Caplugs® Evergreen 5" Sample Preparation Columns, Polystyrene with Coarse Filters (90-135 µm), Non-Sterile, 200/Bag
The Caplugs® Evergreen 5" Sample Preparation Columns are molded from clear polystyrene and fitted with integrated coarse frits rated at 90-135 microns. Designed for gravity-flow chromatography, resin loading, and bench-scale purification, these 5-inch columns retain particulate media while allowing free buffer flow for reproducible bind-wash-elute workflows.
Technical Specifications
| Product Type | Sample Preparation Columns (gravity-flow) |
| Volume/Size | 5" column length |
| Material | Polystyrene (PS) |
| Graduation | None |
| Filter/Frit | Coarse filter, 90-135 microns |
| Sterility | Non-Sterile |
| Pack Size | 200/Bag |
| Country of Origin | USA |
Applications
- Gravity-flow purification of proteins, peptides, and antibodies
- Packing affinity, ion-exchange, and size-exclusion resins for small-scale prep
- Solid-phase extraction and sample cleanup prior to HPLC or LC-MS
- Desalting and buffer-exchange workflows in molecular biology
- Manual screening of resin batches during method development
- Educational and teaching lab chromatography demonstrations
Quality & Procurement
labsupplies.com is an authorized Caplugs® Evergreen dealer shipping from US inventory, and these columns are Made in USA. Each bag carries a lot number to support traceability for CAP-, CLIA-, and ISO 15189-accredited laboratories. Institutional and grant-funded tiered pricing is available — contact our procurement team for volume quotes.
Frequently Asked Questions
What particle size does the coarse filter retain?
The integrated frit is rated at 90-135 microns, which retains most standard chromatography resin beads while permitting unrestricted gravity buffer flow during bind, wash, and elution steps.
Are these columns sterile?
No. These columns ship non-sterile. If your protocol requires sterility, autoclaving is not recommended for polystyrene; use a validated chemical or filtration step compatible with PS instead.
What is the column length and material?
Each column measures 5 inches in length and is molded from clear polystyrene (PS), allowing visual monitoring of resin beds and flow during sample preparation.
How are the columns packaged and sold?
They are sold in bags of 200 columns per pack. Each bag is lot-numbered for traceability and the listed price is per 200/Bag pack.