{"product_id":"caplugs-290-8229-z1l-384-well-microplate-lid-ps-black","title":"8218–01 L Microplates, PS, Black — 290-8229-Z1L | 15\/Box | Caplugs® Evergreen","description":"\u003ch2\u003e384-Well Microplate Lids, PS, Black — 290-8229-Z1L | 15\/Box (105\/Case)\u003c\/h2\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e\u003cth colspan=\"2\"\u003eQuick Reference — 290-8229-Z1L\u003c\/th\u003e\u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eWells\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e384-well SBS format\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eColor\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eBlack\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eLight Block\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e✓ Black lid blocks ambient light from plate\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eUse With\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eBlack 384-well plates (290-8225-Z10) for light-sensitive assays\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eAutoclave\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e✓ Compatible (121°C)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e15\/Box (105\/Case)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePrice\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e$77.67 \/ Box ($0.7398 \/ lid)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eTechnical Narrative\u003c\/h2\u003e \u003cp\u003eThe Caplugs® Evergreen is a black PS 384-well microplate lid — the light-excluding evaporation control solution for 384-well fluorescence HTS and HTRF assay incubation. Unlike natural PS lids that allow ambient light to enter the plate during incubation, the black PS construction blocks light from reaching well contents — preventing the photodegradation of light-sensitive fluorescent reagents (fluorescent antibodies, HTRF acceptor dyes, fluorescent probes) that occurs with transparent lids during incubation steps under laboratory fluorescent or LED lighting.\u003c\/p\u003e \u003cp\u003eFor HTRF assays in particular, ambient light exposure during the compound-plus-antibody incubation step can quench the europium cryptate donor fluorescence that drives the time-resolved FRET signal. A black lid over a black plate provides complete light exclusion throughout the incubation period without requiring incubator light cutoff or foil plate wrapping. PS construction is autoclave-compatible for sterilization before cell-based 384-well assays requiring sterile plate coverage.\u003c\/p\u003e \u003cp\u003eAt $77.67 per Box of 15 — $0.7398 per lid — the delivers light-excluding 384-well coverage at per-lid economics for high-volume HTRF and fluorescence HTS programs. Pairs with the 290-8225-Z10 black 384-well plate for complete light exclusion from both plate and lid.\u003c\/p\u003e \u003ch2\u003eTechnical Specifications — Caplugs® Evergreen \u003c\/h2\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eSpecification\u003c\/th\u003e \u003cth\u003eValue\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003ePart Number\u003c\/td\u003e \u003ctd\u003e\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCaplugs Item #\u003c\/td\u003e \u003ctd\u003e2908229Z1LE1\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eWell Format\u003c\/td\u003e \u003ctd\u003e384-well SBS\/ANSI standard footprint\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eMaterial\u003c\/td\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eColor\u003c\/td\u003e \u003ctd\u003eBlack\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eLight Block\u003c\/td\u003e \u003ctd\u003eBlack lid prevents ambient light from entering well during incubation\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAutoclave\u003c\/td\u003e \u003ctd\u003e✓ Compatible (121°C \/ 15 psi)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eSterility\u003c\/td\u003e \u003ctd\u003eNon-Sterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003ePack Size\u003c\/td\u003e \u003ctd\u003e15\/Box (105\/Case)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eApplication\u003c\/td\u003e \u003ctd\u003e384-well fluorescence HTS, HTRF, light-sensitive incubation\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eBPA\/Phthalate\u003c\/td\u003e \u003ctd\u003eBPA-free; no phthalate plasticizers\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCountry of Origin\u003c\/td\u003e \u003ctd\u003eMade in U.S.A. (California)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eBlack 