{"product_id":"caplugs-290-8196-z1r-96-well-round-bottom-microplate-ps-black","title":"0.32 mL Microplates, Round Bottom, PS, Black, BD® Compatible — 290-8196-Z1R | 9\/Box | Caplugs® Evergreen","description":"\u003ch2\u003e96-Well Round Bottom Microplates, 0.32 mL Well Volume, PS, Black — 290-8196-Z1R | 9\/Box (90\/Case)\u003c\/h2\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e\u003cth colspan=\"2\"\u003eQuick Reference — 290-8196-Z1R\u003c\/th\u003e\u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eWell Format\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e96-well SBS\/ANSI standard footprint\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eBottom\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eRound Bottom\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eWell Volume\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e0.32 mL\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eColor\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eBlack\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eBlack Plate Use\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eLow background fluorescence — minimizes well cross-talk\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePrimary Apps\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eFluorescence intensity, FRET, HTRF, TR-FRET\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eSterility\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eNon-Sterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e9\/Box (90\/Case)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePrice\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e$145.44 \/ Box ($1.6160 \/ plate)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eTechnical Narrative\u003c\/h2\u003e \u003cp\u003eThe Caplugs® Evergreen is a 96-well round-bottom black Polystyrene (PS) microplate in SBS\/ANSI standard footprint — the low-background fluorescence format for fluorescence intensity, FRET, HTRF, and TR-FRET assays where signal-to-noise ratio is the critical analytical parameter. Black PS well walls absorb stray and scattered photons that would otherwise create background signal and well-to-well cross-talk in high-sensitivity fluorescence reads — providing 5–20× lower fluorescence background versus white or natural PS plates for assays where the analyte signal is weak relative to the plate material autofluorescence. The round (U-bottom) geometry allows suspension cells and fluorescent beads to settle uniformly by gravity — enabling efficient suspension cell fluorescence reads with minimal spatial variability from non-uniform cell distribution.\u003c\/p\u003e \u003cp\u003eThe choice between black and white plates is determined by the detection mode. White plates maximize luminescence signal by reflecting photons toward the detector — the correct choice for luciferase reporter assays and ATP bioluminescence. Black plates minimize background by absorbing all stray light — the correct choice for fluorescence intensity, FRET, HTRF, and TR-FRET where donor bleed-through, acceptor cross-excitation, and plate material autofluorescence are the noise sources that limit assay sensitivity. Natural PS plates are the choice for absorbance-based ELISA and UV-mode assays where plate color does not affect the measurement mode.\u003c\/p\u003e \u003cp\u003eAt $145.44 per Box of 9 — $1.6160 per plate — the delivers black PS round-bottom fluorescence assay format at bulk economics for programs running 10–100+ fluorescence plates per week. Also available: 290-8195-Z1F (96-well flat-bottom PS Black, 0.37 mL, 9\/Box (90\/Case)).\u003c\/p\u003e \u003ch2\u003eTechnical Specifications — Caplugs® Evergreen \u003c\/h2\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eSpecification\u003c\/th\u003e \u003cth\u003eValue\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003ePart Number\u003c\/td\u003e \u003ctd\u003e\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCaplugs Item #\u003c\/td\u003e \u003ctd\u003e2908196Z1RE1\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eWell Format\u003c\/td\u003e \u003ctd\u003e96-well SBS\/ANSI standard footprint\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eBottom\u003c\/td\u003e \u003ctd\u003eRound Bottom\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eWell Volume\u003c\/td\u003e \u003ctd\u003e0.32 mL\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eMaterial\u003c\/td\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eColor\u003c\/td\u003e \u003ctd\u003eBlack\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFluorescence\u003c\/td\u003e \u003ctd\u003eLow background — black PS absorbs stray light, minimizes cross-talk\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003evs White Plate\u003c\/td\u003e \u003ctd\u003eWhite: maximize luminescence signal; Black: minimize fluorescence background\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eSterility\u003c\/td\u003e \u003ctd\u003eNon-Sterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003ePack Size\u003c\/td\u003e \u003ctd\u003e9\/Box (90\/Case)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eApplication\u003c\/td\u003e \u003ctd\u003eFluorescence intensity, FRET, HTRF, TR-FRET, fluorescent reporter assays\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eBPA\/Phthalate\u003c\/td\u003e \u003ctd\u003eBPA-free; no phthalate plasticizers\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCountry of Origin\u003c\/td\u003e \u003ctd\u003eMade in U.S.A. (California)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eFluorescence Assay Compatibility — Round Bottom, Black PS\u003c\/h2\u003e \u003cp\u003eBlack PS plates minimize fluorescence background and well cross-talk — the standard for fluorescence-based assays where signal-to-noise ratio is the primary analytical constraint. Round bottom for suspension cell fluorescence assays where cells must settle uniformly:\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eApplication\u003c\/th\u003e \u003cth\u003eAssay \/ Use\u003c\/th\u003e \u003cth\u003eNotes\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eSuspension cell fluorescence\u003c\/td\u003e \u003ctd\u003eGFP\/RFP in Jurkat, lymphocyte, PBMC\u003c\/td\u003e \u003ctd\u003eRound bottom for suspension cells + black for low-background FI\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eHAI with fluorescent read\u003c\/td\u003e \u003ctd\u003eFluorescence-detected hemagglutination\u003c\/td\u003e \u003ctd\u003eBlack round bottom for combined agglutination + fluorescence\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFluorescent bead (suspension)\u003c\/td\u003e \u003ctd\u003eCytokine bead array with FI detection\u003c\/td\u003e \u003ctd\u003eBlack reduces background during bead fluorescence reads\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFRET in suspension cells\u003c\/td\u003e \u003ctd\u003eDonor\/acceptor FRET in suspension cell lines\u003c\/td\u003e \u003ctd\u003eBlack round bottom minimizes cross-talk for suspension cell FRET\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCell viability (live\/dead)\u003c\/td\u003e \u003ctd\u003eCalcein AM, PI, Annexin V in suspension\u003c\/td\u003e \u003ctd\u003eRound bottom pellets suspension cells; black minimizes background\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eTime-resolved FI (suspension)\u003c\/td\u003e \u003ctd\u003eTRF assays with suspension cells\u003c\/td\u003e \u003ctd\u003eBlack round bottom for TRF in non-adherent cell formats\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAgglutination + fluorescence\u003c\/td\u003e \u003ctd\u003eRBC-based assay with fluorescent antibody label\u003c\/td\u003e \u003ctd\u003eBlack round bottom for combined agglutination-fluorescence protocol\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eMultiplex bead (pellet-based)\u003c\/td\u003e \u003ctd\u003eMagnetic bead suspension fluorescence assay\u003c\/td\u003e \u003ctd\u003eRound bottom for bead pelleting; black for low-background FI\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003cp\u003e\u003cem\u003eBlack plates minimize background — use white plates when luminescence signal enhancement is needed alongside suspension culture. For HAI agglutination assays requiring visual scoring, natural PS round-bottom (290-8117-01R) is preferred.\u003c\/em\u003e\u003c\/p\u003e \u003ch2\u003eChemical Resistance — Polystyrene (PS)\u003c\/h2\u003e \u003cp\u003eBlack PS resists aqueous fluorescence assay reagents. Not autoclave-compatible.\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eReagent \/ Condition\u003c\/th\u003e \u003cth\u003eCompatibility\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eAqueous buffers (PBS, TBS, HEPES)\u003c\/td\u003e \u003ctd\u003e✓ Excellent\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCell culture media\u003c\/td\u003e \u003ctd\u003e✓ Excellent\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDMSO (≤10%)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eShort-chain alcohols (brief)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFormalin (4%, brief fixation)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAcetone\u003c\/td\u003e \u003ctd\u003e✗ Avoid\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAutoclave (121°C)\u003c\/td\u003e \u003ctd\u003e✗ Not compatible\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eLaboratory Applications\u003c\/h2\u003e \u003cp\u003eThe serves cell biology programs running fluorescence assays in suspension cells (GFP\/RFP in Jurkat, lymphocytes, PBMC); FRET-based suspension cell assays; cytokine bead array and multiplex bead programs with FI detection; HAI\/agglutination assays combined with fluorescent antibody detection; live\/dead fluorescence viability in suspension; and any assay requiring low-background fluorescence reads with suspension cell pelleting behavior.\u003c\/p\u003e \u003ch2\u003eU.S. Manufacturing, Quality \u0026amp; Institutional Procurement\u003c\/h2\u003e \u003cp\u003eEvery Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. \u003ca href=\"#request-sample\"\u003eRequest a sample below\u003c\/a\u003e to validate before committing to volume.\u003c\/p\u003e \u003ch2\u003eFrequently Asked Questions — Caplugs® Evergreen \u003c\/h2\u003e \u003ch3\u003eWhen should I use black round-bottom versus black flat-bottom plates for fluorescence?