{"product_id":"round-bottom-test-tubes-5-0ml-12-x-75mm-snap-cap-ppc-222237608ie1","title":"5.0 mL Test Tubes, Round Bottom, PPC, Natural — 222-2376-08I | 1\/Bag | Caplugs® Evergreen","description":"\u003ch2\u003e5.0 mL Round Bottom Test Tubes with Two-Position Snap Cap — PPC, Natural, Sterile, 12 × 75 mm\u003c\/h2\u003e\n\u003cp\u003eThese 5.0 mL round bottom test tubes measure 12 × 75 mm and are constructed from natural Polypropylene Copolymer (PPC) — a flexible, impact-resistant resin well suited to centrifugation and repeated freeze-thaw cycles. Each tube ships individually packed and sterile, with a two-position snap cap that supports both vented and sealed operation. Carrying part number 222-2376-08I, they are a staple format for flow cytometry, cell sorting, hematology sample preparation, and serological assays where round bottom geometry is required for efficient pellet concentration.\u003c\/p\u003e\n\n\u003ch2\u003eTechnical Specifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePart Number (MPN)\u003c\/td\u003e\n\u003ctd\u003e222-2376-08I\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume\u003c\/td\u003e\n\u003ctd\u003e5.0 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDimensions\u003c\/td\u003e\n\u003ctd\u003e12 × 75 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBottom Style\u003c\/td\u003e\n\u003ctd\u003eRound Bottom\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCap Style\u003c\/td\u003e\n\u003ctd\u003eTwo-Position Snap Cap (vented \/ sealed)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMaterial\u003c\/td\u003e\n\u003ctd\u003ePolypropylene Copolymer (PPC)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColor\u003c\/td\u003e\n\u003ctd\u003eNatural\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterility\u003c\/td\u003e\n\u003ctd\u003eSterile\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePack Size\u003c\/td\u003e\n\u003ctd\u003e1 \/ Individually Packed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBPA \/ Phthalate Free\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCountry of Origin\u003c\/td\u003e\n\u003ctd\u003eUSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow Cytometry \u0026amp; FACS:\u003c\/strong\u003e The 12 × 75 mm round bottom format is the standard geometry for flow cytometer sample probe access; natural PPC is compatible with fluorescent staining buffers and fixatives.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell Sorting \u0026amp; Immunofluorescence:\u003c\/strong\u003e Round bottom concentrates cell pellets at the tube base after centrifugation, enabling efficient resuspension and minimal sample loss during immunostaining workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHematology Sample Preparation:\u003c\/strong\u003e The 5.0 mL capacity and standard dimensions suit automated and manual hematology dilution and analysis protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSerological \u0026amp; Agglutination Assays:\u003c\/strong\u003e Round bottom geometry allows visible agglutination patterns to settle and read accurately without interference from tube corners.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecimen Collection \u0026amp; Transport:\u003c\/strong\u003e The two-position snap cap seals securely for transport and opens to a vented position for incubation, eliminating the need to swap closures between workflow steps.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFreeze-Thaw Sample Storage:\u003c\/strong\u003e PPC flexibility and impact resistance support multiple freeze-thaw cycles without cracking, making these tubes suitable for biobanking and specimen archiving at low temperatures.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eChemical Resistance\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eReagent \/ Condition\u003c\/th\u003e\n\u003cth\u003eCompatibility\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAqueous buffers \/ saline\u003c\/td\u003e\n\u003ctd\u003eExcellent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDilute acids\u003c\/td\u003e\n\u003ctd\u003eExcellent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDilute bases\u003c\/td\u003e\n\u003ctd\u003eExcellent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMethanol\u003c\/td\u003e\n\u003ctd\u003eCompatible\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIsopropanol\u003c\/td\u003e\n\u003ctd\u003eCompatible\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcetonitrile\u003c\/td\u003e\n\u003ctd\u003eCompatible\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAromatic solvents (e.g., toluene, xylene)\u003c\/td\u003e\n\u003ctd\u003eLimited — not recommended for prolonged contact\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003ch2\u003eQuality \u0026amp; Procurement\u003c\/h2\u003e\n\u003cp\u003eMade in USA at Caplugs® Evergreen ISO-certified facilities, these tubes are manufactured from virgin-grade, FDA-registered PPC resin that is BPA-free and phthalate-free. Lot-level quality controls ensure consistent tube geometry, wall thickness, and dimensional uniformity across every individually packed unit. LabSupplies.com is an authorized Caplugs® Evergreen dealer shipping from USA inventory, with volume and institutional pricing available for clinical labs, research institutions, and biotech procurement programs operating under CAP, CLIA, or ISO 15189 frameworks.\u003c\/p\u003e\n\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAre these tubes supplied sterile, and is sterility maintained in individual packaging?\u003c\/strong\u003e\u003cbr\u003eYes — each tube is individually packed and sterile. The sealed packaging preserves sterility from the point of manufacture through point-of-use opening, making these tubes ready for cell culture, specimen collection, and other contamination-sensitive workflows without additional sterilization steps.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eWhat does the two-position snap cap do, and can I switch between positions mid-protocol?\u003c\/strong\u003e\u003cbr\u003eThe two-position snap cap offers a vented configuration for gas exchange during cell incubation and a fully sealed configuration for containment during centrifugation or transport. The positions can be toggled by hand mid-protocol without tools, eliminating the need to swap or store separate closures.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eWhy is a round bottom preferred over a conical bottom for flow cytometry?\u003c\/strong\u003e\u003cbr\u003eRound bottom tubes allow the flow cytometer's sample probe to aspirate from the very base of the tube without the restriction that a conical geometry can create, and the curved interior wall helps cell pellets resuspend evenly with minimal vortexing. The 12 × 75 mm dimension is the widely adopted standard format for most flow cytometry instrument carousels.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eIs PPC autoclavable, and can these tubes be reused after sterilization?\u003c\/strong\u003e\u003cbr\u003ePolypropylene Copolymer is generally autoclavable; however, because these tubes are individually packed and supplied sterile for single-use workflows, Caplugs® Evergreen does not validate or recommend reuse after autoclaving. 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