{"product_id":"caplugs-290-8027-w1f-96-well-microplate-flat-bottom-ps-white-370ul","title":"100–200 µL Microplates, Flat Bottom, PS, White — 290-8027-W1F | 9\/Box | Caplugs® Evergreen","description":"\u003ch2\u003e96-Well Flat Bottom Microplates, 0.37 mL Well Volume, PS, White — 290-8027-W1F | 9\/Box (90\/Case)\u003c\/h2\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e\u003cth colspan=\"2\"\u003eQuick Reference — 290-8027-W1F\u003c\/th\u003e\u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eWell Format\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e96-well, SBS\/ANSI standard footprint\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eBottom\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eFlat Bottom\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eWell Volume\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e0.37 mL\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eColor\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eWhite (enhanced luminescence \/ absorbance)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003eSterility\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003eNon-Sterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e9 \/ Box (90 \/ Case)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e\u003cstrong\u003ePrice\u003c\/strong\u003e\u003c\/td\u003e \u003ctd\u003e$127.63 \/ Box ($1.4181 \/ plate)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eTechnical Narrative\u003c\/h2\u003e \u003cp\u003eThe Caplugs® Evergreen is a 96-well flat-bottom white Polystyrene (PS) microplate in the SBS\/ANSI standard footprint — the workhorse format for ELISA, luminescence, chemiluminescence, and absorbance-based quantitative assays in clinical, research, and high-throughput screening environments. The white well color maximizes luminescence and chemiluminescence signal by reflecting photons from well walls back toward the plate reader detector, providing 2–5-fold higher signal versus equivalent clear or black plates for luciferase reporter assays, ATP bioluminescence, and chemiluminescent ELISA formats. The flat bottom ensures uniform path length across the full well volume for absorbance-based colorimetric ELISA and kinetic enzyme assays.\u003c\/p\u003e \u003cp\u003eThe 0.37 mL well volume provides the standard working capacity for most 96-well assay formats — accommodating reagent addition sequences, wash steps, and final detection volumes across ELISA (typically 100–200 µL\/well), luciferase assays (20–100 µL\/well), and cell proliferation assays (100–200 µL\/well) without overflow risk. PS construction is compatible with all standard aqueous assay buffers, culture media, and detection reagents including HRP substrates (TMB, OPD), AP substrates (pNPP), and luciferase substrates.\u003c\/p\u003e \u003cp\u003eAt $127.63 per Box of 9 — $1.4181 per plate — the delivers white PS flat-bottom microplate economics for high-throughput programs running 10–100+ plates per week. Also available: 290-8028-W1F (96-well flat PS White — sterile variant); 290-8029-W1R (96-well round-bottom PS White).\u003c\/p\u003e \u003ch2\u003eTechnical Specifications — Caplugs® Evergreen \u003c\/h2\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eSpecification\u003c\/th\u003e \u003cth\u003eValue\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003ePart Number\u003c\/td\u003e \u003ctd\u003e\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCaplugs Item #\u003c\/td\u003e \u003ctd\u003e2908027W1FE1\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eWell Format\u003c\/td\u003e \u003ctd\u003e96-well, SBS\/ANSI standard footprint\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eBottom Geometry\u003c\/td\u003e \u003ctd\u003eFlat Bottom\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eWell Volume\u003c\/td\u003e \u003ctd\u003e0.37 mL (per well, working volume ~0.1–0.3 mL)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eMaterial\u003c\/td\u003e \u003ctd\u003ePolystyrene (PS)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eWell Color\u003c\/td\u003e \u003ctd\u003eWhite\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eWhite Plate Use\u003c\/td\u003e \u003ctd\u003eEnhanced luminescence, chemiluminescence, absorbance bottom-read\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eSterility\u003c\/td\u003e \u003ctd\u003eNon-Sterile\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003ePack Size\u003c\/td\u003e \u003ctd\u003e9 \/ Box (90 \/ Case)\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eReorder Unit\u003c\/td\u003e \u003ctd\u003eBox of 9 or Case of 90\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eApplication\u003c\/td\u003e \u003ctd\u003eELISA, luminescence, chemiluminescence, absorbance, cell proliferation\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eBPA \/ Phthalate\u003c\/td\u003e \u003ctd\u003eBPA-free; no phthalate plasticizers\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCountry of Origin\u003c\/td\u003e \u003ctd\u003eMade in U.S.A. (California)\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eAssay \u0026amp; Application Compatibility — 96-Well Flat Bottom White PS\u003c\/h2\u003e \u003cp\u003eWhite PS flat-bottom 96-well plates are the standard format for luminescence, chemiluminescence, and absorbance-based assays. Compatible workflows:\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eApplication\u003c\/th\u003e \u003cth\u003eAssay \/ Use\u003c\/th\u003e \u003cth\u003eNotes\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eELISA \/ colorimetric assays\u003c\/td\u003e \u003ctd\u003eOD absorbance at 405, 450, 490, 570 nm\u003c\/td\u003e \u003ctd\u003eWhite PS maximizes bottom read luminescence — ideal for chemiluminescence\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCell proliferation assays\u003c\/td\u003e \u003ctd\u003eMTT, MTS, WST-1, BrdU incorporation\u003c\/td\u003e \u003ctd\u003eFlat bottom essential for absorbance-based cell viability reads\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eLuminescence assays\u003c\/td\u003e \u003ctd\u003eLuciferase reporter, ATP bioluminescence\u003c\/td\u003e \u003ctd\u003eWhite well walls maximize signal reflection for top and bottom read\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eFluorescence intensity (FI)\u003c\/td\u003e \u003ctd\u003eGFP, RFP, fluorescent dye quantification\u003c\/td\u003e \u003ctd\u003eWhite plate for fluorescence intensity — not recommended for FRET\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDNA \/ RNA quantification\u003c\/td\u003e \u003ctd\u003ePicoGreen, RiboGreen, SYBR fluorescent dyes\u003c\/td\u003e \u003ctd\u003eFlat bottom suits spectrophotometric well-plate DNA quantification\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eImmunoassay MSD \/ Meso Scale\u003c\/td\u003e \u003ctd\u003eElectrochemiluminescence plate-based assays\u003c\/td\u003e \u003ctd\u003eFlat bottom required for MSD sector imager electrode contact\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eHigh-content screening\u003c\/td\u003e \u003ctd\u003ePhenotypic cell imaging at plate level\u003c\/td\u003e \u003ctd\u003eValidate flat-bottom optical quality for imaging-based assays\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAbsorbance kinetics\u003c\/td\u003e \u003ctd\u003eEnzyme kinetics, substrate consumption\u003c\/td\u003e \u003ctd\u003eFlat bottom ensures consistent path length across well volume\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003cp\u003e\u003cem\u003eWhite plates enhance luminescence and chemiluminescence signal by reflecting photons from the well walls back toward the detector. For fluorescence assays where well cross-talk is a concern, black plates are preferred. For cell-based imaging, clear-bottom plates are required.\u003c\/em\u003e\u003c\/p\u003e \u003ch2\u003eChemical Resistance — Polystyrene (PS)\u003c\/h2\u003e \u003cp\u003eWhite PS provides maximum luminescence signal enhancement. Not autoclave-compatible. Avoid acetone and chlorinated solvents.\u003c\/p\u003e \u003ctable\u003e \u003cthead\u003e\u003ctr\u003e \u003cth\u003eReagent \/ Condition\u003c\/th\u003e \u003cth\u003eCompatibility\u003c\/th\u003e \u003c\/tr\u003e\u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003eAqueous buffers (PBS, TBS, citrate)\u003c\/td\u003e \u003ctd\u003e✓ Excellent\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eCell culture media\u003c\/td\u003e \u003ctd\u003e✓ Excellent\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDMSO (≤10%)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eShort-chain alcohols (brief)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eDilute acids \/ alkalies (\u0026lt;10%)\u003c\/td\u003e \u003ctd\u003e✓ Good\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAcetone\u003c\/td\u003e \u003ctd\u003e✗ Avoid\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eChlorinated solvents\u003c\/td\u003e \u003ctd\u003e✗ Avoid\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003eAutoclave (121°C)\u003c\/td\u003e \u003ctd\u003e✗ Not compatible — white PS\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e \u003ch2\u003eLaboratory Applications\u003c\/h2\u003e \u003cp\u003eThe serves immunology and infectious disease labs running ELISA-based antibody and antigen quantification; drug discovery programs using luciferase reporter assays and ATP bioluminescence viability; clinical diagnostic labs running chemiluminescent immunoassay (CLIA) formats; biochemistry labs performing enzyme kinetic and substrate consumption assays; cell biology programs measuring proliferation and cytotoxicity with colorimetric dyes (MTT, MTS, WST-1); genomics and proteomics labs quantifying nucleic acids and proteins by fluorescent dye plate assays; and any research or clinical setting requiring a high-performance white PS flat-bottom 96-well plate at bulk economics.\u003c\/p\u003e \u003ch2\u003eU.S. Manufacturing, Quality \u0026amp; Institutional Procurement\u003c\/h2\u003e \u003cp\u003eEvery Caplugs® Evergreen lot is precision-molded at our California manufacturing facility and subject to rigorous dimensional inspection and material purity verification. We supply reference laboratories, hospital systems, biotech firms, and federal procurement offices under institutional pricing programs with blanket-order support and same-day shipping on stocked inventory. \u003ca href=\"#request-sample\"\u003eRequest a sample below\u003c\/a\u003e to validate before committing to volume.\u003c\/p\u003e \u003ch2\u003eFrequently Asked Questions — Caplugs® Evergreen \u003c\/h2\u003e \u003ch3\u003eWhy use a white plate versus a clear or black plate for ELISA and luminescence?