
1.5–2.0 mL Adapters, Mini Column with 45–90 µm Filter — PP, Pink, 200/Bag (208-1289-03S)
These 0.5 mL polypropylene mini columns feature an integrated 45–90 µm medium-pore filter disk and are supplied non-sterile in 200/Bag packaging for high-throughput sample preparation workflows. The pink color coding instantly distinguishes the medium-filter format on benches where multiple pore-size columns are in use, reducing cross-format errors. Part number 208-1289-03S is a reliable workhorse for serum and plasma clarification, urine particle removal, and general sample cleanup ahead of immunoassay, photometric, or analytical detection steps.
Technical Specifications
| Specification | Value |
|---|---|
| Part Number (MPN) | 208-1289-03S |
| Capacity | 0.5 mL |
| Geometry | Mini Column |
| Filter Pore Size | 45–90 µm (Medium) |
| Material | Polypropylene (PP) |
| Color | Pink |
| Sterility | Non-Sterile |
| Pack Size | 200/Bag |
| BPA / Phthalate / Latex | BPA-Free, Phthalate-Free, Latex-Free |
| Country of Origin | USA |
Applications
- Serum and plasma clarification from whole blood samples prior to immunoassay or photometric analysis
- Urine particle removal to reduce matrix interference in immunoassay drug-screening panels
- Pre-filtration of turbid sera before clinical chemistry photometric assays
- Sample cleanup ahead of LC-UV or LC-MS bioanalytical workflows, where low extractables are critical
- General particulate removal from aqueous biological matrices in research and diagnostic laboratories
Compatibility
These 0.5 mL mini columns are designed for use with 1.5–2.0 mL collection tubes on bench microcentrifuges (gravity-flow or centrifuge-assisted up to 3,000×g), and they are also compatible with 12- or 24-port vacuum SPE manifolds and 96-well SPE manifold platforms — verify column outer diameter and plate pitch against your manifold before standardizing. Manual gravity-flow on a bench stand with a 1.5 mL collection tube is suitable for high-clarity aqueous matrices. For vacuum manifold use, confirm whether a luer-tip adapter is required for your specific manifold configuration. Always verify collection tube and platform compatibility before committing to large-volume procurement.
Chemical Resistance
| Reagent / Matrix | Compatibility |
|---|---|
| Aqueous buffers / saline | Excellent |
| Dilute acids (e.g., HCl <10%) | Excellent |
| Dilute bases | Excellent |
| Ethanol (<70%) | Good — short-term contact |
| Methanol / isopropanol | Good — short-term contact |
| Acetone / chloroform / aromatic solvents | Not recommended |
| Chlorinated solvents (e.g., DCM) | Not recommended |
Quality & Procurement
Made in USA, these Caplugs® Evergreen mini columns are produced from FDA-compliant, BPA-free, phthalate-free, and latex-free polypropylene resin under ISO 9001 quality management. The 45–90 µm filter disk is integrated during molding rather than bonded post-process, eliminating adhesive leachables that could interfere with downstream enzymatic assays or mass-spectrometry detection. LabSupplies.com is an authorized Caplugs® Evergreen dealer and ships from USA inventory, typically same business day for stocking items, with volume pricing available for institutional and grant-funded laboratories procuring multiple bags.
Frequently Asked Questions
What collection tube size do these mini columns work with?
These 0.5 mL mini columns are designed for use with standard 1.5–2.0 mL microcentrifuge collection tubes in both gravity-flow and centrifuge-assisted configurations. Always confirm the column outer diameter seats properly in your specific collection tube before full deployment.
Are these columns sterile, and do they need to be for clinical sample prep?
These columns are supplied non-sterile. For most clinical chemistry and bioanalytical sample preparation workflows — serum clarification, urine filtration, and pre-LC cleanup — sterility is not required. If your downstream assay mandates a sterile fluid path, a sterile-format column would be needed instead.
Why does the filter disk pore size matter for my assay?
The 45–90 µm medium-filter pore size retains cellular debris and particulates in that size range while allowing sub-45-µm fluid and analytes to pass freely. Choosing the correct pore size prevents clogging with larger debris while ensuring the target analytes are not retained at the filter interface, which is especially important for photometric and immunoassay methods sensitive to turbidity or particulate interference.
Are there extractable or leachable concerns for LC-MS workflows?
Polypropylene is a low-extractables material at typical laboratory conditions, and the filter disk in these columns is integrated during molding rather than adhesive-bonded, removing a common source of leachables. The resin is FDA-compliant and free of BPA and phthalates, making these columns suitable for bioanalytical sample cleanup ahead of LC-UV or LC-MS detection. For novel or aggressive solvent matrices, independent verification of extractables against your specific method is always recommended.