{"product_id":"caplugs-290-8117-01r-96-well-roundubottom-microplate-ps-natural","title":"0.32 mL Microplates, Round Bottom, PS, Natural — 290-8117-01R | 10\/Box | Caplugs® Evergreen","description":"\u003ch2\u003e96-Well Round (U-Bottom) Bottom Microplates, 0.32 mL Well Volume, PS, Natural — 290-8117-01R | 10\/Box (100\/Case)\u003c\/h2\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e\u003cth colspan=\"2\"\u003eQuick Reference — 290-8117-01R\u003c\/th\u003e\u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eWell Format\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e96-well SBS\/ANSI standard footprint\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eBottom Geometry\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eRound (U-Bottom) Bottom\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eWell Volume\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e0.32 mL\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eColor\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eNatural\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePrimary Use\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eSuspension cell culture\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eSterility\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eNon-Sterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e10\/Box (100\/Case)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePrice\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e$120.33 \/ Box ($1.2033 \/ plate)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eTechnical Narrative\u003c\/h2\u003e \u003cp\u003eThe Caplugs® Evergreen is a 96-well round (u-bottom) bottom Polystyrene (PS) microplate in natural for suspension cell culture, cell-based aggregation assays, and wash-based immunoassays requiring cell pelleting by gravity. The U-bottom geometry allows cells and particles to settle by gravity into a uniform central pellet — enabling consistent supernatant aspiration and re-suspension without the cell loss that occurs with flat or V-bottom wells during aspiration. Natural (clear) PS for suspension cell culture and visual agglutination reads. The U-bottom geometry is the defining feature — color choice is secondary to bottom geometry for these applications.\u003c\/p\u003e \u003cp\u003eAll 96-well Caplugs® Evergreen microplates conform to the SBS\/ANSI microplate standard footprint (127.76 × 85.47 mm), ensuring compatibility with all standard plate readers (SpectraMax, PHERAstar, Synergy, Infinite, EnVision), automated liquid handlers (Hamilton, Tecan, Bravo), plate washers, and robotic gripper arm systems. The 0.32 mL well volume accommodates standard assay reagent addition sequences across the primary applications for round (u-bottom) format plates. PS construction is compatible with all standard aqueous assay buffers, culture media, and detection reagents.\u003c\/p\u003e \u003cp\u003eAt $120.33 per Box of 10 — $1.2033 per plate — the delivers round (u-bottom) PS microplate economics for programs running 10–200+ plates per week. The 10\/Box (100\/Case) format supports both per-box and case-quantity ordering.\u003c\/p\u003e \u003ch2\u003eTechnical Specifications — Caplugs® Evergreen \u003c\/h2\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eSpecification\u003c\/th\u003e \u003cth\u003eValue\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003ePart Number\u003c\/td\u003e \u003ctd\u003e\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCaplugs Item #\u003c\/td\u003e \u003ctd\u003e290811701RE1\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eWell Format\u003c\/td\u003e \u003ctd\u003e96-well SBS\/ANSI standard footprint\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eBottom Geometry\u003c\/td\u003e \u003ctd\u003eRound (U-Bottom) Bottom\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eWell Volume\u003c\/td\u003e \u003ctd\u003e0.32 mL (working volume typically 50–80% of max)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eMaterial\u003c\/td\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eColor\u003c\/td\u003e \u003ctd\u003eNatural\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eSterility\u003c\/td\u003e \u003ctd\u003eNon-Sterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003ePack Size\u003c\/td\u003e \u003ctd\u003e10\/Box (100\/Case)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eApplication\u003c\/td\u003e \u003ctd\u003eSuspension cell culture\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eBPA \/ Phthalate\u003c\/td\u003e \u003ctd\u003eBPA-free; no phthalate plasticizers\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCountry of Origin\u003c\/td\u003e \u003ctd\u003eMade in U.S.A. (California)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eAssay \u0026amp; Application Compatibility — Round (U-Bottom) Bottom, Natural\u003c\/h2\u003e \u003cp\u003eRound (U-Bottom) bottom PS natural plates are the standard format for suspension cell culture, cell-based aggregation assays, and wash-based immunoassays requiring cell pelleting by gravity. Compatible workflows:\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eApplication\u003c\/th\u003e \u003cth\u003eAssay \/ Use\u003c\/th\u003e \u003cth\u003eNotes\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eSuspension cell culture\u003c\/td\u003e \u003ctd\u003eLymphocytes, PBMCs, hybridomas, bacteria\u003c\/td\u003e \u003ctd\u003eRound bottom allows cells to settle uniformly into single-cell pellet\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003ePBMC isolation and culture\u003c\/td\u003e \u003ctd\u003ePrimary blood mononuclear cell assays\u003c\/td\u003e \u003ctd\u003eU-bottom collects cells at well center for efficient aspirate removal\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAgglutination assays (HAI, HI)\u003c\/td\u003e \u003ctd\u003eHemagglutination