\u003c\/h3\u003e \u003cp\u003ePlate color determines how photons are handled in the well. White plates reflect light from well walls back toward the detector — maximizing signal for luminescence (luciferase, ATP bioluminescence) and chemiluminescence (ECL ELISA, CLIA) assays by 2–5x versus clear. Clear plates transmit light — used for absorbance assays requiring transmission-mode reads or cell-based imaging. Black plates absorb stray light — used for fluorescence assays where well cross-talk and background autofluorescence must be minimized. For standard ELISA with HRP\/TMB or luminescence-based assays, white is the correct choice for maximum sensitivity.\u003c\/p\u003e \u003ch3\u003eIs the plate compatible with automated plate readers?\u003c\/h3\u003e \u003cp\u003eYes. The conforms to the SBS\/ANSI 96-well microplate standard footprint (127.76 × 85.47 mm), ensuring compatibility with all standard microplate readers including SpectraMax, PHERAstar, Synergy (BioTek\/Agilent), Infinite (Tecan), and EnVision (PerkinElmer). The flat bottom supports both top-read and bottom-read absorbance, fluorescence intensity, and luminescence detection modes.\u003c\/p\u003e \u003ch3\u003eIs the microplate sterile?\u003c\/h3\u003e \u003cp\u003eThe is Non-Sterile as supplied — the standard specification for ELISA, luciferase, and biochemical assay plates where assay reagents are the primary contamination control, not container sterility. For cell culture applications requiring sterile plates, Caplugs® Evergreen sterile variants (290-8028 family) are available. White PS is not autoclave-compatible — do not attempt to sterilize by autoclaving. Use gamma or UV sterilization if required.\u003c\/p\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"96-Well Microplates, Flat Bottom, 0.37 mL Well Volume, Non-Sterile, Polystyrene (PS), White \\u2014 290-8027-W1F | 9\/Box (90\/Case) | Caplugs\\u00ae Evergreen\",\n  \"description\": \"Caplugs\\u00ae Evergreen 290-8027-W1F. 96-well flat bottom microplate, PS white, 0.37 mL volume, 9\/box (90\/case). ELISA, luminescence. USA.\",\n  \"sku\": \"2908027W1FE1\",\n  \"mpn\": \"290-8027-W1F\",\n  \"material\": \"Polystyrene (PS)\",\n  \"color\": \"White\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"Caplugs\\u00ae Evergreen\"\n  },\n  \"manufacturer\": {\n    \"@type\": \"Organization\",\n    \"name\": \"Caplugs\\u00ae Evergreen\",\n    \"address\": {\n      \"@type\": \"PostalAddress\",\n      \"addressCountry\": \"US\",\n      \"addressRegion\": \"CA\"\n    }\n  },\n  \"offers\": {\n    \"@type\": \"Offer\",\n    \"price\": \"127.63\",\n    \"priceCurrency\": \"USD\",\n    \"availability\": \"https:\/\/schema.org\/InStock\",\n    \"itemCondition\": \"https:\/\/schema.org\/NewCondition\",\n    \"seller\": {\n      \"@type\": \"Organization\",\n      \"name\": \"LabSupplies\"\n    }\n  },\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Pack Size\",\n      \"value\": \"90 \/ Box\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Sterility\",\n      \"value\": \"Non-Sterile\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Country of Origin\",\n      \"value\": \"United States\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"BPA Free\",\n      \"value\": \"Yes\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Well Format\",\n      \"value\": \"96-well SBS\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Bottom Geometry\",\n      \"value\": \"Flat Bottom\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Well Volume\",\n      \"value\": \"0.37 mL\"\n    }\n  ]\n}\n\u003c\/script\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Why use a white plate versus a clear or black plate for ELISA and luminescence?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Plate color determines how photons are handled in the well. White plates reflect light from well walls back toward the detector \\u2014 maximizing signal for luminescence (luciferase, ATP bioluminescence) and chemiluminescence (ECL ELISA, CLIA) assays by 2\\u20135x versus clear. Clear plates transmit light \\u2014 used for absorbance assays requiring transmission-mode reads or cell-based imaging. Black plates absorb stray light \\u2014 used for fluorescence assays where well cross-talk and background autofluorescence must be minimized. For standard ELISA with HRP\/TMB or luminescence-based assays, white is the correct choice for maximum sensitivity.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Is the 290-8027-W1F plate compatible with automated plate readers?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes. The 290-8027-W1F conforms to the SBS\/ANSI 96-well microplate standard footprint (127.76 \\u00d7 85.47 mm), ensuring compatibility with all standard microplate readers including SpectraMax, PHERAstar, Synergy (BioTek\/Agilent), Infinite (Tecan), and EnVision (PerkinElmer). The flat bottom supports both top-read and bottom-read absorbance, fluorescence intensity, and luminescence detection modes.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Is the 290-8027-W1F microplate sterile?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The 290-8027-W1F is Non-Sterile as supplied \\u2014 the standard specification for ELISA, luciferase, and biochemical assay plates where assay reagents are the primary contamination control, not container sterility. For cell culture applications requiring sterile plates, Caplugs\\u00ae Evergreen sterile variants (290-8028 family) are available. 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