384-Well Lid Workflow Compatibility — \u003c\/h2\u003e \u003cp\u003eThe black PS lid combines evaporation control with ambient light exclusion for light-sensitive 384-well assay incubation:\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eApplication\u003c\/th\u003e \u003cth\u003eAssay \/ Use\u003c\/th\u003e \u003cth\u003eNotes\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eHTRF \/ TR-FRET incubation\u003c\/td\u003e \u003ctd\u003e384-well HTRF assay compound incubation\u003c\/td\u003e \u003ctd\u003eBlack lid blocks ambient light that can quench TR-FRET fluorophores\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFluorescent reagent incubation\u003c\/td\u003e \u003ctd\u003eLight-sensitive primary antibody, dye incubation\u003c\/td\u003e \u003ctd\u003eBlack lid prevents photodegradation of fluorescent reagents\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e384-well cell culture\u003c\/td\u003e \u003ctd\u003eCell-based assay incubation in CO2 incubator\u003c\/td\u003e \u003ctd\u003eBlack lid reduces evaporation while blocking incubator light exposure\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCompound plate storage\u003c\/td\u003e \u003ctd\u003e384-well compound plates in dark storage\u003c\/td\u003e \u003ctd\u003eBlack lid maintains dark conditions during compound plate storage\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFluorescent probe protection\u003c\/td\u003e \u003ctd\u003eDAPI, calcein, Hoechst, Alamar Blue protection\u003c\/td\u003e \u003ctd\u003eBlack lid prevents photobleaching during incubation period\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eOvernight plate incubation\u003c\/td\u003e \u003ctd\u003eExtended incubation under light exclusion\u003c\/td\u003e \u003ctd\u003eBlack lid provides light exclusion + evaporation control\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003cp\u003e\u003cem\u003eBlack lid pairs specifically with black 384-well plates (290-8225-Z10) for maximum light exclusion in fluorescence HTS. For transparent monitoring of well contents during incubation, use natural PS lid (290-8218-01L) instead.\u003c\/em\u003e\u003c\/p\u003e \u003ch2\u003eChemical Resistance — Polystyrene (PS)\u003c\/h2\u003e \u003cp\u003ePS resists standard aqueous assay reagents. Autoclave-compatible.\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eReagent \/ Condition\u003c\/th\u003e \u003cth\u003eCompatibility\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eAqueous buffers\u003c\/td\u003e \u003ctd\u003e✓ Excellent\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCell culture media\u003c\/td\u003e \u003ctd\u003e✓ Excellent\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDMSO (≤10%)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eShort-chain alcohols (brief)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAcetone\u003c\/td\u003e \u003ctd\u003e✗ Avoid\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAutoclave\u003c\/td\u003e \u003ctd\u003e✗ Not compatible\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eLaboratory Applications\u003c\/h2\u003e \u003cp\u003eThe serves HTRF and TR-FRET HTS programs requiring light exclusion during fluorophore incubation; fluorescence intensity HTS programs protecting light-sensitive antibody and dye incubation steps; 384-well cell-based assay programs using calcein, Alamar Blue, or DAPI under CO2 incubator light exposure; and any 384-well workflow requiring combined evaporation control and ambient light exclusion during incubation.\u003c\/p\u003e \u003ch2\u003eU.S. Manufacturing, Quality \u0026amp; Institutional Procurement\u003c\/h2\u003e \u003cp\u003eEvery Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. \u003ca href=\"#request-sample\"\u003eRequest a sample below\u003c\/a\u003e to validate before committing to volume.\u003c\/p\u003e \u003ch2\u003eFrequently Asked Questions — Caplugs® Evergreen \u003c\/h2\u003e \u003ch3\u003eWhy use a black lid instead of a natural PS lid for 384-well fluorescence assays?\u003c\/h3\u003e \u003cp\u003eNatural PS lids are transparent — ambient laboratory light passes through them and into the plate wells during incubation. For light-sensitive fluorescent reagents (HTRF donor dyes, fluorescent antibodies, DAPI, calcein, Hoechst), this ambient light exposure can cause photobleaching and quenching during incubation, reducing signal and assay reproducibility. The black lid blocks ambient light from reaching well contents throughout the incubation period — the same reason black plates are used instead of natural plates for fluorescence assays.