S. Manufacturing, Quality & Institutional Procurement
Every Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. Request a sample below to validate before committing to volume.
Frequently Asked Questions — Caplugs® Evergreen
When should I use black plates versus white plates for fluorescence assays?
Plate color determines how photons interact with the well. Black plates absorb stray and reflected photons — minimizing well-to-well cross-talk and plate autofluorescence background. Use black for: fluorescence intensity (FI) assays where signal-to-noise ratio is limiting, FRET/HTRF where donor bleed-through matters, any assay with weak fluorescent signals. White plates reflect photons back toward the detector — enhancing luminescence signal 2–5×. Use white for luciferase, ATP bioluminescence, and chemiluminescent ELISA. Natural plates are for absorbance-mode ELISA — plate color has minimal impact on transmission-mode reads.
Is the black plate compatible with HTRF and TR-FRET assays?
Yes. Black PS plates are the standard format for HTRF (Homogeneous Time-Resolved FRET), LANCE, and LanthaScreen TR-FRET assays. The black background eliminates the donor fluorescence leakage and acceptor pre-excitation that cause high background in HTRF reads on white or natural plates. The flat-bottom format is compatible with all standard HTRF plate readers (EnVision, PHERAstar, ClarioStar) in TR-FRET mode.
Is the compatible with automated plate readers?
Yes. The SBS/ANSI standard footprint ensures compatibility with all standard plate readers including SpectraMax, PHERAstar, Synergy (BioTek/Agilent), Infinite (Tecan), EnVision (PerkinElmer), and CLARIOstar in fluorescence intensity, HTRF, and TR-FRET modes. Flat bottom supports both top-read and bottom-read fluorescence detection modes.
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