inhibition, viral titer\u003c\/td\u003e \u003ctd\u003eRound bottom distributes RBC evenly — standard HAI format plate\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eHybridoma screening\u003c\/td\u003e \u003ctd\u003eAntibody-producing clone culture\u003c\/td\u003e \u003ctd\u003eRound bottom supports hybridoma growth and supernatant harvest\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eImmunoprecipitation prep\u003c\/td\u003e \u003ctd\u003eAntibody-bead complex pelleting\u003c\/td\u003e \u003ctd\u003eRound bottom enables complete bead pelleting at low g-force\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eBacterial culture \/ MIC assay\u003c\/td\u003e \u003ctd\u003eMinimum inhibitory concentration broth dilution\u003c\/td\u003e \u003ctd\u003eRound bottom shows clear button vs turbidity distinction for MIC reads\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCytokine bead array (CBA)\u003c\/td\u003e \u003ctd\u003eMultiplex bead-based cytokine assay\u003c\/td\u003e \u003ctd\u003eU-bottom for bead pelleting during wash steps\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCell washing \/ serial dilution\u003c\/td\u003e \u003ctd\u003eMulti-step wash and dilution protocols\u003c\/td\u003e \u003ctd\u003eRound bottom minimizes cell loss during aspiration of supernatant\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003cp\u003e\u003cem\u003eNatural (clear) PS for suspension cell culture and visual agglutination reads. The U-bottom geometry is the defining feature — color choice is secondary to bottom geometry for these applications.\u003c\/em\u003e\u003c\/p\u003e \u003ch2\u003eChemical Resistance — Polystyrene (PS)\u003c\/h2\u003e \u003cp\u003ePS provides excellent resistance to aqueous assay reagents. Not autoclave-compatible. Avoid acetone and chlorinated solvents.\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eReagent \/ Condition\u003c\/th\u003e \u003cth\u003eCompatibility\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eAqueous buffers (PBS, TBS, HEPES)\u003c\/td\u003e \u003ctd\u003e✓ Excellent\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCell culture media (DMEM, RPMI)\u003c\/td\u003e \u003ctd\u003e✓ Excellent\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDMSO (≤10%)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eShort-chain alcohols (brief)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDilute acids \/ alkalies (\u0026lt;10%)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFormalin (4%, fixation step)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAcetone\u003c\/td\u003e \u003ctd\u003e✗ Avoid\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eChlorinated solvents\u003c\/td\u003e \u003ctd\u003e✗ Avoid\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAutoclave (121°C)\u003c\/td\u003e \u003ctd\u003e✗ Not compatible\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eLaboratory Applications\u003c\/h2\u003e \u003cp\u003eThe U-bottom plate serves immunology and hematology labs running HAI hemagglutination inhibition and viral neutralization assays; cell biology programs culturing suspension lymphocytes, PBMCs, and hybridomas; microbiology labs running MIC broth microdilution; cytokine bead array and multiplex bead assay programs; immunoprecipitation workflows; and any application requiring cells or particles to settle uniformly by gravity.\u003c\/p\u003e \u003ch2\u003eU.S. Manufacturing, Quality \u0026amp; Institutional Procurement\u003c\/h2\u003e \u003cp\u003eEvery Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. \u003ca href=\"#request-sample\"\u003eRequest a sample below\u003c\/a\u003e to validate before committing to volume.\u003c\/p\u003e \u003ch2\u003eFrequently Asked Questions — Caplugs® Evergreen \u003c\/h2\u003e \u003ch3\u003eWhy use U-bottom instead of flat-bottom for suspension cells?\u003c\/h3\u003e \u003cp\u003eIn a flat-bottom well, suspension cells distribute across the entire flat floor — making complete aspiration difficult without disturbing the cell layer. The U-bottom geometry allows cells to settle by gravity into a uniform central pellet at the well bottom, enabling clean supernatant aspiration and minimal cell loss during wash steps. This is the defining requirement for HAI assays (where RBC pellet button vs streaming is the read), hybridoma culture, and PBMC-based assays.\u003c\/p\u003e \u003ch3\u003eIs the plate the standard format for HAI assays?\u003c\/h3\u003e \u003cp\u003eYes. Round (U-bottom) 96-well PS plates are the WHO-specified format for hemagglutination inhibition (HAI) and viral neutralization assays. The U-bottom geometry allows red blood cells to settle into a uniform button pattern that can be visually scored (button = inhibition; streaming\/shield = hemagglutination). Flat-bottom plates produce irregular RBC distribution that is difficult to score reliably.\u003c\/p\u003e \u003ch3\u003eIs the compatible with automated plate readers for cell-based assays?\u003c\/h3\u003e \u003cp\u003eYes, with the caveat that U-bottom geometry affects path length for absorbance reads. U-bottom plates are primarily used for visual agglutination scoring, luminescence, and fluorescence intensity detection modes — not for quantitative absorbance ELISA where flat-bottom is required for accurate path length. The SBS\/ANSI footprint is compatible with all standard plate readers for luminescence and fluorescence detection.\u003c\/p\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"96-Well Microplates, Round (U-Bottom) Bottom, 0.32 mL Well Volume, Non-Sterile, Polystyrene (PS), Natural \\u2014 290-8117-01R | 10\/Box (100\/Case) | Caplugs\\u00ae Evergreen\",\n  \"description\": \"Caplugs\\u00ae Evergreen 290-8117-01R. 96-well U-bottom microplate, PS natural, 0.32 mL, 10\/Box (100\/Case). Cell culture, assays. BPA-free. 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