\u003c\/p\u003e \u003ch3\u003eIs the black lid autoclave-compatible for cell-based 384-well assays?\u003c\/h3\u003e \u003cp\u003eYes. The is molded from PS and is autoclave-compatible at 121°C \/ 15 psi for sterilization before use in cell-based 384-well assays. Autoclave in a sealed pouch — allow to cool before placing on culture plates.\u003c\/p\u003e \u003ch3\u003eShould I use the black lid with the black plate, or can I use it with any 384-well plate?\u003c\/h3\u003e \u003cp\u003eThe black lid fits any standard SBS 384-well plate — you can use it with black (290-8225-Z10), white (290-8221-W10), or natural (290-8217-010) 384-well plates. The light-blocking benefit is most relevant when used with black plates for complete light exclusion from all directions. Using a black lid on a white or natural plate still blocks light from above but does not prevent side-wall light entry in the plate body.\u003c\/p\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"384-Well Microplate Lids, Non-Sterile, Polystyrene (PS), Black \\u2014 290-8229-Z1L | 15\/Box (105\/Case) | Caplugs\\u00ae Evergreen\",\n  \"description\": \"Caplugs\\u00ae Evergreen 290-8229-Z1L. 384-well PS black microplate lids, 15\/Box (105\/Case). Low-background fluorescence. BPA-free. USA.\",\n  \"sku\": \"2908229Z1LE1\",\n  \"mpn\": \"290-8229-Z1L\",\n  \"material\": \"Polystyrene (PS)\",\n  \"color\": \"Black\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"Caplugs\\u00ae Evergreen\"\n  },\n  \"manufacturer\": {\n    \"@type\": \"Organization\",\n    \"name\": \"Caplugs\\u00ae Evergreen\",\n    \"address\": {\n      \"@type\": \"PostalAddress\",\n      \"addressCountry\": \"US\",\n      \"addressRegion\": \"CA\"\n    }\n  },\n  \"offers\": {\n    \"@type\": \"Offer\",\n    \"price\": \"77.67\",\n    \"priceCurrency\": \"USD\",\n    \"availability\": \"https:\/\/schema.org\/InStock\",\n    \"itemCondition\": \"https:\/\/schema.org\/NewCondition\",\n    \"seller\": {\n      \"@type\": \"Organization\",\n      \"name\": \"LabSupplies\"\n    }\n  },\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Pack Size\",\n      \"value\": \"105 \/ Box\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Sterility\",\n      \"value\": \"Non-Sterile\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Country of Origin\",\n      \"value\": \"United States\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"BPA Free\",\n      \"value\": \"Yes\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Well Format\",\n      \"value\": \"384-well SBS\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Well Volume\",\n      \"value\": \"N\/A\"\n    }\n  ]\n}\n\u003c\/script\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Why use a black lid instead of a natural PS lid for 384-well fluorescence assays?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Natural PS lids are transparent \\u2014 ambient laboratory light passes through them and into the plate wells during incubation. For light-sensitive fluorescent reagents (HTRF donor dyes, fluorescent antibodies, DAPI, calcein, Hoechst), this ambient light exposure can cause photobleaching and quenching during incubation, reducing signal and assay reproducibility. The black lid blocks ambient light from reaching well contents throughout the incubation period \\u2014 the same reason black plates are used instead of natural plates for fluorescence assays.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Is the black lid autoclave-compatible for cell-based 384-well assays?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes. The 290-8229-Z1L is molded from PS and is autoclave-compatible at 121\\u00b0C \/ 15 psi for sterilization before use in cell-based 384-well assays. Autoclave in a sealed pouch \\u2014 allow to cool before placing on culture plates.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Should I use the black lid with the black plate, or can I use it with any 384-well plate?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The 290-8229-Z1L black lid fits any standard SBS 384-well plate \\u2014 you can use it with black (290-8225-Z10), white (290-8221-W10), or natural (290-8217-010) 384-well plates. The light-blocking benefit is most relevant when used with black plates for complete light exclusion from all directions. 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