30 mL Sterile Freestanding Centrifuge Tubes with Yellow Screw Cap & Spork — PPC, 10/Bag Unassembled (222-3534-Y80)
These 30 mL sterile polypropylene copolymer (PPC) centrifuge tubes feature a conical bottom, a freestanding natural-colored base, and a yellow screw cap — supplied unassembled with an integrated spork in 10/Bag packaging for cost-effective bench assembly. The larger 30 mL format bridges the gap above the standard 15 mL conical for high-volume cell harvest, suspension culture, and specimen processing workflows. Each bag includes a dedicated spork, eliminating the need for a separate collection instrument during post-centrifuge pellet or interface recovery.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 222-3534-Y80 |
| Capacity | 30 mL |
| Geometry | Conical bottom, freestanding base |
| Material (Tube) | Polypropylene Copolymer (PPC) |
| Tube Color | Natural |
| Cap Color | Yellow |
| Cap Style | Screw cap |
| Includes | Spork (for specimen collection) |
| Assembly State | Unassembled (tube, cap, and spork in bag) |
| Sterility | Sterile |
| Pack Size | 10/Bag |
| BPA / Phthalate / Latex | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
Applications
- Cell culture harvest: Sterile 30 mL format handles suspension culture volumes that exceed standard 15 mL conical capacity; freestanding base stands upright in a biosafety cabinet without a rack.
- T-cell and CAR-T expansion: PPC's compatibility with methanol supports downstream intracellular cytokine staining (ICS) protocols following large-scale T-cell harvest.
- Density gradient centrifugation: Natural PPC clarity allows visual monitoring of Ficoll/Percoll gradients and buffy-coat interfaces directly through the tube wall; the freestanding base keeps the tube stable post-spin.
- Cold storage: PPC's improved cold-temperature flexibility versus standard polystyrene reduces cracking risk when retrieving tubes from −20 °C or −80 °C freezers.
- SPE eluate collection (sterile): PPC's compatibility with acetonitrile and methanol makes these tubes suitable under sterile solid-phase extraction manifolds; freestanding base maintains position during elution.
- Post-centrifuge specimen collection: The included spork allows direct pellet or sediment recovery from the tube without a separate instrument, reducing per-specimen tool count.
- Stool and environmental specimen processing: The spork enables direct specimen transfer from the freestanding tube to a slide or secondary vessel, streamlining the collection workflow.
- BSC workflow assembly: The unassembled bag format allows labs to assemble tubes aseptically inside the biosafety cabinet immediately before use.
Chemical Resistance
| Reagent / Condition | Compatibility |
|---|---|
| Aqueous buffers / saline / PBS | Excellent |
| Dilute acids and bases | Excellent |
| Ethanol / isopropanol | Good — short-term contact |
| Methanol | Compatible (PPC advantage over PS) |
| Acetonitrile (ACN) | Compatible — short-term contact |
| Ficoll / Percoll density media | Excellent |
| Chlorinated solvents (chloroform, DCM) | Not recommended |
| Aromatic solvents (toluene, xylene) | Not recommended |
Quality & Procurement
Made in USA, these Caplugs® Evergreen centrifuge tubes are produced from FDA-compliant, BPA-free, phthalate-free, and latex-free PPC resin. The sterile format and freestanding design meet the demands of regulated clinical and research environments. LabSupplies.com is an authorized Caplugs® Evergreen dealer, shipping from USA inventory with same-business-day dispatch on stocking items and tiered pricing available for institutional purchasers and grant-funded labs. Companion formats include the 222-3534-G80 (green cap, 10/Bag unassembled) and 222-3533-G8D (green cap, 20/Tray assembled) for labs standardizing on a single tube platform across multiple collection workflows.
Frequently Asked Questions
Are these centrifuge tubes supplied sterile?
Yes — each tube in the 10/Bag pack is sterile and arrives unassembled, so you can open the bag and assemble tube, cap, and spork aseptically inside your biosafety cabinet immediately before use.
What is the spork, and why is it included?
The spork is a combined spoon/fork laboratory utensil packed with each tube. It allows direct post-centrifuge collection of a pellet, sediment, or interface — such as during stool or environmental specimen processing — without sourcing a separate scoop, reducing per-specimen instrument count and handling steps.
Why use PPC instead of standard polypropylene or polystyrene for these tubes?
Polypropylene copolymer (PPC) offers improved flexibility at cold temperatures, reducing the risk of cracking when tubes are retrieved from −20 °C or −80 °C storage, while also providing methanol and acetonitrile compatibility that standard polystyrene cannot match. The natural color provides optical clarity for monitoring pellets and density-gradient interfaces directly through the tube wall.
Will these tubes fit a standard bench centrifuge rotor?
The tubes are designed for use in standard rotor adapters that accommodate 30 mL conical tubes. Because rotor adapter geometry varies by manufacturer and model, verify that your specific rotor accepts the outer diameter of a 30 mL conical before use.