\u003c\/h3\u003e \u003cp\u003eBoth provide low-background fluorescence. The difference is the well geometry. Black flat-bottom (290-8195-Z1F) is for adherent cells and assays requiring consistent path length for quantitative plate-reader FI reads. Black round-bottom  is for suspension cells and beads that need to settle uniformly by gravity — providing consistent cell distribution for suspension cell fluorescence, bead-based multiplexing, and agglutination assays combined with fluorescent detection. Choose based on whether your cell or particle is adherent (flat) or suspension (round).\u003c\/p\u003e \u003ch3\u003eIs the black round-bottom plate suitable for cytokine bead array (CBA) assays?\u003c\/h3\u003e \u003cp\u003eYes. Black round-bottom plates are well-suited for cytokine bead array (CBA, LEGENDplex, BD CBA) and Luminex-style multiplex bead assays. The round bottom pellets fluorescent beads uniformly during centrifugation and allows efficient supernatant removal, while the black walls minimize background fluorescence during the fluorescence intensity read. Confirm compatibility with your specific CBA workflow — some protocols specify flat-bottom for non-centrifugation bead read formats.\u003c\/p\u003e \u003ch3\u003eIs the compatible with standard fluorescence plate readers?\u003c\/h3\u003e \u003cp\u003eYes. The SBS\/ANSI standard footprint ensures compatibility with all standard plate readers in fluorescence intensity mode. For suspension cell fluorescence with round-bottom plates, top-read mode is standard — the reader measures fluorescence from above the settled cell layer. Bottom-read fluorescence is also supported but path length variability from round-bottom geometry should be considered for quantitative assays.\u003c\/p\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"96-Well Microplates, Round Bottom, 0.32 mL Well Volume, Non-Sterile, Polystyrene (PS), Black \\u2014 290-8196-Z1R | 9\/Box (90\/Case) | Caplugs\\u00ae Evergreen\",\n  \"description\": \"Caplugs\\u00ae Evergreen 290-8196-Z1R. 96-well round-bottom PS black microplate, 0.32 mL, 9\/Box (90\/Case). Fluorescence, FRET, HTRF. BPA-free. USA.\",\n  \"sku\": \"2908196Z1RE1\",\n  \"mpn\": \"290-8196-Z1R\",\n  \"material\": \"Polystyrene (PS)\",\n  \"color\": \"Black\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"Caplugs\\u00ae Evergreen\"\n  },\n  \"manufacturer\": {\n    \"@type\": \"Organization\",\n    \"name\": \"Caplugs\\u00ae Evergreen\",\n    \"address\": {\n      \"@type\": \"PostalAddress\",\n      \"addressCountry\": \"US\",\n      \"addressRegion\": \"CA\"\n    }\n  },\n  \"offers\": {\n    \"@type\": \"Offer\",\n    \"price\": \"145.44\",\n    \"priceCurrency\": \"USD\",\n    \"availability\": \"https:\/\/schema.org\/InStock\",\n    \"itemCondition\": \"https:\/\/schema.org\/NewCondition\",\n    \"seller\": {\n      \"@type\": \"Organization\",\n      \"name\": \"LabSupplies\"\n    }\n  },\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Pack Size\",\n      \"value\": \"90 \/ Box\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Sterility\",\n      \"value\": \"Non-Sterile\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Country of Origin\",\n      \"value\": \"United States\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"BPA Free\",\n      \"value\": \"Yes\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Well Format\",\n      \"value\": \"96-well SBS\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Well Volume\",\n      \"value\": \"0.32 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Bottom Geometry\",\n      \"value\": \"Round\"\n    }\n  ]\n}\n\u003c\/script\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"When should I use black round-bottom versus black flat-bottom plates for fluorescence?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Both provide low-background fluorescence. The difference is the well geometry. Black flat-bottom (290-8195-Z1F) is for adherent cells and assays requiring consistent path length for quantitative plate-reader FI reads. Black round-bottom (290-8196-Z1R) is for suspension cells and beads that need to settle uniformly by gravity \\u2014 providing consistent cell distribution for suspension cell fluorescence, bead-based multiplexing, and agglutination assays combined with fluorescent detection. Choose based on whether your cell or particle is adherent (flat) or suspension (round).\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Is the black round-bottom plate suitable for cytokine bead array (CBA) assays?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes. Black round-bottom plates are well-suited for cytokine bead array (CBA, LEGENDplex, BD CBA) and Luminex-style multiplex bead assays. The round bottom pellets fluorescent beads uniformly during centrifugation and allows efficient supernatant removal, while the black walls minimize background fluorescence during the fluorescence intensity read. Confirm compatibility with your specific CBA workflow \\u2014 some protocols specify flat-bottom for non-centrifugation bead read formats.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Is the 290-8196-Z1R compatible with standard fluorescence plate readers?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes. The SBS\/ANSI standard footprint ensures compatibility with all standard plate readers in fluorescence intensity mode. For suspension cell fluorescence with round-bottom plates, top-read mode is standard \\u2014 the reader measures fluorescence from above the settled cell layer. 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